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排序方式: 共有196条查询结果,搜索用时 46 毫秒
1.
T Hashizume T Sasaki S Nonaka T Hayashi M Takisawa M Horiuchi T Hirata E Kasuya 《Reproduction in domestic animals》2005,40(2):184-189
It has been reported that the posterior pituitary (PP) gland contains a potent, unknown prolactin (PRL)-releasing factor (PRF) in rats. PRFs are assumed to be produced in neurones located within the hypothalamus, and to be peptidergic in nature. However, little is known about PRFs in domestic animals. To characterize the PRF in the PP of domestic animals, the present study examined the PRL-releasing activity of an acidic extract from bovine PP (bPP) in vitro and in vivo in cattle. First, the PRL-releasing effect of bPP extract was compared with that of PRL-releasing peptide (PrRP), and thyrotropin-releasing hormone (TRH) from cultured bovine anterior pituitary cells. The extract significantly increased PRL concentrations in the culture medium, at doses of 0.002 and 0.02 eq./ml (one eq. is the PP extract from one animal), compared with the control (p < 0.05). PrRP failed to stimulate the release of PRL. TRH significantly increased PRL concentrations in the culture medium, at doses from 10(-9) to 10(-7) M, compared with the control (p < 0.05). The rate of increase in the PRL concentration, by 0.02 eq./ml bPP extract, was significantly greater than that in TRH (p < 0.05). Secondly, plasma PRL responses to the intravenous (i.v.) injection of bPP extract (0.5 eq./head), PrRP [3.59 mug/kg body weight (BW)], TRH (1 mug/kg BW), and a dopamine receptor antagonist (sulpiride, 0.1 mg/kg BW), were examined in calves. PrRP failed to stimulate PRL release; however, plasma PRL increased immediately following the injection of bPP extract, TRH and sulpiride. The PRL-releasing effect of i.v. injections of TRH and sulpiride was more potent than that of bPP extract. Finally, plasma PRL responses to the intra-hypothalamic injection of bPP extract were examined in calves. The intra-hypothalamic infusion (arcuate nucleus) of 0.0625 eq./head of bPP extract strongly stimulated PRL release in calves (p < 0.05). The present results show that PP contains a physiologically potent PRF in cattle. 相似文献
2.
Eiji Tanaka Chihiro Tanaka Atsushi Ishihara Yasumasa Kuwahara Mitsuya Tsuda 《Journal of General Plant Pathology》2003,69(1):1-6
Aciculosporium take (Ascomycota; Clavicipitaceae) is a causal agent of witches' broom of
bamboo plants. The symptoms of this disease are believed to be induced by plant
hormones, particularly auxins. Indole-3-acetic acid (IAA) was identified in
cultures of this fungus in an l-tryptophan-supplemented liquid medium.
IAA production was confirmed on 30 isolates of A. take from various
hosts and locations at levels up to 1 mg/l. The biosynthetic pathway of
IAA in A. take culture was examined by analyzing intermediate products
and by feeding experiments. The results showed that the indole-3-pyruvic acid
pathway (l-tryptophan → indole-3-pyruvic acid → indole
acetaldehyde → IAA) was the dominant pathway in A. take.
Received: June 3, 2002 / Accepted: July 25, 2002 相似文献
3.
Sward characteristics,nutritive value and choice by cattle of conterminous monocultures of centipedegrass (Eremochloa ophiuroides) and bahiagrass (Paspalum notatum)
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Masahiko Hirata Toshiro Okuma Yuichiro Tanaka Manabu Tobisa 《Animal Science Journal》2016,87(5):674-680
In south‐western Japan, centipedegrass (Eremochloa ophiuroides; CG) offers a novel option for a warm‐season perennial for grazing use in areas where bahiagrass (Paspalum notatum; BG) can be grown. However, the potential of CG as a forage has not been fully explored because of the short history as a forage crop. We conducted four experiments to evaluate CG (cv. TifBlair) in comparison with BG (cv. Pensacola) in terms of sward characteristics, nutritive value and choice by animals. In each experiment, four Japanese Black cows (Bos taurus) were individually allowed to graze conterminous monocultures of CG and BG (5 × 10 m each) for 30 min. Irrespective of regrowth durations and fertilizer rates, CG was consistently shorter, leafier and denser, contained lower acid detergent fiber and cellulose, and was preferred or equally selected by cows, as compared with BG. Furthermore, CG maintained sufficient levels of crude protein (80–89 g/kg DM) to ensure voluntary intake of ruminant animals under extended regrowth? and without fertilizer, whereas BG failed to do so (65 g/kg DM). CG provided higher digestible dry matter than BG when crude protein concentration exceeded 86 g/kg DM. The results indicate advantages of CG as a forage. 相似文献
4.
