全文获取类型
收费全文 | 17768篇 |
免费 | 70篇 |
国内免费 | 85篇 |
专业分类
林业 | 3786篇 |
农学 | 1450篇 |
基础科学 | 229篇 |
2875篇 | |
综合类 | 1183篇 |
农作物 | 2173篇 |
水产渔业 | 1833篇 |
畜牧兽医 | 1253篇 |
园艺 | 1220篇 |
植物保护 | 1921篇 |
出版年
2024年 | 1篇 |
2023年 | 6篇 |
2022年 | 59篇 |
2021年 | 63篇 |
2020年 | 74篇 |
2019年 | 65篇 |
2018年 | 2787篇 |
2017年 | 2768篇 |
2016年 | 1235篇 |
2015年 | 141篇 |
2014年 | 84篇 |
2013年 | 102篇 |
2012年 | 926篇 |
2011年 | 2234篇 |
2010年 | 2197篇 |
2009年 | 1337篇 |
2008年 | 1374篇 |
2007年 | 1624篇 |
2006年 | 83篇 |
2005年 | 134篇 |
2004年 | 123篇 |
2003年 | 169篇 |
2002年 | 73篇 |
2001年 | 22篇 |
2000年 | 57篇 |
1999年 | 17篇 |
1998年 | 22篇 |
1997年 | 16篇 |
1996年 | 17篇 |
1995年 | 10篇 |
1994年 | 7篇 |
1993年 | 26篇 |
1992年 | 10篇 |
1991年 | 2篇 |
1990年 | 5篇 |
1989年 | 11篇 |
1988年 | 13篇 |
1987年 | 5篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1977年 | 4篇 |
1972年 | 1篇 |
1969年 | 1篇 |
1968年 | 5篇 |
1967年 | 3篇 |
1966年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
1.
2.
3.
牦牛病毒性腹泻/粘膜病的防制研究 总被引:10,自引:0,他引:10
20世纪80年代以来,我国牦牛群中陆续发现牛病毒性腹泻/粘膜病(BVD/MD),血清阳性率在30%~42.4%之间,病死率在30%左右,本研究先后从四川、西藏等地牦牛中分离出病毒,并对其进行各种生物学特征鉴定后,表明该病毒与标准毒属同一种,所不同的是四川牦牛病毒株属非致细胞病变型,即属NCP型。但回归本动物能复制出典型病例。目前尚无国产牛粘膜病疫苗用于生产。本研究依据猪瘟病毒与牛病毒性腹泻/粘膜病病毒具有交叉免疫性的原理,用猪瘟弱毒苗对牦牛病毒性腹泻/粘膜病进行预防,试验证明用猪瘟弱毒苗可以预防牛病毒性腹泻/粘膜病,且安全可靠,具有实用价值。 相似文献
4.
5.
廖莎 《湖南农业大学学报(自然科学版)》1996,23(6)
通过2榀预应力混凝土叠合框架1:3比例的模型试验,了解了叠合梁框架在竖向荷载作用下的受力性能。讨论了叠合梁截面应变分布特点,并分析了梁的配筋率这一主要参数对框架极限承载力和破坏形态的影响。 相似文献
6.
7.
鸭病毒性肝炎鸭胚灭活疫苗的研究 总被引:6,自引:0,他引:6
以鸭病毒性肝炎病毒Ⅰ型ATCC毒株接种健康鸭胚,收集致死的全胚组织作制苗材料,用福尔马林灭活,以麸氨酸钠终止其作用。先后试制疫苗11批,在试验室免疫雏鸭71只,经强毒攻击后,总的保护率为90.4%。本疫苗免疫雏鸭3天后可产生较坚强的免疫力,在室温至少可保存11天,在4℃可保存254天。经在江苏、安徽、广东等省推广应用200000头剂,均安全有效,颇受用户欢迎,对控制鸭病毒性肝炎的流行起了重要作用。 相似文献
8.
A. Schots J. De Boer A. Schouten J. Roosien J. F. Zil Verentant H. Pomp L. Bouwman-Smits H. Overmars F. J. Gommers B. Visser W. J. Stiekema J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191
Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (VL) and heavy chain (VH) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location. 相似文献
9.
Shohei MATSUURA Shigeru HOSHINO Hideaki HAYASHI Tetsuyuki KOHGUCHI Kyoji HAGIWARA Toshihiro OMURA 《Journal of General Plant Pathology》2002,68(1):99-102
DAS-ELISA proved to be reliable enough to detect a latent infection by Tomato spotted wilt virus (TSWV) in asymptomatic stock plants of chrysanthemum. A high density of Frankliniella occidentalis, the predominant vector, in the presence of latently infected stock plants resulted in a high incidence of disease in the chrysanthemum
production field. The incidence of disease was low when the vector thrips were not abundant in spite of the presence of latently
infected stock plants. These results suggest that an infestation of the vector thrips causes severe secondary spread of TSWV
originating from latently infected stock plants in chrysanthemum production fields.
Received 27 July 2001/ Accepted in revised form 27 November 2001 相似文献
10.