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1.
Chie SUZUKI Yosuke SAKAGUCHI Hiroyoshi HOSHI Koji YOSHIOKA 《The Journal of reproduction and development》2016,62(1):79-86
The effects of lipid-rich bovine serum albumin (LR-BSA) on the development of porcine blastocysts produced
in vitro were examined. Addition of 0.5 to 5 mg/ml LR-BSA to porcine blastocyst medium
(PBM) from Day 5 (Day 0 = in vitro fertilization) significantly increased the hatching rates
of blastocysts on Day 7 and the total cell numbers in Day-7 blastocysts. When Day-5 blastocysts were cultured
with PBM alone, PBM containing LR-BSA, recombinant human serum albumin or fatty acid-free BSA, addition of
LR-BSA significantly enhanced hatching rates and the cell number in blastocysts that survived compared with
other treatments. The diameter, ATP content and numbers of both inner cell mass and total cells in Day-6 and
Day-7 blastocysts cultured with PBM containing LR-BSA were significantly higher than in blastocysts cultured
with PBM alone, whereas LR-BSA had no effect on mitochondrial membrane potential. The mRNA levels of enzymes
involved in fatty acid metabolism and β-oxidation (ACSL1, ACSL3,
CPT1, CPT2 and KAT) in Day-7 blastocysts were
significantly upregulated by the addition of LR-BSA. The results indicated that LR-BSA enhanced hatching
ability and quality of porcine blastocysts produced in vitro, as determined by ATP content,
blastocyst diameter and expression levels of the specific genes, suggesting that the stimulatory effects of
LR-BSA arise from lipids bound to albumin. 相似文献
2.
Takuyuki Yoshioka Kazuhiro Aruga Toshio Nitami Hiroshi Kobayashi Hideo Sakai 《Journal of Forest Research》2005,10(2):125-134
Using the method of a life cycle inventory (LCI) analysis, the energy balance and the carbon dioxide (CO2) emission of logging residues from Japanese conventional forestry as alternative energy resources were analyzed over the entire life cycle of the residues. The fuel consumption for forestry machines was measured in field experiments for harvesting and transporting logging residues at forestry operating sites in Japan. In addition, a total audit of energy consumption was undertaken. It involved an assessment of materials, construction, and the repair and maintenance of forestry machines as well as the costs associated with an energy-conversion plant. As a result, the ratio of energy output to input was calculated to be 5.69, indicating that the system examined in this study could be feasible as an energy production system. The CO2 emission per MWhe (e: electricity) of the biomass-fired power generation plant was calculated to be 61.8kgCO2/MWhe, while that of coal-fired power generation plants in Japan is 960kgCO2/MWhe. Therefore, the reduction in the amount of CO2 emission that would result from replacing coal with biomass for power generation by as much as 3.0 million dry-t/year of logging residues in Japan was estimated to be 1.66 million tCO2/year, corresponding to 0.142% of the national CO2 emission. This study provides evidence that Japan could reduce its domestic CO2 emission by using logging residues as alternative energy resources. 相似文献
3.
TAKEYA YOSHIOKA YASUNORI KINOSHITA HIROYUKI YOSHINO SHINHO PARK KUNIHIKO KONNO NOBUO SEKI 《Fisheries Science》2003,69(2):401-407
ABSTRACT: Change in the translucency of squid mantle muscle during itsstorage at 0°C was studied by monitoring L* valueand turbidity. Two indicators showed that squid mantle muscle lostits translucency and reached maximal turbidity within 24 hof storage. Thickness of mantle muscle also increased by 15% in12 h, earlier than the loss of translucency. ATP contentdecreased with storage time and was completely lost in almost 24 h,a similar period to translucency loss, but later than rigor contraction.Development of black color on skin surface by chromatophores wasfully achieved in 24 h. It was thus concluded that ATPcontent was well correlated with a loss of translucency or increasein the turbidity of mantle muscle and development of dark coloron the surface skin of mantle. 相似文献
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Naoto WATANABE Takahisa YAMADA Sachiyo YOSHIOKA Masayuki ITOH Youichi SATOH Masako FURUTA Shigeki KOMATSU Yoshihiko SUMIO Tatsuo FUJITA Yoshiyuki SASAKI 《Animal Science Journal》2010,81(1):142-144
Our previous study detected a single nucleotide polymorphism (SNP), g.1471620G > T , in the 5' flanking region of the endothelial differentiation sphingolipid G-protein-coupled receptor 1 ( EDG1 ) gene, which has been considered as a positional functional candidate for the gene responsible for marbling, and showed association of the g.1471620G > T SNP with marbling in Japanese Black beef cattle. In the present study, we investigated the allele frequency distribution of the g.1471620G > T SNP among the 5 cattle breeds, Japanese Black, Japanese Brown, Japanese Short Horn, Holstein, and Brown Swiss breeds. The T allele at the g.1471620G > T SNP associated with high marbling was found at high frequency in Japanese Black breed that has been subjected to a strong selection for high marbling, while the allele was absent or at very low frequencies in the other breeds that have not been strongly selected for high marbling. Based on this finding, we hypothesized that the pressure of the strong selection for high marbling in Japanese Black breed has increased the frequency of the T allele at the g.1471620G > T SNP in the EDG1 . 相似文献
6.
