Thoracic high resolution CT using the modified VetMousetrap™ device is a feasible method for diagnosing canine idiopathic pulmonary fibrosis in awake West Highland White Terriers

Canine idiopathic pulmonary fibrosis is a chronic, progressive interstitial lung disease particularly prevalent in West Highland White Terriers. In the present prospective pilot study, we evaluated the feasibility of modified VetMousetrap? device in high resolution CT to detect idiopathic pulmonary fibrosis in West Highland White Terriers. Twelve awake West Highland White Terriers with canine idiopathic pulmonary fibrosis and 24 clinically healthy West Highland White Terriers were scanned using a helical dual slice scanner utilizing VetMousetrap? device without or with minimal chemical restraint with butorphanol. Three evaluators blindly assessed the images for image quality and the presence of canine idiopathic pulmonary fibrosis related imaging findings such as ground glass opacity and reticular opacities. Additionally, the attenuation of the lung was quantified with ImageJ software using histogram analysis of density over the lung fields. Computed tomography was successfully completed and motion artifact ranked in statistical analysis barely noticeable to mild in all dogs. The agreement between imaging findings and clinical status was very good with overall κ value 0.91 and percentage of agreement of 94%. There was also very good intraobserver (κ_range = 0.79‐0.91) and interobserver agreement (κ = 0.94). Moderate to severe ground glass opacity was present in all affected dogs. In the ImageJ analysis, a significant difference in lung attenuation between the study groups was observed. We conclude that modified VetMousetrap? device is applicable in diagnosing canine idiopathic pulmonary fibrosis in awake West Highland White Terriers avoiding anesthetic risk in these often severely hypoxic patients.     

The effects of a heat treatment on the behaviour of extractives in softwood studied by FTIR spectroscopic methods

Scots pine battens were heat-treated at 100–240º C under saturated steam. Cross-sections of heat-treated battens were analysed using ATR and reflection FTIR microscopies. A typical absorption band of fats and waxes at 1740 cm^-1 was detected on the sapwood edges in the temperature range of 100–160º C, indicating that fats and waxes moved along the axial parenchyma cells to the surface of the sapwood during the heat treatment. At the elevated temperatures (above 180° C) fats and waxes disappeared from the sapwood surface and were no longer detected with FTIR spectroscopy. Resin acids were detected at temperatures between 100 and 180º C in the middle of the battens. IR spectra of these spots showed a characteristic absorption band of resin acids at 1697 cm^-1. At 200º C resin acids were not detected in the middle of the battens; however, resin acids were detected at distances of 500 and 600 mm from the midpoint of the battens and on the edges of battens. At temperatures above 200° C, resin acids had disappeared from the wood.     

Fruit development in artificially self- and cross-pollinated strawberries (Fragaria × ananassa) and raspberries (Rubus idaeus)

Tunnel cultivation of berries is increasing on a global basis, and suitable management techniques are being studied for growing berry crops in a protected environment. Soil mulching and irrigation are among the techniques that significantly affect plant growth and yield. Tunnel cultivation is often limited to growing single cultivars, and little attention has been paid to the effects of growing several cultivars in the same tunnel. There are indications that crop yield and quality might benefit from cross-pollination. In this study, the effects of artificial self- and cross-pollination were studied in 15 strawberry and 6 red raspberry cultivars grown in a protected environment in a greenhouse. Four strawberry cultivars benefited from cross-pollination and one required it. In the case of raspberries, self- and cross-pollination had no effect on yield and fruit quality. In conclusion, particular berry cultivars are better suited to tunnel cultivation.     

