全文获取类型
收费全文 | 1616篇 |
免费 | 179篇 |
专业分类
林业 | 113篇 |
农学 | 70篇 |
基础科学 | 13篇 |
256篇 | |
综合类 | 50篇 |
农作物 | 107篇 |
水产渔业 | 196篇 |
畜牧兽医 | 826篇 |
园艺 | 36篇 |
植物保护 | 128篇 |
出版年
2024年 | 3篇 |
2023年 | 25篇 |
2022年 | 42篇 |
2021年 | 75篇 |
2020年 | 99篇 |
2019年 | 116篇 |
2018年 | 122篇 |
2017年 | 98篇 |
2016年 | 128篇 |
2015年 | 56篇 |
2014年 | 87篇 |
2013年 | 97篇 |
2012年 | 113篇 |
2011年 | 99篇 |
2010年 | 64篇 |
2009年 | 61篇 |
2008年 | 68篇 |
2007年 | 58篇 |
2006年 | 60篇 |
2005年 | 28篇 |
2004年 | 40篇 |
2003年 | 29篇 |
2002年 | 24篇 |
2001年 | 22篇 |
2000年 | 22篇 |
1999年 | 23篇 |
1998年 | 22篇 |
1997年 | 19篇 |
1996年 | 18篇 |
1995年 | 7篇 |
1994年 | 4篇 |
1993年 | 8篇 |
1992年 | 3篇 |
1991年 | 6篇 |
1990年 | 4篇 |
1989年 | 9篇 |
1988年 | 11篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1976年 | 3篇 |
1974年 | 3篇 |
1973年 | 3篇 |
1972年 | 2篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1967年 | 2篇 |
排序方式: 共有1795条查询结果,搜索用时 15 毫秒
1.
Marcilene Fernandes Almeida dos Santos Cleber Furlanetto Maria Rita Alves Almeida Marina Dechechi Gomes Carneiro Fabiane Castro Mota Ana Cristina Meneses Mendes Gomes Natália Orrú Reis Silveira Joelma Gardênia Pereira Silva Philippe Castagnone-Sereno Myrian Silvana Tigano Regina Maria Dechechi Gomes Carneiro 《European journal of plant pathology / European Foundation for Plant Pathology》2012,134(4):671-684
Meloidogyne incognita is one of the most polyphagous species of root-knot nematodes occurring in Brazil and worldwide. Eight M. incognita isolates were studied, representing two enzymatic phenotypes (esterase and malate desydrogenase: I1/N1, I2/N1) and four cryptic Meloidogyne sp.1 (S2/N1) isolates, representing one cytological type (3n?=?40–46). Three M. hispanica isolates (Hi3/N1, 2n?=?32–36) and two of an atypical Meloidogyne sp.2 (S2a/N3, 3n?=?40–44) were included in this study for comparison. All isolates were tested with three M. incognita-specific molecular markers. The primer pairs B06F/R, miF/R and incK14F/R amplified three species-specific fragments of 1,200?bp, 955?bp and 399?bp, respectively for M. incognita and Meloidogyne sp.1 isolates. No amplification occurred in the M. hispanica and Meloidogyne sp.2 isolates, except with primers miF/R (1,650?bp). The genetic variability of the Meloidogyne spp. isolates was evaluated, using RAPD and ISSR markers. The phylogenetic analyses revealed two strongly supported monophyletic clades: clade I, consisting of M. hispanica and the atypical Meloidogyne sp.2 isolates, and clade II, clustering together all M. incognita and the Meloidogyne sp.1 isolates. Considering the biometrical, cytological and molecular approaches, it was possible to conclude that the isolates with three enzymatic phenotypes (I1/N1, I2/N1 and S2/N1) presented the characteristics described for M. incognita. Some correlations were detected between the isozymatic phenotypes and the tree topology (S2a/N3, Hi3/N1, I1/N1, S2/N1), but no strict correlation could be observed for the phenotype I2/N1 and one isolate of S2/N1. Morphologically, the Msp.2 isolates differ from M. incognita and M. hispanica by the female stylet features presenting straight cone tip and round pear shaped knobs, posteriorly sloping. The results of this study suggested that the Msp.2 isolates with phenotypes S2aN3 belong to a new or an unidentified species closely related to M. hispanica. 相似文献
2.
