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Rurick Salyaev Natalya Rekoslavskaya Anastasia Chepinoga Sergio Mapelli R. Pacovsky 《New Forests》2006,32(2):211-229
The transgenic poplar (Populus tremula L.) was obtained by transfer of the ugt and acb genes via triparental mating, which was employed to deliver large fragments of TDNA as a cluster. Freshly harvested seeds
of local poplar were placed on MS agar medium and plantlets were obtained. After 1 year of subcultivation, plantlets were
infected with a transconjugant of triparental mating with target ugt and acb genes into axillary buds. The transformed sprouts so obtained were cut and subcultivated on agar medium with an addition
of 0.6 mg/l indole-3-butyric acid as an auxin source. The transformed sprouts showed GUS activity and resistance to gentamycin
and kanamycin. The integrity of the target ugt and acb genes into poplar genome was demonstrated via PCR and Southern blot hybridisation. The transgenic poplar plants revealed
a higher growth energy, corresponding to a higher content of IAA as opposed to control plants. Both transgenic and non-transformed
plants were potted into soil for outdoor acclimatisation and subsequently transferred to earth in beds. Growing outside during
3 years, the transgenic poplar demonstrated a higher growth rate with fast bud and branch development. 相似文献
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