Shinjiro OJIMA Masashi OKAMURA Nana OSAWA Akiko TAMURA Kazuki YOSHIOKA Takashige KASHIMOTO Takeshi HANEDA Hisaya K. ONO Dong-Liang HU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1147
Salmonella enterica serovar Gallinarum biovar Gallinarum (S. Gallinarum) is a host-specific pathogen causing systemic infection in poultry, which leads to significant economic losses due to high mortality. However, little is known about the dynamic process of systemic infection and pathogenic characteristics of S. Gallinarum in chickens. In the present study, we developed an oral infection model that reproduces the pathology of S. Gallinarum and clarified the host immune response of the infected chickens. Chickens at 20 days of age orally inoculated at a dose of 108 colony forming unit (CFU) showed typical clinical signs of fowl typhoid and died between 6 and 10 days post infection. The inoculated S. Gallinarum rapidly disseminated to multple organs and the bacterial counts increased in the liver and spleen at 3 days post infection. Pathological changes associated wirh inflammation in the liver and spleen became apparent at 4 days post infection, and increased expression of interferon (IFN)-γ and interleuikin (IL)-12 in the liver and spleen did not observed until 3 days post infection. These results indicate that S. Gallinarum rapidly spread to entire body through intestine, and the low-level of inflammatory responses in the liver during the early stage of infection may contribute to rapid, systemic dissemination of the bacteria. Our infection model and findings will contribute to the better understanding of the pathogenic mechanism of S. Gallinarum, and provide new insights into the prevention and control of fowl typhoid. 相似文献
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Shogo HIGAKI Hongyu DARHAN Chie SUZUKI Tomoko SUDA Reina SAKURAI Koji YOSHIOKA 《The Journal of reproduction and development》2021,67(1):67
We aimed to determine the effectiveness of estrus detection based on continuous measurements of the ventral tail base surface temperature (ST) with supervised machine learning in cattle. ST data were obtained through 51 estrus cycles on 11 female cattle (six Holsteins and five Japanese Blacks) using the tail-attached sensor. Three estrus detection models were constructed with the training data (n = 17) using machine learning techniques (random forest, artificial neural network, and support vector machine) based on 13 features extracted from sensing data (indicative of estrus-associated ST changes). Estrus detection abilities of the three models on test data (n = 34) were not statistically different among models in terms of sensitivity and precision (range 50.0% to 58.8% and 60.6% to 73.1%, respectively). The relatively poor performance of the models might indicate the difficulty of separating estrus-associated ST changes from estrus-independent fluctuations in ST. 相似文献
9.
Wataru FUJII Asuka ONUMA Shin YOSHIOKA Keisuke NAGASHIMA Koji SUGIURA Kunihiko NAITO 《The Journal of reproduction and development》2015,61(6):589-593
The generation efficiencies of mutation-induced mice when using engineered zinc-finger nucleases (ZFNs) have
been generally 10 to 20% of obtained pups in previous studies. The discovery of high-affinity DNA-binding
modules can contribute to the generation of various kinds of novel artificial chromatin-targeting tools, such
as zinc-finger acetyltransferases, zinc-finger histone kinases and so on, as well as improvement of reported
zinc-finger recombinases and zinc-finger methyltransferases. Here, we report a novel ZFN pair that has a
highly efficient mutation-induction ability in murine zygotes. The ZFN pair induced mutations in all obtained
mice in the target locus, exon 17 of aminopeptidase Q gene, and almost all of the pups had biallelic
mutations. This high efficiency was also shown in the plasmid DNA transfected in a cultured human cell line.
The induced mutations were inherited normally in the next generation. The zinc-finger modules of this ZFN pair
are expected to contribute to the development of novel ZF-attached chromatin-targeting tools. 相似文献
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