Biochemical Changes during Post-larval Growth in White Muscle of Gilthead Sea Bream (Sparus aurata) fed Zinc-fortified Diets

Veterinary Research Communications -     

Control of Fusarium Wilt of Radish by Combining Pseudomonas putida Strains that have Different Disease-Suppressive Mechanisms

ABSTRACT Biological control of soilborne plant pathogens in the field has given variable results. By combining specific strains of microorganisms, multiple traits antagonizing the pathogen can be combined and this may result in a higher level of protection. Pseudomonas putida WCS358 suppresses Fusarium wilt of radish by effectively competing for iron through the production of its pseudobactin siderophore. However, in some bioassays pseudobactin-negative mutants of WCS358 also suppressed disease to the same extent as WCS358, suggesting that an, as yet unknown, additional mechanism may be operative in this strain. P. putida strain RE8 induced systemic resistance against fusarium wilt. When WCS358 and RE8 were mixed through soil together, disease suppression was significantly enhanced to approximately 50% as compared to the 30% reduction for the single strain treatments. Moreover, when one strain failed to suppress disease in the single application, the combination still resulted in disease control. The enhanced disease suppression by the combination of P. putida strains WCS358 and RE8 is most likely the result of the combination of their different disease-suppressive mechanisms. These results demonstrate that combining biocontrol strains can lead to more effective, or at least, more reliable biocontrol of fusarium wilt of radish.     

Tomato early blight (Alternaria solani): the pathogen, genetics, and breeding for resistance

Alternaria solani causes diseases on foliage (early blight), basal stems of seedlings (collar rot), stems of adult plants (stem lesions), and fruits (fruit rot) of tomato. Early blight is the most destructive of these diseases and hence receives considerable attention in breeding. For over 60 years, breeding for early blight resistance has been practiced, but the development of cultivars with high levels of resistance has been hampered by the lack of sources of strong resistance in the cultivated tomato and by the quantitative expression and polygenic inheritance of the resistance. In some accessions of wild species, high levels of early blight resistance have been found, but breeding lines still have unfavorable horticultural traits from the donor parent. Recently, the first linkage maps with loci controlling early blight resistance have been developed based on interspecific crosses. These maps may facilitate marker-assisted selection. This overview presents the current knowledge about the A. solani–tomato complex with respect to its biology, genetics, and breeding.     

Exotic tree species displace indigenous ones on farms at intermediate altitudes around Mount Kenya

Agroforestry systems are potentially suitable for conservation of tree genetic resources. Farmers around Mt. Kenya usually integrate trees into their farm. Large parts of these trees seem to be of exotic origin, whereas indigenous species have priority for conservation. This study aimed at determining on-farm richness, composition and frequency of indigenous and exotic woody species around Mount Kenya to assess the suitability of farms for the conservation of indigenous tree species. 265 on-farm plots of 0.5 ha size each were selected in 18 different agro-ecological zones by using a stratified sampling scheme. All woody species within the plot were recorded with their local and scientific names. Total species richness was 424 (including 306 indigenous ones), mean richness per plot 16.5 species (including 8.8 indigenous ones). Eight out of the 10 most frequent species were exotic ones with Grevillea robusta from Australia ranking first (found on almost 76% of the surveyed farms). The proportion of indigenous species increased with increasing aridity and temperature. Dominance of exotic species was found at farms of humid mid- and highlands. Ordination analysis revealed that mostly exotic species contributed to separation of farms in the highlands and upper midlands, whereas indigenous species in the lower midlands and lowlands. As the frequencies of most indigenous trees were low, only parts of the surveyed farms can contribute to conservation of tree genetic resources, particularly the less intensively managed farms of the more arid lands. Farmers’ access to knowledge on valuable indigenous tree species and to quality seedlings of these trees need to be improved to increase indigenous species’ frequencies on farms and possibly to replace some of the exotic species in the future.     

