Status of giant clam resources around Okinawa‐jima Island,Ryukyu Archipelago,Japan

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Protein expression profiling in haemocytes and plasma of the Manila clam Ruditapes philippinarum in response to infection with Perkinsus olseni

The protein expression profiling in clam haemocytes and plasma in response to Perkinsus olseni was addressed. Adult Manila clams from a P. olseni‐free bed were experimentally challenged with parasite zoospores to analyse immune response. In another experiment, the effects of longer term infection were assessed in adult clams collected from a P. olseni‐affected bed, by comparing moderate to very heavily infected clams with non‐infected ones. Haemocyte and plasma proteins were separated by two‐dimensional electrophoresis; spot patterns were qualitatively compared between treatments within each experiment and the spots indicating differential protein expression associated with P. olseni challenge or with field infection were processed for protein identification. Fifteen clam proteins (four in haemocytes and eleven in plasma) of which expression was markedly affected by P. olseni were identified. Some of the identified proteins have a well‐known role in clam immune response against the parasite, such as lysozyme and lectins. Rho GTPase‐activating protein 6 could be a marker of resistance against P. olseni, which should be further studied.     

Cytotoxic Effect on Human Cancer Cells and Antioxidant Activity of Extracts of <Emphasis Type="Italic">Codonopsis lanceolata</Emphasis> Root using Different Solvent Fractions

This study was conducted to investigate cytotoxic effect, phenolic content, DPPH, ABTS radical scavenging rate and nitrite scavenging rate from different solvent fractions of Codonopsis lanceolate root. At all extracts concentration, the cytotoxic effect on different fractions against human cancer cells was higher in n-hexane and butyl alcohol fractions than in the other fractions. The IC₅₀ value on HeLa cell showed the lowest by 62.70 μg.mL^-1 on n-hexane fraction, and exhibited the values of butyl alcohol fraction 341.36 μg.mL^-1, methylene chloride 598.33 μg.mL^-1, ethyl acetate fraction 860.44 μg.mL^-1, DW fraction 2896.82 μg.mL^-1. Total polyphenol content on different solvent fractions varied from 102.43 to 153.52 mg.g^-1, and that of ethyl acetate fraction showed the highest amount. The DPPH and ABTS radical scavenging activity showed that the increase was proportional to the concentration, and the scavenging activity also showed the highest in ethyl acetate fraction. The nitrite scavenging activity of each fraction at pH 1.2 was in the order of EA > BA > MC > n-H > DW, and the lower the acidity, the higher nitrite scavenging activity, and there was no distinct detection of nitrite scavenging effects of the pH range 6.0. The results of this study suggested that the extract of Codonopsis lanceolate root may assist in the potential biological activities, and it was found that the activity was different depending on the organic solvent fraction and the water fraction.     

茶业电子商务的现状与对策

本文从茶商的角度,分析电子商务时代的到来给茶商带来什么样的机会,介绍当前茶业电子商务的现状,剖析茶业电子商务应用过程中存在的问题,提出茶商应如何开展电子商务,最后展望茶业电子商务的前景。     

Difference in Allelopathic Potential as Influenced by Root Periderm Colour of Sweet Potato (Ipomoea batatas)

Laboratory bioassay and high‐performance liquid chromatography (HPLC) analysis were conducted to determine the allelopathic potentials of aqueous or methanol extracts from three different coloured sweet potato [Ipomoea batatas L. (Lam)] cultivars by plant part. The aqueous extracts applied on filter paper significantly inhibited root growth of alfalfa (Medicago sativa L.). Aqueous leaf leachates at 40 g dry tissue l^?1 (g l^?1) from white sweet potato cultivar ‘Sinyulmi’ showed the highest inhibition against alfalfa, followed by yellow ‘Sinhwangmi’ and purple ‘Jami’. Alfalfa root growth was significantly inhibited by methanol extracts of the same plants as the concentration increased. Aqueous and methanol extracts from leaves showed the most inhibitory effect on alfalfa root growth followed by stems and roots. By means of HPLC analysis, leaf samples of sweet potato had the highest amount of phenolic compounds followed by stems and roots. Total content of these compounds was highest for leaf extracts (37.7 mg 100 g^?1), detected in EtOAc fraction, especially trans‐cinnamic acid (20.9 mg 100 g^?1). These results suggest that sweet potato plants are allelopathic and that their activities differ depending on plant part as well as root periderm colour.     

