全文获取类型
收费全文 | 441篇 |
免费 | 26篇 |
专业分类
林业 | 43篇 |
农学 | 22篇 |
72篇 | |
综合类 | 8篇 |
农作物 | 31篇 |
水产渔业 | 78篇 |
畜牧兽医 | 178篇 |
园艺 | 7篇 |
植物保护 | 28篇 |
出版年
2023年 | 7篇 |
2022年 | 24篇 |
2021年 | 32篇 |
2020年 | 30篇 |
2019年 | 26篇 |
2018年 | 35篇 |
2017年 | 33篇 |
2016年 | 30篇 |
2015年 | 13篇 |
2014年 | 32篇 |
2013年 | 28篇 |
2012年 | 29篇 |
2011年 | 38篇 |
2010年 | 19篇 |
2009年 | 12篇 |
2008年 | 15篇 |
2007年 | 14篇 |
2006年 | 12篇 |
2005年 | 6篇 |
2004年 | 11篇 |
2003年 | 6篇 |
2002年 | 6篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1996年 | 1篇 |
1993年 | 1篇 |
排序方式: 共有467条查询结果,搜索用时 640 毫秒
1.
de Carvalho Menezes de Almeida Sheylla Foligno Souza-Fabjan Joanna Maria Gonçalves Balaro Mario Felipe Alvarez Bragança Gláucia Mota Pinto Pedro Henrique Nicolau de Almeida José Gabriel Moura Ana Beatriz Bossois da Fonseca Jeferson Ferreira Brandão Felipe Zandonadi 《Tropical animal health and production》2018,50(2):427-432
Tropical Animal Health and Production - This study evaluated the effect of two doses of prostaglandin at different intervals on reproductive parameters of crossbred ewes. In Experiment 1, 30 ewes... 相似文献
2.
Paula de Aguiar Silva Gleidson Giordano Pinto de Carvalho Aureliano José Vieira Pires Stefanie Alvarenga Santos Douglas dos Santos Pina Robério Rodrigues Silva Carlindo Santos Rodrigues Luis Henrique Almeida de Matos Carlos Emanuel Eiras Daiane Novais-Eiras Wilson Souza Nunes 《Tropical animal health and production》2018,50(4):721-726
In this study, we evaluated the effects of including cassava wastewater in the diet on the feeding behavior of feedlot lambs in 35 male uncastrated Santa Inês × Dorper crossbred lambs at an approximate age of 3 months, with an average live weight of 20.0?±?3.4 kg. Diets were formulated with hay of cassava shoots (roughage) and a concentrate based on corn and soybean, with a roughage:concentrate ratio of 50:50, plus inclusion of cassava wastewater at the levels of 0, 12, 24, 36, or 48 g/kg of the total diet. Feeding behavior was evaluated between the 46th and 52nd days of the experiment. Increasing cassava wastewater levels in the diet reduced (P?<?0.05) the intakes (kg/day) of dry matter and neutral detergent fiber as well as the efficiency of rumination (g/cud and g/h) of dry matter and neutral detergent fiber. The other behavioral parameters were not affected by wastewater inclusion in the diet. Therefore, the inclusion of up to 48 g/kg of cassava wastewater on fresh matter of diets is not recommended for feedlot lambs. 相似文献
3.
Julia Belotto Guaraná Alois Foltran Müller Ricardo de Francisco Strefezzi Fabrício Singaretti Oliveira Luciana Cristina Machado Carlos Eduardo Ambrósio Renata Gebara Sampaio Dória Silvio Henrique de Freitas 《Anatomia, histologia, embryologia》2021,50(6):996-1006
The use of live animals for educational purposes is an old practice that is still employed in teaching and research institutions. However, there are several objections to this practice, whether for ethical or humanitarian reasons. Surgical techniques teaching using anatomical pieces and/or preserved cadavers promotes greater learning efficiency, provides exercise repetition and increases the confidence and satisfaction of the students when compared to the use of live animals. The current work aimed to analyse the feasibility of using fresh swine urinary bladder and small intestines (jejunum), obtained from slaughterhouses, fixed in 99.8% ethyl alcohol (EA) and preserved in sodium chloride hypersaturated solution (SCHS) at 30%, for 7, 14 and 21 days, as an alternative method for surgical skills training (SST). Swine viscera, fixed in EA and preserved in SCHS, presented a realistic appearance, absence of odour and maintained the viable morphological characteristics during the performance of the operative techniques. Preservation solutions had low cost, were easy to acquire and did not offers risks to human health. Therefore, urinary bladders and small intestines fixed in 99.8% EA for 30 days and maintained in 30% SCHS at different periods were demonstrated as a good viable option as a preservation method for surgical skills training. 相似文献
4.
