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排序方式: 共有169条查询结果,搜索用时 15 毫秒
1.
Hiroto Miura Takuya Hashimoto Yukiko Kawanishi Hiroki Kawauchi Ryo Inoue Noriaki Shoji Kunihiko Saito Mario Sekiya Yosuke Saito Jumpei Yasuda Chiemi Yonezawa Tetsushiro Endo Hirotaka Kasuya Yutaka Suzuki Yasuo Kobayashi Satoshi Koike 《Animal Science Journal》2021,92(1):e13601
The rumen microbiota comprises a vast range of bacterial taxa, which may affect the production of high-quality meat in Japanese Black cattle. The aim of this study was to identify core rumen microbiota in rumen fluid samples collected from 74 Japanese Black cattle raised under different dietary conditions using 16S rRNA gene amplicon sequencing. In the rumen of fattening Japanese Black cattle, 10 bacterial taxa, showing >1% average relative abundance and >95% prevalence, irrespective of the dietary conditions and the fattening periods, were identified as the core rumen bacterial taxa, which accounted for approximately 80% of the rumen microbiota in Japanese Black cattle. Additionally, population dynamics of the core rumen bacterial taxa revealed two distinct patterns: Prevotella spp. and unclassified Bacteroidales decreased in the mid-fattening period, whereas unclassified Clostridiales, unclassified Ruminococcaceae, Ruminococcus spp., and unclassified Christensenellaceae increased during the same period. Therefore, the present study reports the wide distribution of the core rumen bacterial taxa in Japanese Black cattle, and the complementary nature of the population dynamics of these core taxa, which may ensure stable rumen fermentation during the fattening period. 相似文献
2.
Takao Tsukiboshi Yoshiaki Chikuo Yoko Ito Yosuke Matsushita Koji Kageyama 《Journal of General Plant Pathology》2007,73(4):293-296
Root and stem rot with wilt of above ground parts of cultivated chrysanthemums was first found in Ibaraki, Toyama and Kagawa
prefectures, Japan in 2002 and 2003. Pythium species were isolated from the diseased tissues and identified as P. dissotocum, P. oedochilum, P. sylvaticum, P. ultimum var. ultimum and asexual strains of P. helicoides based on their morphologies and sequences of rDNA-ITS region. All the Pythium species were strongly pathogenic to chrysanthemums in pot conditions and were reisolated from the inoculated plants. Because
Pythium root and stem rot of chrysanthemum has never been reported in Japan, we propose that this is a new disease that can
be caused by the five Pythium species. 相似文献
3.
Tadahisa MASHITA Hiroaki KAMISHINA Yuya NAKAMOTO Yosuke AKAGI Ataru NAKANISHI Yusuke HARASAKI Tsuyoshi OZAWA Takashi UEMURA Yui KOBATAKE Shunsuke SHIMAMURA Naoki KITAMURA Sadatoshi MAEDA Yuji UZUKA Gerry SHAW Jun YASUDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):433-438
The aim of this study was to evaluate the prognostic value of concurrent measurement of
serum phosphorylated neurofilament heavy subunit (pNF-H) concentration and intramedullary
T2W hyperintensity in paraplegic to paraplegic dogs. Our hypothesis was that concurrent
measurement of these would provide a more accurate prediction of functional outcome in
dogs with thoracolumbar intervertebral disc herniation (IVDH). A prospective case-control
clinical study was designed using 94 dogs with acute onset of thoracolumbar IVDH. The
association of serum pNF-H concentration, T2W hyperintensity on sagittal MRI (T2H/L2),
deep pain perception and surgical outcome were evaluated with logistic regression analysis
after three months for all 94 surgically treated dogs. Sensitivity to predict
non-ambulatory outcome was compared among pNF-H and T2H/L2 and combination of both.
Logistic regression analysis indicated that serum pNF-H concentration and T2H/L2 were
significantly correlated with surgical outcome (P<0.05); however, deep
pain perception was not (P=0.41). The results of logistic regression
analysis indicated that the odds ratios of unsuccessful long-term outcome were 2.6 for
serum pNF-H concentration, 1.9 for T2H/L2 and 2.3 for deep pain sensation. The sensitivity
and specificity to predict non-ambulatory outcome for using serum parameter pNF-H>2.6
ng/ml, using T2H/L2 value of>0.84 and using both
serum pNF-H and T2H/L2, were 95% and 75.7%, 65% and 86.5%, and 90.0% and 97.5%,
respectively. Therefore, combined measurements of serum pNF-H and T2H/L2 might be useful
for predicting long-term outcome in dogs with thoracolumbar IVDH. 相似文献
4.