Opportunities for domesticating the African baobab (Adansonia digitata L.): multi-trait fruit selection

Among the priority species identified for domestication in the Sahel region of Sub-Sahara Africa is the baobab (Adansonia digitata L.) tree. The quantification of the variation in nutrient content and fruit morphological traits of trees is one of the most important steps in identifying superior planting material for domestication. Ten provenances in Mali covering all the different agro-ecological zones were selected and the fruit morphological traits, vitamin C, calcium, iron and colour were studied. Mean pulp content was 2,149 ± 1,117 , 2,406 ± 776  and 25 ± 17 mg kg^?1 for vitamin C, calcium and iron, respectively. Fruit pulp colour varied from white, creamy to pink and a significant correlation between pulp vitamin C content and reflectance in the green and blue bands was observed. Significant negative correlations were found between rainfall and pulp vitamin C content and between mean annual temperature and fruit and pulp weight and pulp fraction, suggesting that these traits are influenced by the environment. Pulp iron content correlated positively with topsoil sodicity and base saturation. Similarly, pulp vitamin C content correlated positively with topsoil sand fraction. Pulp reflectance in the blue and green bands correlated negatively with topsoil pH water and base saturation, respectively. The variation in nutritive and morphological traits offers the opportunity for selecting plus trees with a combination of desired traits for domestication.     

Evaluation of frozen Umbrella‐stage Artemia as first animal live food for Litopenaeus vannamei (Boone) larvae

An alternative larval shrimp feeding regime, in which umbrella‐stage Artemia were constituting the first zooplankton source was evaluated in the culture of Litopenaeus vannamei. In a preliminary experiment, umbrella‐stage Artemia were fed to larvae from zoea 2 (Z2) to mysis 2 (M2) stages to identify the larval stage at which raptorial feeding starts and to determine daily feeding rates. The following experiment evaluated the performance of two feeding regimen that differed during the late zoea/early mysis stages: a control treatment with frozen Artemia nauplii (FAN), and a treatment with frozen umbrella‐stage Artemia (FUA). The ingestion rate of umbrella‐stage Artemia increased from nine umbrella per larvae day^?1 at Z2 stage to 21 umbrella per larvae day^?1 at M2. A steep increase in ingestion and dry weight from Z3 to M2 suggests a shift to a raptorial feeding mode at the M1 stage. Treatment FUA exhibited a significantly higher larval stage index (P < 0.05) during the period that zoea larvae metamorphosed to the mysis stage, and a higher final biomass, compared with treatment FAN. Based on these results and on practical considerations, a feeding regime starting with umbrella‐stage Artemia from Z2 sub‐stage can be recommended for L. vannamei larvae rearing.     

竹叶叶绿素荧光季节变化的模型设计

在寻找可再生能源和生物基材料方面,竹子已经被作为一种高产的生物质产品,可以用来作为土壤污染的修复性植物。由于这个原因,一个产量指示的增长模型需要一个健康的指标参数来检测植物的性能。因此,本研究对位于爱尔兰都伯林的Ballyboughalo公司的毛竹蒿草(Phyllostachys humilis)叶子的叶绿素荧光进行了测量。本研究测量结果涵盖了这种竹类每个节点的叶子,并且建立在了随季节性变化的基础上。最常使用的叶绿素荧光参数是Fv/Fm值,又称TRO/ABS (即被困的光子与吸收的光子数之比)。随季节的推移以及这个参数较大的变化,本研究将春天与一年中其它的季节进行对比观察测量。在过去的一年,这种植物的上部叶片比下部叶片表现更好。本研究的发现被整合在一个数学模型中,其目的是为预测和解释在其它立地条件下的植物的性能。     

Foliar Nitrogen Uptake from Wet Deposition and the Relation with Leaf Wettability and Water Storage Capacity

This study assessed the foliar uptake of ¹⁵N-labelled nitrogen (N) originating from wet deposition along with leaf surface conditions, measured by wettability and water storage capacity. Foliar ¹⁵N uptake was measured on saplings of silver birch, European beech, pedunculate oak and Scots pine and the effect of nitrogen form (NH ₄ ⁺ or NO ₃ ^? ), NH ₄ ⁺ to NO ₃ ^? ratio and leaf phenology on this N uptake was assessed. Next to this, leaf wettability and water storage capacity were determined for each tree species and phenological stage, and the relationship with ¹⁵NH ₄ ⁺ and ¹⁵NO ₃ ^? uptake was examined. Uptake rates were on average five times higher (p?<?0.05) for NH ₄ ⁺ than for NO ₃ ^? and four times higher for deciduous species than for Scots pine. Developing leaves showed lower uptake than fully developed and senescent leaves, but this effect was tree species dependent. The applied NH ₄ ⁺ to NO ₃ ^? ratio did only affect the amount of N uptake by senescent leaves. The negative correlation between measured leaf contact angles and foliar N uptake demonstrates that the observed effects of tree species and phenological stage are related to differences in leaf wettability and not to water storage capacity.