5.
Aguiar PH Barrouin-Melo SM Franke CR dos Santos RR Silva TM Mengel JO dos-Santos WL Pontes-de-Carvalho L 《Veterinary journal (London, England : 1997)》2007,173(1):158-166
This report describes the characterisation of a monoclonal antibody (mAb), AB6, which recognises specifically a cluster of canine leukocyte surface molecules. The immunogen used for obtaining the AB6 mAb was a lysate of canine peripheral blood mononuclear cells (PBMC). This novel mAb belongs to the IgG2a isotype, and reacted in Western blot with four different canine leukocyte glycoproteins with apparent molecular weights of 180, 190, 205 and 220 kDa. The AB6 mAb recognised the majority of canine peripheral blood leukocytes as determined by flow cytometry (97%). It also exhibited a broad reactivity pattern against lymphoid and myeloid cells, inhibited the proliferation of mitogen-stimulated canine PBMC and did not recognise human PBMC and murine splenocytes. The biochemical properties, cell and tissue specificity, and in vitro biological activity of the AB6 mAb indicate that it recognises a canine CD45 homologue. The mAb could become a valuable diagnostic and research tool for the evaluation of immune functions in dogs. 相似文献
6.
Henrique de Souza Luche José Antonio Gonzalez da Silva Rafael Nornberg Emilio Ghisleni Arenhardt Vanderlei da Rosa Caetano Luciano Carlos da Maia Antonio Costa de Oliveira 《Journal of Crop Science and Biotechnology》2017,20(2):73-80
In wheat, the increase of yield and stability associated traits can be achieved by combining parents containing the stay-green trait and favorable alleles for grain yield. The aim of this work was to analyze the genetic dissimilarity between wheat lines from stay-green and synchronized maturation groups and elite cultivars. Moreover, to propose promising combinations seeking the selection of high-grain yield and high bread-making quality genotypes containing stay-green trait. The experiment was conducted in a randomized block design with three replications in 2003, 2004, and 2005, using sister-lines with the presence and absence of stay-green trait and elite cultivars. Genetic variability exists among wheat strains from the synchronized stay-green maturation group and elite cultivars. Genotypes of maturation group stay-green obtained an average performance superior to the synchronized group. Crosses between stay-green lines and the CEP 29 and BRS 177 cultivars are promising in the selection of genotypes carrying the stay-green trait with high yield and bread-making quality. 相似文献
7.
8.
Vinicius T. Franceschi Kaique S. Alves Sergio M. Mazaro Cláudia V. Godoy Henrique S. S. Duarte Emerson M. Del Ponte 《Plant pathology》2020,69(3):495-505
Soybean rust (SBR), caused by Phakopsora pachyrhizi, is the most important yield-damaging fungal disease of soybean due to severe reduction in healthy leaf area and acceleration of leaf fall. In experimental research, SBR severity is estimated visually aided/trained by a standard area diagram (SAD) developed and validated during the mid-2000s (Old SAD). In this study, we propose a new SAD set for SBR with six true-colour diagrams following linear increments (c. 15% increments) amended with four additional diagrams at low (<10%) severities, totalling 10 diagrams (0.2%, 1%, 3%, 5%, 10%, 25%, 40%, 55%, 70%, and 84%). For evaluation, 37 raters were split into two groups. Each assessed severity in a 50-image sample (0.25%–84%), first unaided and then using either the Old SAD or the New SAD. Accuracy, precision, and reliability of estimates improved significantly relative to unaided estimates only when aided by the New SAD (accuracy >0.95). Low precision (<0.78) and a trend of underestimation with an increase in severity were the main issues with the Old SAD, which did not differ from unaided estimates. Simulation to evaluate the impact of the errors by different methods on hypothesis tests, showed that the new SAD was more powerful for detecting the smallest difference in mean control (e.g., 70% vs. 65% disease reduction) than the Old SAD; the latter required a 2-fold increase in sample size to achieve the same power. There is a need to improve some SADs, taking advantage of new knowledge and technology to increase accuracy of the estimates, and to optimize both resource use efficiency and management decisions. 相似文献
9.
