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1.
Nakayama T Yonekura-Sakakibara K Sato T Kikuchi S Fukui Y Fukuchi-Mizutani M Ueda T Nakao M Tanaka Y Kusumi T Nishino T 《Science (New York, N.Y.)》2000,290(5494):1163-1166
Aurones are plant flavonoids that provide yellow color to the flowers of some popular ornamental plants, such as snapdragon and cosmos. In this study, we have identified an enzyme responsible for the synthesis of aurone from chalcones in the yellow snapdragon flower. The enzyme (aureusidin synthase) is a 39-kilodalton, copper-containing glycoprotein catalyzing the hydroxylation and/or oxidative cyclization of the precursor chalcones, 2',4',6',4-tetrahydroxychalcone and 2',4',6',3,4-pentahydroxychalcone. The complementary DNA encoding aureusidin synthase is expressed in the petals of aurone-containing varieties. DNA sequence analysis revealed that aureusidin synthase belongs to the plant polyphenol oxidase family, providing an unequivocal example of the function of the polyphenol oxidase homolog in plants, i.e., flower coloration. 相似文献
2.
Matsubayashi M Carreno RA Tani H Yoshiuchi R Kanai T Kimata I Uni S Furuya M Sasai K 《Veterinary parasitology》2011,176(2-3):270-274
Cystoisospora spp. from feces in dogs, cats, and raccoon dogs were isolated, sequenced at the small subunit ribosomal RNA gene locus and compared to other Cystoisospora spp. Cystoisospora oocysts from dogs and raccoon dogs were morphologically similar with those of C. ohioensis, and cat isolates were similar with those of C. felis. The sequences from dogs and raccoon dogs, and cats have a homology with C. ohioensis and C. felis, respectively. Phylogenetic analysis of the DNA sequences showed that the dog and raccoon dog isolates were nested in a clade with other Cystoisospora spp. including C. ohioensis, C. belli, and C. orlovi. The cat isolate formed a sister group with C. felis that was a separate clade from the dog and raccoon dog group. We report sequence variation in these Cystoisospora sequences and have identified raccoon dogs as another carnivore host for Cystoisospora spp. infecting dogs. 相似文献
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4.
Kanami Mori Takashi Ooi Masanori Hiraoka Naohiro Oka Hideyuki Hamada Mitsumasa Tamura Takenori Kusumi 《Marine drugs》2004,2(2):63-72
Three metabolites of fucoxanthin were isolated from a brown alga, Scytosiphon lomentaria, and the structure of a new compound was determined by NMR. The content of fucoxanthin, a biologically active carotenoid, in four edible brown algae, cultivated in deep seawater, was studied. 相似文献
5.
The abandonment of sites after clearcutting plantations is increasing rapidly in Japan. Although a few stand-level studies
have been conducted, a broad-scale study is needed to understand the general tendency of vegetation recovery and to obtain
information for the management of such extensively distributed abandoned clearcut sites. This study is a province-level study
that aims to interpret the patterns and factors in early-stage vegetation recovery at abandoned plantation clearcut sites.
We also discuss the potential indicators that may determine whether a certain site recovers towards evergreen broad-leaved
forest consisting of Castanopsis cuspidata and Quercus glauca or towards deciduous broad-leaved forest comprising Q. serrata and Q. acutissima. A cluster analysis revealed that five types of vegetation (evergreen trees, evergreen trees–deciduous pioneer shrubs, pioneer
species, deciduous trees, and deciduous shrubs) were observed from an early stage of succession. The first two vegetation
types were considered to recover successfully to the target vegetation of evergreen broad-leaved forest, but the remaining
three vegetation types did not show any tendency to recover to the target vegetations. According to the results of the detrended
correspondence analysis, a smaller size of the abandoned site with adjacent evergreen broad-leaved forest and a longer time
after clearcutting were identified as factors that enhance the invasion of evergreen tree species; these factors are also
useful to predict the future successional tendency. In conjunction with these factors, the existence of regeneration sources
(i.e., stumps) that allow sprouting appeared to be an important indicator for predicting the early-stage vegetation recovery. 相似文献
6.
Keiji OKADA Kazue YAJIMA Takamichi OHBA Tamako MIYAZAKI Takenori ORIHASHI Shigeru SATO Jun YASUDA 《Animal Science Journal》2010,81(1):89-93
Eight Holstein bull calves were divided into two groups; a non-treated control group and a famotidine treated group. Fresh milk was fed twice a day. The experiment was conducted between 7 and 14 days of age. During the experimental period the control group was injected with physiological saline, and the famotidine group was injected with famotidine, a histamine-H2-receptor blocker, into the jugular vein 30 minutes prior to each feeding. The control group showed maximum curd formation 2 h after feeding at both 7 and 14 days of age. Curd scores of 7-day-old and 14-day-old calves were significantly lower in the famotidine than in the control group at 2 and 4 h after feeding. Most fecal samples from the famotidine group exhibited an acidic smell. The famotidine group showed significantly lower values for both average weight gain and the rate of weight gain from 7 to 14 days of age. The inhibition of gastric acid secretion decreased curd formation in the abomasum as well as daily weight gain compared to non-treated control calves. This suggested that curd formation in the abomasum is important for the weight gain of newborn calves. 相似文献
7.
