Regulatory Effect of let-7f Transfection in Non-Small Cell Lung Cancer on its Candidate Target Genes

Background:Let-7f has essential impacts on biological processes; however, its biological and molecular functions in lung cancer pathogenesis have yet been remained unclear. We aimed to investigate the expression level of let-7f and its candidate target genes both in lung cancer tissues and A549 cell line. Methods:Bioinformatics databases were first used to select candidate target genes of let-7f. Then the relative gene and protein expressions of let-7f and its target genes, including HMGA2, ARID3B, SMARCAD1, and FZD3, were measured in lung tissues of Non-Small Cell Lung Cancer (NSCLC) patients and A549 cell line using quantitative real-time PCR and Western blotting. The electroporation method was used to transfect A549 cells with let-7f mimic and microRNA inhibitor. The impact of let-7f transfection on the viability of A549 cells was assessed using MTT assay. The expression data of studied genes were analyzed statistically Results:Results indicated significant downregulated expression level of let-7f-5p (p = 0.0013) and upregulated level of the HMGA2 and FZD3 in NSCLC cases (p < 0.05). In A549 cells, after transfection with let-7f mimic, the expression of both mRNA and protein levels of HMGA2, ARID3B, SMARCAD1, and FZD3 decreased. Also, the overexpression of let-7f significantly inhibited the A549 cell proliferation and viability (p = 0.017). Conclusion:Our findings exhibited the high value of let-7f and HMGA2 as biomarkers for NSCLC. The let-7f, as a major tumor suppressor regulatory factor via direct targeting genes (e.g. HMGA2), inhibits lung cancer cell viability and proliferation and could serve as a marker for the early diagnostic of NSCLC.Key Words: MicroRNAs, Biomarkers, Carcinoma, Non-Small-Cell Lung     

Both soybean and kudzu phytoestrogens modify favorably the blood lipoprotein profile in ovariectomized and castrated hamsters

The present study compared the hypolipidemic activity of kudzu phytoestrogens with that of soybean phytoestrogen in estrogen- and androgen-deficient hamsters. In the first experiment, ovariectomized hamsters (n = 37) were randomly divided into four groups (n = 9-10 each group). The first group was the control group, whereas the second group had the time-releasing estradiol-17beta subcutaneous (pellet) implants as a positive control. The third and fourth groups were orally administered soybean or kudzu phytoestrogen extracts (30 mg/kg of body weight) per day. In the second experiments, the first group of male hamsters (n = 9) received a sham operation, whereas the other three groups of male hamsters (n = 9 each) were castrated. The castrated control group received orally distilled water, whereas the second and third castrated groups were orally given 30 mg/kg soybean or kudzu phytoestrogen extracts. The results for the first experiment showed that the ovariectomized hamsters orally given soybean and kudzu phytoestrogen extracts had significantly decreased serum total cholesterol (TC) and non-high-density lipoprotein cholesterol (non-HDL-C) with HDL cholesterol (HDL-C) being unaffected. The data from the second experiment demonstrated that administration of soybean but not kudzu phytoestrogen extracts decreased significantly serum TC. However, administration of kudzu phytoestrogens caused redistribution of cholesterol among lipoproteins, leading to a significant decrease in the ratio of non-HDL-C to HDL-C. It was concluded that both soybean and kudzu phytoestrogens could modify favorably lipoprotein profiles in ovariectomized and castrated hamsters.     

The effect of pest control on the incidence of trichinosis in Virovitica-Podravina County, Croatia

The aim of the study was to assess the role of pest control as a preventive measure in the control of trichinosis. The investigation was designed as a retrospective cohort study in the Virovitica-Podravina County. The incidence of trichinosis was determined according to the presence or absence of exposure to protective pest control measures. Differences in the rate of trichinosis infection were determined by calculating relative risk (RR), i.e., the ratio of absolute risk in the two groups. In this epidemiological study, data collected in the County area regularly covered by pest control (exposure area) were compared with other areas not covered by regular pest control measures (non-exposure area). The study included 132 subjects living in the County and locally infected with trichinosis. Patient data were obtained from their medical history, epidemiological survey and epidemiological questionnaire on the source (autochthony) of the disease. The study also included 133,028 domestic pigs, 875 wild animals (mostly wild boar) and 111 trapped rats. All samples were examined by the method of trichinoscopy at authorized veterinary institutions of the County. Trichinosis infection was recorded in 1012 of 79,534 domestic pigs in the area exposed to pest control measures and in 51 of 53,494 domestic pigs from the rest of the County. RR for the exposure area vs. non-exposure area was 13.35 (95% CI 10.08-17.68; p<0.05); according to particular areas, RR was 3.66 (95% CI 2.62-5.13) for Pitomaca, 22.47 (95% CI 13.27-38.08) for Slatina and 118.89 (95% CI 29.7-476.01) for Orahovica (p<0.05 all). Domestic pigs from the County areas exposed to regular pest control measures had a 13-fold likelihood of infection recorded in pigs from County areas where pest control measures were not or were only occasionally performed. Quite unexpectedly, these results revealed a failure of pest control to reduce the incidence of trichinosis. The spread of trichinosis obviously occurs by some routes obviating the impact of pest control.     

Operational Effectiveness of Centralized Nursery for Small-Scale Forestry in San Carlos City, Negros Occidental, the Philippines: Lessons Learned in Seedling Production for Commercial Energy Crop Plantations

One way to maximize potential income of smallholder tree farmers is to ensure high quality of planting material. In San Carlos City, Negros Occidental, the Philippines, great importance is placed on seedling quality because Energy Crop Plantations (ECPs) managed under a Short-Rotation Coppice (SRC) system will involve a single planting for several three-year coppices. High seedling quality requires superior seed provenances and seedling propagation methods. Throughout the establishment of the nursery facility, and the propagation of planting stock, data collection, reporting and database systems have been designed to capture relevant costs and technical information. Training has been provided to nursery personnel and labourers to enable them to operate the nursery and produce high quality seedlings. This paper reports a practical operational evaluation of all materials and procedures followed in nursery establishment and seedling production. Economies of scale in nursery operation are achieved at a production level of at least 90,000 seedlings per planting season of three months in the year. The production cost is estimated at PhP 3.57/seedling, excluding the cost of the nursery infrastructure and overheads. Cost comparison with decentralized or contracted nurseries shows little variation at a total cost of PhP 4.12/seedling. However, with these alternative nursery arrangements there is high risk of producing low quality seedlings by possible reduction of inputs and skipping quality enhancement procedures to reduce production cost.