Frederic Vandeperre Ruth M. Higgins Julio Sánchez‐Meca Francesc Maynou Raquel Goñi Pablo Martín‐Sosa Angel Pérez‐Ruzafa Pedro Afonso Iacopo Bertocci Romain Crec’hriou Giovanni D’Anna Mark Dimech Carmelo Dorta Oscar Esparza Jesús M. Falcón Aitor Forcada Ivan Guala Laurence Le Direach Concepción Marcos Celia Ojeda‐Martínez Carlo Pipitone Patrick J. Schembri Vanessa Stelzenmüller Ben Stobart Ricardo S. Santos 《Fish and Fisheries》2011,12(4):412-426
Marine protected areas (MPAs) are often promoted as tools for biodiversity conservation as well as for fisheries management. Despite increasing evidence of their usefulness, questions remain regarding the optimal design of MPAs, in particular concerning their function as fisheries management tools, for which empirical studies are still lacking. Using 28 data sets from seven MPAs in Southern Europe, we developed a meta‐analytical approach to investigate the effects of protection on adjacent fisheries and asking how these effects are influenced by MPA size and age. Southern European MPAs showed clear effects on the surrounding fisheries, on the ‘catch per unit effort’ (CPUE) of target species, but especially on the CPUE of the marketable catch. These effects depended on the time of protection and on the size of the no‐take area. CPUE of both target species and the marketable catch increased gradually by 2–4% per year over a long time period (at least 30 years). The influence of the size of the no‐take area appeared to be more complex. The catch rates of the entire fishery in and around the MPA were higher when the no‐take areas were smaller. Conversely, catch rates of selected fisheries that were expected to benefit most from protection increased when the no‐take area was larger. Our results emphasize the importance of MPA size on its export functions and suggest that an adequate, often extended, time frame be used for the management and the evaluation of effectiveness of MPAs. 相似文献
10.
Izquierdo MS Robaina L Juárez-Carrillo E Oliva V Hernández-Cruz CM Afonso JM 《Fish physiology and biochemistry》2008,34(2):117-127
The Δ6 and Δ5 desaturases and elongases show only very limited activity in marine fish, and little is known of the possibility
of enhancing Δ6 desaturase gene expression in these fish. The use of plant oils in marine fish diets is limited by their lack
of n−3 highly unsaturated fatty acids (HUFA) despite an abundant content of the 18C fatty acid precursor linoleic and α-linolenic
acids. The objective of the present study was to determine the ability of larval gilthead seabream to utilize vegetable oils
and assess the nutritional regulation of Δ6 desaturase gene expression. Seventeen-day-old gilthead seabream larvae were fed
during a 17-day period with one of four different microdiets formulated with either sardine fish oil (FO), soybean, rapeseed
or linseed oils, respectively, or a fifth diet containing defatted squid meal and linseed oil. Good larval survival and growth,
both in terms of total length and body weight, were obtained by feeding the larvae either rapeseed, soybean or linseed oils.
The presence of vegetable oils in the diet increased the levels of 20:2n−9 and 20:2n−6, 18:2n−9, 18:3n−6, 20:3n−6 and 20:4n−6, in larvae fed rapeseed and soybean oils in comparison to those fed FO. In addition, a sixfold increase in the relative
expression of Δ6 desaturase-like gene was found in larvae fed rapeseed and soybean oils, denoting the nutritional regulation
of desaturase activity through its gene expression in this fish species. However, feeding linseed oil did not increase the
expression of the Δ6 desaturase gene to such a high extent. 相似文献