Polycythemia in dogs with chronic hypoxic pulmonary disease

BackgroundProlonged tissue hypoxia caused by chronic pulmonary disease is commonly regarded as an important mechanism in the development of secondary polycythemia, but little clinical data are available to support this hypothesis.ObjectiveTo study the prevalence and severity of erythrocytosis accompanying chronic hypoxic pulmonary disease in dogs.AnimalsForty‐seven dogs with hypoxic chronic pulmonary disease, 27 dogs with nonhypoxic chronic pulmonary disease, and 60 healthy controls.MethodsDogs with chronic pulmonary disease and chronic hypoxemia (partial pressure of arterial oxygen [PaO₂] < 80 mm Hg on at least 2 arterial blood gas measurements a minimum of 1 month apart) were identified retrospectively from patient records. Association between arterial oxygen and red blood cell parameters was analyzed using Pearson''s correlation coefficients and multivariable linear regression analysis.ResultsRed blood cell parameters measured at the end of the hypoxemia period were within the laboratory reference range in most dogs. In chronically hypoxemic dogs, hematocrit (Hct) was increased in 4/47 (8.5%; 95% confidence interval [CI], 0‐17) dogs, erythrocyte count (Erytr) was increased in 12/47 (26%; 95%CI, 13‐38) dogs and hemoglobin concentration (Hb) was increased in 3/47 (6.4%; 95%CI, 0‐14) dogs. No marked polycythemia (Hct ≥65%) was noted in any of the dogs. Red blood cell parameters were not associated with the severity of hypoxemia (correlation to PaO₂: Erytr, r = −.14; Hb, r = −.21; Hct, r = −.14; P > .05 for all).Conclusions and Clinical ImportancePolycythemia is uncommon, and usually mild if present, in dogs with chronic hypoxia caused by pulmonary disease.     

Nitrate estimates using the Nitrachek Test for precise N-fertilization during plant growth and,after harvest,for quality testing potato tubers

Summary The effect of various levels, timing, and form of inorganic and organic nitrogenous fertilizers on the variation in nitrate content of stem bases over time was measured by means of the Nitrachek test in experiments carried out on sandy soils in Bremerv?rde, Germany (1985–7) and Helsinki, Finland (1987). There were good correlations between fertilizer use and nitrate content in the stem sap, and it was shown to be feasible to derive standard nitrate values which varied with growth stage. Splitting the application of N gave higher yields than applying all the N in the seedbed. The nitrate contents of tubers at harvest were correlated with the intensity of fertilizer use and was influenced by season. Soil N balances showed that splitting the N dressings reduced the soil N content more than applying all the nitrogen at the time of planting.     

Comparison of Conventional,Integrated and Organic Potato Production in Field Experiments in Finland

Abstract

In 1987, a potato research project called “Minimized use of chemical inputs in potato production” was started in all five Scandinavian countries. In this paper, the results of one field trial series carried out at the Potato Research Institute, Lammi, Finland, during the years 1987–90 are reported. The main plots in a split-plot designed trial series consisted of three cropping systems: conventional, integrated and organic. The subplots included three cultivars differing especially in their late blight (Phythophtora infestans, (Mont.) de Bary) resistance: Bintje, Record and Matilda.

Canopy measurements showed differences that can be attributed to different nitrogen supply in decreasing order from conventional to integrated to organic system. The trial sites were very heavily infested with potato scab, resulting in a very low percentage of I-class yield in susceptible cultivars Bintje and Matilda. Late blight was a serious problem in organically grown Bintje, as expected.

Total yields in the integrated and organic systems were 10% and 36% lower, respectively, than in the conventional system. There was an interaction between cropping system and cultivar in favour of Bintje and the conventional system and Record in the organic system. The percentage of I-class yield was lowest in the conventional system. Some of the quality characteristics were slightly improved in the integrated and/or organic systems. Storage losses, caused mainly by tuber blight, were high in organically grown potatoes.

There were no large differences in production costs between the cropping systems. The main determinants of the unit production cost of potatoes were total yield and yield of I-class potatoes. The average unit costs were 1.76 FIM kg^?1 in the conventional, 1.68 FIM kg^?1 in the integrated and 2.36 FIM kg^?1 in the organic system. Record showed the lowest unit production costs in all systems ?1.33, 1.37 and 1.80 FIM kg^?1, respectively.     

Genetic variation of pedunculate oak (Quercus robur L.) in the urban woodlands of Helsinki

We present a genetic profile of pedunculate oak (Quercus robur L.) in three woodlands of the Helsinki region. We genotyped all mature trees and samples of seedlings to evaluate patterns of genetic variation and gene flow, and evaluate the long-term stability and natural regeneration of these stands. Although five microsatellite loci showed no significant differences in heterozygosity between generations in most locations, significant (p?per se does not interfere with gene flow over the distances represented here. Based on our findings, a minimal-maintenance program reliant upon natural regeneration of oak in the public parks of Helsinki seems plausible.