Masaharu Komatsu Naoki Ichiyama Takashi Kurimoto Shota Takumi Kazuhiro Shiozaki Yasumasa Sugiyama Tatsuhiko Furukawa Seiichi Ando Saki Itonori Hiroaki Saito 《Fisheries Science》2013,79(2):313-320
We have previously established the method for isolation of ceramide aminoethylphosphonate (CAEP) from jumbo flying squid Dosidicus gigas. In this study, we performed a MTT assay to evaluate the safety of CAEP to the cell lines for the application to health food and supplements. The CAEP did not show any cytotoxicity to various HEK293-transfectant cells. Next, we elucidated the positive function of CAEP to the somatic cells. Recently, we have reported that hepatotoxin microcystin-LR was taken up into the hepatocytes mediated by hepatocellular uptake transporters OATP1B1 and OATP1B3, and the cells were induced cytotoxicity subsequently. Cytotoxicity of microcystin-LR to permanently OATP1B3-expressing HEK293-OATP1B3 cells rather than to HEK293-OATP1B1 cells was preferentially attenuated by CAEP in a concentration-dependent manner. In addition, the enzyme activity of serine/threonine phosphatase, which was inhibited by microcystin-LR, was recuperated by co-exposure to CAEP. Furthermore, microcystin-LR-induced cellular protein phosphorylation were disrupted by CAEP exposure. These results suggested that CAEP is a promising remedy and/or preventive medicine for liver damage with microcystin-LR. 相似文献
5.
Glomus-wetland rice mycorrhizas influenced by nursery inoculation techniques under high fertility soil conditions 总被引:2,自引:0,他引:2
We investigated the effect of nursery inoculation techniques on mycorrhizal colonization and sporulation, growth responses,
and nutrient (N and P) uptake to determine the suitable nursey inoculation method of wetland rice (Oryza sativa L.) under high-fertility soil conditions. Seedlings were produced in dry-nursery (DN, watered to 60% of –0.03 MPa) and wet-nursery
(WN, 3–5 cm water from the soil surface) conditions with or without arbuscular mycorrhizal fungal (Glomus spp.) inoculation. Soil was γ-ray sterilized before use in this experiment. Mycorrhizal fungal colonization was 56% in DN
and 23% in WN plants at 6 weeks of growth. The arbuscular mycorrhizal fungal colonization was significantly higher in plants
of DN origin than in WN plants after transplantation to the pots, irrespective of growing stages. Mycorrhizal colonization
was significantly decreased to 28% in DN plants and to 25% in WN plants at harvest. The grain yield was significantly influenced
by nursery conditions. N and P acquisition of wetland rice plants inoculated with Glomus spp. was significantly greater than that of non-inoculated plants at maturity, especially in those originating from DN conditions.
P translocation from shoots to grain was accelerated by mycorrhizas.
Received: 6 April 1997 相似文献
6.
Suppressive effect of saturated acyl L-ascorbate on the oxidation of linoleic acid encapsulated with maltodextrin or gum arabic by spray-drying 总被引:2,自引:0,他引:2
Watanabe Y Fang X Minemoto Y Adachi S Matsuno R 《Journal of agricultural and food chemistry》2002,50(14):3984-3987
6-O-Palmitoyl L-ascorbate was added to linoleic acid at various molar ratios of the ascorbate to the acid, the mixtures were emulsified with a maltodextrin or gum arabic solution, and the emulsions were spray-dried to produce microcapsules. At higher molar ratios, the oil droplets in the emulsions were smaller, and the oxidative stabilities of the encapsulated linoleic acid were higher for both the maltodextrin- and gum arabic-based microcapsules. 6-O-Capryloyl, caproyl, and lauroyl L-ascorbates, which were synthesized through lipase-catalyzed condensation in acetone, were also used for the microencapsulation of linoleic acid. Except for capryloyl L-ascorbate, the addition of a saturated acyl ascorbate, especially caproyl ascorbate, to linoleic acid was effective for preparing oil droplets of small particle diameter and for suppressing the oxidation of the encapsulated linoleic acid. 相似文献
7.