Arisa MUNETOMO Hirotaka ISHII Takenori MIYAMOTO Yasuo SAKUMA Yasuhiko KONDO 《The Journal of reproduction and development》2016,62(1):17-27
In the rat, induction of maternal behavior depends on the parity of the female. For example, nulliparous
(NP) females need longer exposure to pups than multiparous (MP) or lactating (L) females to exhibit similar
maternal behavior. In this study, we investigated the role of brain oxytocin in the approaching behavior of
these female rats. Olfactory preferences for pup odors were examined for 8 consecutive days. Each preference
test was followed by direct overnight exposure to pups. On the 8th day, MP and L, but not NP females showed
robust pup-odor preferences. After the behavioral test, half of the females were exposed to pups for 2 h,
whereas the other half were not. The females were then sacrificed to analyze brain oxytocin (OXT) and
vasopressin (AVP) activities by cFos immunohistochemistry and to quantify their receptor mRNA expression using
real-time PCR. In the paraventricular nucleus (PVN), the percentage of cFos-positive OXT neurons was
significantly larger in MP and L females than in NP females after pup exposure. No significant differences
were found in cFos expression in OXT neurons of the supraoptic nucleus (SON) or in AVP neurons of either the
PVN or SON. Expression of OXT receptor mRNA in the medial preoptic area and amygdala of the control groups was
also higher in MP females than in NP females. Finally, we demonstrated that infusion of OXT into the lateral
ventricle of NP females promoted preferences for pup odors. These results indicate that puerperal and parental
experiences enhance the responsiveness of OXT neurons in the PVN to pup stimuli and establish olfactory
preferences for these odors in a parity-dependent manner. 相似文献
8.
Takenori Kawabata Dhugal J. Lindsay Minoru Kitamura Satoshi Konishi Jun Nishikawa Shuhei Nishida Michiya Kamio Hiroshi Nagai 《Fisheries Science》2013,79(3):487-494
Many polypeptides isolated from shallow water cnidarian species have been utilized as valuable biochemical tools in both basic and applied biological sciences. Deepwater cnidarian species might be another potential resource for novel biochemical tools. However, because of limited access to cnidarian samples from deep-sea environments, bioactive polypeptides have never before been reported from this group. In this study, we collected twelve deep-sea jellyfish species (nine hydrozoans and three scyphozoans) using a plankton net that was specially designed for collecting deep-sea organisms, and prepared water-soluble extracts, presumably containing polypeptides, of these jellyfishes. The extracts were subjected to cytotoxicity, hemolytic activity, and crustacean lethal toxicity tests. In the cytotoxicity test, six out of the nine tested hydrozoan species showed activity. In the hemolytic activity test, only three hydrozoans showed activity and none of the scyphozoan jellyfishes showed activity. In the crustacean lethality test, two hydrozoan jellyfishes and all three of the tested scyphozoan jellyfishes showed lethal activity. These results revealed a high incidence of water-soluble bioactive substances occurring in these deep-sea jellyfishes. Furthermore, all the heat-treated and the methanol-treated crude jellyfish extracts lost their bioactivities. Thus, it is likely that the bioactive compounds in the water-soluble extracts were unstable polypeptides (proteins). This is the first published report on bioactivities in extracts from deep-sea jellyfishes. 相似文献
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Nobuyasu Hanari Hiroshi Yamamoto Takashi Ooi Takenori Kusumi Ken -ichi Kuroda 《Journal of Wood Science》2001,47(1):36-40
This paper describes characterization of diterpenes of the bark of Japanese cypress,Chamaecyparis obtusa (S. and Z.) Endl, without the resinous stem canker to learn the difference between the cypress bark affected with the canker. A diterpene dimer and two diterpenes, 6,12-dihydroxyabieta-8,11,13-trien-7-one and 6,12-dihydroxyabieta-5,8,11, 13-tetraen-7-one, were firstly isolated from Japanese cypress. The dimer, 6-(abieta-6,8,11, 13-tetraenyl-12-oxy)-7-methoxyabieta-8,11,13-trien-12-ol, was a new compound. It is a terpene indicative ofC. obtusa not infected with the resinous stem canker. Five known diterpenes were also isolated. 相似文献