Yosuke Sasaki Tadaaki Tokunaga Ryoko Uemura Masuo Sueyoshi 《Animal Science Journal》2014,85(3):213-218
We investigated the reproductive and lifetime performances of Kagoshima Berkshire gilts and sows. We examined 20 605 parity records of 4419 pigs for 2008 to 2012 on a farrow‐to‐finish commercial farm. The mean parity (± SD) of all animals was 3.0 ± 2.1. For farrowing performance, the highest numbers of total pigs born and pigs born alive were found in sows with parities 5 and 6 and with parity 3–6, respectively (P < 0.05). Regarding weaning and mating performance, sows with parity 2 had the lowest preweaning mortality (P < 0.05). The longest weaning‐to‐first‐mating interval was found in parity 1 pigs, and the interval decreased as parity increased (P < 0.05). Parities 0 and 1 pigs had the lowest farrowing rate and those with parity 4 had the highest farrowing rate (P < 0.05). The mean parity at culling, total number of pigs born alive in a lifetime, and nonproductive days in a lifetime were 5.5 ± 2.93, 49.2 ± 24.72 pigs, and 132.1 ± 83.34 days, respectively. These animals had a lower litter size and fertility that the F1 crossbred sows mainly used in Japan, but a similar tendency for performance by parity. 相似文献
5.
RFLP analysis of a PCR amplified region of chloroplast DNA in eggplant and related Solanum species 总被引:1,自引:0,他引:1
RFLP analysis of a PCR amplified 3.2-kbp region of cpDNA bounded by the conserved sequences in rbc L and ORF 106 was performed
in eggplant (Solanum melongena), its related Solanum species, S. incanum, S. virginianum (= S. surattense), S. torvum, S.
aethiopicum (= S. gilo), S. aethiopicum (= S. integrifolium), S. violaceum (= S. indicum), S. violaceum (= S. sanitwongsei)
and S. mammosum and the reciprocal hybrids between S. aethiopicum (= S. integrifolium) and S. melongena 'Uttara'. The target
region of cpDNA was amplified correctly by PCR. The amplified products were digested with each of 10 restriction enzymes (Alu
I, Ase I, BamH I, Hinf I, Msp I, Rsa I, ScrF I, Sty I, Taq I and Xba I). Variations of restriction patterns among the species
were recognized after digesting the amplified products with each of the seven restriction enzymes, Taq I, Alu I, Rsa I, Sty
I, Ase I, Hinf I and Xba I. The restriction patterns divided the examined nine species into the following five clusters, 1)
S. melongena and S. incanum, 2) S. virginianum (= S. surattense), 3) S. torvum, 4) S. aethiopicum (= S. gilo), S. aethiopicum
(= S. integrifolium), S. violaceum (= S. indicum) and S. violaceum (= S. sanitwongsei) and 5) S. mammosum. The restriction
pattern with Alu I in each of the reciprocal hybrids between S. melongena 'Uttara' and S. aethiopicum (= S. integrifolium)
was identical with that of seed parent. The present study demonstrated the availability of the PCR-RFLP analysis of cpDNA
for assessing taxonomic relationships and identifying cytoplasmic parentage of interspecific hybrids in eggplant and related
Solanum species.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
6.
7.
8.
In a previous study, we developed male sterile lines of Japanese bunching onion (Allium fistulosum L.) possessing the cytoplasm of a wild species, A. galanthum Kar. et Kir., by backcrossing. To evaluate seed productivity of the male sterile lines in practise, they were crossed with the male fertile line, cultivar 'Kujyo', using honeybees as pollinators under field conditions. The number of florets and seeds per inflorescence, seed set and seed germination of the material were investigated. Although variation was observed among the male sterile lines, there were several lines having seed productivity equal to cultivar 'Kujyo'. Our data demonstrate that the male sterile lines of A. fistulosum possessing the cytoplasm of A. galanthum are useful as seed parents for the commercial F seed production of A. fistulosum. 相似文献
9.
10.
The effect of soil freeze–thaw cycles on the denitrification potential was examined based on the C2H2 inhibition method. The gross N2O production curve of the soil sample (incubation with C2H2) showed minor changes between the freeze–thaw treatment and the unfrozen control. However, kinetics analysis revealed that
the initial production rate, an indicator of the population density of denitrifying communities, decreased (P = 0.043) and the specific growth rate constant, an indicator of the activity of denitrifying communities, increased (P = 0.039) as a result of the freeze–thaw cycles in five of six soil samples examined. The increase in the specific growth
rate constant suggested the stimulation of the activity of denitrifying communities that survived after the freeze–thaw cycles
and may explain the minor suppression on the gross N2O production in spite of decreasing the population density of denitrifying communities that was suggested by the initial production
rate. The net N2O production curve of the soil sample (incubation without C2H2) showed a remarkable change in one out of six soil samples, and in that one soil sample, N2O release to the atmosphere was largely stimulated (7.6 times) by the freeze–thaw cycles. However, the stimulation of the
N2O release by the freeze–thaw cycles was even observed in two other selected soil samples (4.6 and 1.8 times), suggesting that
an imbalance in the N2O-producing and N2O-reducing activities of denitrifying communities might complementally explain the N2O release stimulated by the freeze–thaw cycles. 相似文献