日本冲绳岛琉球松林台风干扰后的群落特点 总被引:3,自引:0,他引:3
为阐述亚热带地区琉球松林受到干扰后的演替规律,就日本冲绳地区琉球松人工林在强台风干扰后,林分结构和树种组成特点进行了调查分析。结果表明,琉球松林经台风干扰后演替形成两种群落类型,即琉球松-峨嵋木荷群落和琉球松-峨嵋木荷-蕨类群落;两者在林分结构和树种组成特点方面差异明显。琉球松-峨嵋木荷群落中,出现乔灌树种26种(DBH≥3.0cm),树种多样性指数为2.55,林下植被特别是草本层盖度低,没有蕨类植物分布。而琉球松-峨嵋木荷-蕨类群落中,出现乔灌树种33种,树种多样性指数为3.51,林下植被特别是草本层发育较好,并有大量蕨类植物分布,其盖度达55%。此外,经受干扰的琉球松林出现了大量的较耐荫的常绿阔叶树种,尤其是山茶科和樟科的树种,而没有出现本地天然常绿阔叶林的优势树种壳斗科的栲类(Castanopsis spp.)。充分说明强台风干扰促进了琉球松林向常绿阔叶林演替。 相似文献
8.
Hashizume K Takahashi T Shimizu M Todoroki J Shimada A Hirata M Sato T Ito A 《The Journal of reproduction and development》2003,49(1):45-53
In vitro cell culture is a convenient tool for studying cellular mechanisms. In the present study, production of matrix-metalloproteinases (MMPs) in bovine endometrial (containing both epithelial and stromal cells) monolayer cells was examined. Blastocysts attached to the endometrial cells in a monolayer culture were examined for their effects on MMP-2 production. Initial attachment of blastocysts to the monolayer inhibited MMP-2 production by endometrial cells. But once trophoblast cells began to migrate into the endometrial cell layer, MMP-2 production increased, and at the same time MMP-9 production also became evident in the medium. In order to understand how blastocysts affected MMP-2 production, we examined the effect of progesterone, estradiol, insulin-like growth factors (IGFs), tumor necrosis factors (TNFs), and interferon-tau (IFN-tau) supplementation. It was IFN-tau that inhibited the production of MMP-2. In addition, progesterone at a lower dose appeared to inhibit MMP-2 production. Both TNF-alpha and TNF-beta strongly stimulated the production of MMP-2 and MMP-9, whereas IGFs had no effect. Based on these findings, it appears that conceptus has the capacity to inhibit MMP activity. 相似文献
9.
For better understanding wood hardness, we developed a new hand-operated hardness tester that works with Brinell's method. With this tester we investigated the hardness distribution of wood minutely using a 2mm diameter ball tip. The following results were obtained: (1) On preliminary examination with medium-density fiberboard, we found that the value of Brinell's hardness decreased with the increase in the tip ball's diameter but that it was almost constant with the indenting velocity. (2) By using a small diameter tip, the difference of the hardness became clearer between earlywood and latewood. (3) With radial and tangential sections, we obtained hardness distribution patterns similar to those of wood grain. It seems that the hardness distribution reflects the distribution of density on wood surfaces. 相似文献
10.
Tetsuji Ota Tsuyoshi Kajisa Nobuya Mizoue Shigejiro Yoshida Gen Takao Yasumasa Hirata Naoyuki Furuya Takio Sano Raul Ponce-Hernandez Oumer S. Ahmed Heng Sokh Vuthy Ma Eriko Ito Jumpei Toriyama Yukako Monda Hideki Saito Yoshiyuki Kiyono Sophal Chann Nang Ket 《Journal of Forest Research》2015,20(6):484-492