3.
4.
5.
Rui A. Gonçalves Tu Do Cam Nguyen Nhu Tri Gonçalo A. Santos Pedro Encarnação Le Thanh Hung 《Aquaculture International》2018,26(3):921-936
Triplicate groups of one hundred Tra catfish (8 g?±?0.2) were fed seven test diets containing increasing levels of AFB1 (0, 50, 100, 250, 500, and 1000 μg AFB1 kg?1). Additionally Mycofix® Secure was added at 1.5% to one diet containing 500 μg AFB1 kg?1. Results showed that Tra catfish are sensitive to AFB1. Reduction in weight gain (P?<?0.05) was observed for fish fed 50 μg AFB1 kg?1 and declined further with increasing levels of AFB1 in the diets. Fish fed diets contaminated with 500 and 1000 μg AFB1 kg?1 showed increased (P?>?0.05) hepatosomatic index (HIS), while an increase in adipose somatic index (ASI) was observed in fish fed 50 μg AFB1 kg?1 and above when compared to the control and Mycofix® diets. After 12 weeks, blood serum analysis revealed higher alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in fish fed the 50, 100, and 250 μg AFB1 kg?1 suggesting occurrence of liver damage. Disease resistance of fish exposed to Edwardsiella ictaluri was also compromised by the presence of AFB1 in the feed and was directly related to the contamination level. Seven days after Edwardsiella ictaluri exposure, survival rates were 50, 41.7, 31.7, and 8.3% for fish fed control, 50, 100, and 250 μg AFB1 kg?1, respectively. This trial shows that AFB1 at a level of 50 μg AFB1 kg?1 and above can affect fish performance and disease resistance. Application of an effective mycotoxin management in the feed seems to be useful to prevent the negative effects of AFB1. 相似文献
6.
7.
8.
Kuehn CC Oliveira LG Santos CD Augusto MB Toldo MP do Prado JC 《Veterinary parasitology》2011,177(3-4):242-246
DHEA, a steroid hormone synthesized from cholesterol by cells of the adrenal cortex, plays an essential role in enhancing the host's resistance to different experimental infections. Receptors for this hormone can be found in distinct immune cells (especially macrophages) that are known to be the first line defense against Trypanosoma cruzi infection. These cells operate through an indirect pathway releasing nitric oxide (NO) and cytokines such TNF-α and IL-12 which in turn trigger an enhancement of natural killer cells and lymphocytes which finally secrete pro and anti-inflammatory cytokines. The effects of pre- and post-infection DHEA treatment on production of IL-12, TNFα and NO were evaluated. T. cruzi infected macrophages post treated with DHEA displayed enhanced concentrations of TNF-α, IL-12 and NO. Probably, the mechanisms that induced the production of cytokines by infected cells are more efficient when the immune system has been stimulated first by parasite invasion, suggesting that the protective role of DHEA is greater when administered post infection. 相似文献
9.
F. Miró R. Santos J. L. Garrido-Castro A. M. Galisteo R. Medina-Carnicer 《Veterinary research communications》2009,33(6):507-513
The handled trot of three Lusitano Purebred stallions was analyzed by using 2D and 3D kinematical analysis methods. Using
the same capture and analysis system, 2D and 3D data of some linear (stride length, maximal height of the hoof trajectories)
and angular (angular range of motion, inclination of bone segments) variables were obtained. A paired Student T-test was performed
in order to detect statistically significant differences between data resulting from the two methodologies With respect to
the angular variables, there were significant differences in scapula inclination, shoulder angle, cannon inclination and protraction-retraction
angle in the forelimb variables, but none of them were statistically different in the hind limb. Differences between the two
methods were found in most of the linear variables analyzed. 相似文献
10.