Cancer-preventive peptide lunasin from Solanum nigrum L. inhibits acetylation of core histones H3 and H4 and phosphorylation of retinoblastoma protein (Rb)

Lunasin, a unique 43 amino acid, 4.8 kDa cancer-chemopreventive peptide initially reported in soybean and now found in barley and wheat, has been shown to be cancer-chemopreventive in mammalian cells and in a skin cancer mouse model against oncogenes and chemical carcinogens. To identify bioactive components in traditional herbal medicines and in search for new sources of lunasin, we report here the properties of lunasin from Solanum nigrum L. (SNL), a plant indigenous to northeast Asia. Lunasin was screened in the crude extracts of five varieties of the medicinal plants of Solanaceae origin and seven other major herbal plants. An in vitro digestion stability assay for measuring bioavailability was carried out on SNL crude protein and autoclaved SNL using pepsin and pancreatin. A nonradioactive histone acetyltransferase (HAT) assay and HAT activity colorimetric assay were used to measure the inhibition of core histone acetylation. The inhibitory effect of lunasin on the phosphorylation of retinoblastoma protein (Rb) was determined by immunoblotting against phospho-Rb. Lunasin isolated from autoclaved SNL inhibited core histone H3 and H4 acetylation, the activities of the HATs, and the phosphorylation of the Rb protein. Lunasin in the crude protein and in the autoclaved crude protein was very stable to pepsin and pancreatin in vitro digestion, while the synthetic pure lunasin was digested at 2 min after the reaction. We conclude that lunasin is a bioactive and bioavailable component in SNL and that consumption of SNL may play an important role in cancer prevention.     

"Concepts in animal welfare": a syllabus in animal welfare science and ethics for veterinary schools

Public attitudes toward animal welfare have improved with growing social affluence, and veterinarians are increasingly expected to be informed about animal welfare in a broader sense than health alone. However, animal welfare has not been a traditional component of the veterinary curriculum. To help address this lack, the World Society for the Protection of Animals(WSPA) and the University of Bristol School of Veterinary Science launched the 'Concepts in Animal Welfare' syllabus in 2003. This comprehensive syllabus comprises seven core and 23 elective modules and covers a range of animal welfare issues, including farm and companion animal welfare, wildlife, and the use of animals in experiments. There are also modules on ethics and animal legislation. The syllabus is interactive, promotes critical analysis of issues from different angles, and may be adapted for use in any veterinary curriculum. WSPA provides training and workshops in developing countries and assists with the implementation of the syllabus.     

Protein Profiling from Hormone-Induced Tetraploid Roots in Platycodon grandiflorum

Journal of Crop Science and Biotechnology - The root of Platycodon grandiflorum is used as traditional oriental medicine in Asian countries since ancient times to treat bronchitis, tonsillitis,...     

Effects of Nitrogen,Phosphorus and Potassium Fertilizers on Growth Characteristics of Two Species of Bellflower (Platycodon grandiflorum)

Journal of Crop Science and Biotechnology - Fertilization plays a vital role to enhance productivity in several medicinal plants, including Bellflower. A pot experiment was conducted in 2018 to...     

Protein-binding affinity of leucaena condensed tannins of differing molecular weights

Depending on their source, concentration, chemical structure, and molecular weight, condensed tannins (CTs) form insoluble complexes with protein, which could lead to ruminal bypass protein, benefiting animal production. In this study, CTs from Leuceana leucocephala hybrid were fractionated into five fractions by a size exclusion chromatography procedure. The molecular weights of the CT fractions were determined using Q-TOF LC-MS, and the protein-binding affinities of the respective CT fractions were determined using a protein precipitation assay with bovine serum albumin (BSA) as the standard protein. The calculated number-average molecular weights (M(n)) were 1348.6, 857.1, 730.1, 726.0, and 497.1, and b values (the b value represents the CT quantity that is needed to bind half of the maximum precipitable BSA) of the different molecular weight fractions were 0.381, 0.510, 0.580, 0.636, and 0.780 for fractions 1, 2, 3, 4, and 5, respectively. The results indicated that, in general, CTs of higher molecular weight fractions have stronger protein-binding affinity than those of lower molecular weights. However, the number of hydroxyl units within the structure of CT polymers also affects the protein-binding affinity.