番鸭血液生化指标与产蛋性能的关系

采用托蛋法对番鸭进行个体产蛋量测定 ,并于 5 6周龄时根据其产蛋量高、中、低分为 3组 ,每组 2 0只 ,对其 9项血液生化指标进行测定 ,探讨有关生化指标与产蛋性能之间的关系 ,为番鸭早期选种提供科学依据 .结果表明 :(1)谷草转氨酶、碱性磷酸酶、乳酸脱氢酶活性及甘油三酯、总胆固醇和低密度脂蛋白胆固醇含量与产蛋性状均呈弱相关 (r=0 .0 0 8- 0 .2 10 ) ;(2 )谷丙转氨酶活性与开产日龄呈极显著负相关 (p<0 .0 1) ,与开产体重呈弱负相关 (p>0 .0 5 ) ,与 4 3周龄和 5 6周龄产蛋量均呈极显著正相关 (p <0 .0 1) ;(3)血清总蛋白含量与5 6周龄产蛋量呈显著正相关 (p<0 .0 5 ) ,与其它产蛋性状无显著相关 (p>0 .0 5 ) ;(4 )高密度脂蛋白胆固醇含量与开产日龄呈极显著正相关 (p<0 .0 1) ,与开产体重呈显著正相关 (p<0 .0 5 ) ,与 4 3周龄和 5 6周龄产蛋量均呈极显著负相关 (p<0 .0 1) .     

黑番鸭VIPR-1基因单核苷酸多态性与就巢性状的关联性分析

VIPR\|1基因是研究家禽就巢性状的重要候选基因,研究选用处在产蛋期、就巢期的黑番鸭为研究对象,通过PCR\|RFLP和测序的方法,对VIPR\|1基因内含子12进行多态性检测,并分析其与就巢性状之间的关系。结果表明：VIPR\|1基因内含子12存在一个AfaⅠ酶切位点,该位点在内含子12的902 bp处发生了C→T的碱基突变,产生了CC,CT和TT三种基因型。通过对产蛋黑番鸭与就巢黑番鸭个体的研究,发现所测产蛋黑番鸭基因型的分布情况为CC（32）,TT（40）和CT（42）,就巢黑番鸭基因型的分布为CC（18）,TT（40）和CT（18）,经卡方独立性检验,产蛋黑番鸭与就巢黑番鸭两者之间基因型的分布差异显著（P＜005）。表明黑番鸭VIPR\|1基因第12内含子C902T突变与黑番鸭的就巢性状显著相关。     

氰戊菊酯在植烟土壤中的降解

为了评价氰戊菊酯在烟田使用后的生态环境行为与效应,采用室内模拟方法,借助气相色谱分析技术研究了氰戊菊酯在6种植烟土壤(分别来自湖南湘西、云南保山、广东南雄、河南三门峡、福建光泽、山东泰安)中的降解半衰期(T1/2)与土壤理化性状的关系.结果表明:氰戊菊酯在河南三门峡的植烟土壤中降解最快,T1/2为8.27 d,而在福建光泽的植烟土壤中降解最慢,T1/2为12.62 d;随着植烟土壤中有机质含量的增加,氰戊菊酯的降解半衰期延长;在pH值为6.35～6.60的植烟土壤中,氰戊菊酯降解半衰期较短,在此范围外,随着pH值的升高或降低半衰期均有延长趋势;氰戊菊酯降解半衰期与植烟土壤粘粒含量的关系较为复杂,粘粒含量低于180 g/kg时以物理吸附为主,大于180 g/kg时以化学吸附为主.     

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Effect of replacements of fishmeal and fish oil on growth and dioxin contents of rainbow trout

Eight experimental diets were prepared to determine the effect of replacements of fishmeal and fish oil with plant protein and palm oil on the growth and dioxin contents in rainbow trout. The first four diets (FP0, FP5, FP10, and FP15) included fishmeal (55%) with increasing replacement of fish oil by palm oil (0, 5, 10, and 15%). Four other diets (AP0, AP5, AP10, and AP15) were substituted with an alternative protein source and with increasing palm oil content (0, 5, 10, and 15%). Dietary dioxin contents increased as fish oil level increased. Thirty rainbow trout (mean weight 38 g) were fed the experimental diets for 12 weeks. No significant differences in growth performance and feed utilization were observed. Dietary dioxin contents influenced the dioxin contents in the whole body. These results suggest that palm oil could effectively replace fish oil in rainbow trout diets, and reduce the dioxin contents in fish.     

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农用地分等定级与估价信息系统（ALEIS）研制

根据农用地分等定级与估价的基本原理与方法 ,运用组件式地理信息系统 (GIS)和计算机技术 ,研制开发了适于中国国情的通用农地分等定级与估价信息系统 (AL EIS)雏型。该系统包括文件、视图、图层、对象、布局、数据库、分等定级与估价、窗口和帮助等 9大模块 ,具有属性数据库管理、空间数据库管理、农地分等定级与估价、以及成果输出等方面的功能 ,且界面友好 ,操作简便 ,可实现农用土地分等定级与估价自动化 ,为各级土地部门和政府部门提供界面友好和便捷适用的计算机业务管理工具。     

水稻偏籼型广亲和恢复系T2070的选育

通过人工杂交 ,将广亲和基因和胞质雄性不育恢复基因重组 ,选育出偏籼型广亲和强恢复系 T2 0 70。经与籼型三系不育系配组鉴定 ,其组合表现为杂种优势强、穗大粒多、耐肥抗倒、丰产性好。配制的 优 2 0 70组合已于 1 999年 3月通过浙江省品种审定。     

Differential protein profiles in duck meat during the early postmortem storage period

The present study was designed to investigate proteomic differences in duck breast muscle during the early postmortem storage period. The meat quality was evaluated at 0 hr and 24 hr postmortem at 4°C in Pekin ducks, black Muscovy ducks and Mule ducks. Differentially expressed proteins were detected by two‐dimensional gel electrophoresis (2‐DE) and matrix‐assisted laser desorption ionization‐time‐of‐flight mass spectrometry (MALDI‐TOF/TOF MS) at 0 hr and 24 hr postmortem in the three duck breeds. The results showed that 53 proteins spots were differentially expressed at 0 hr and 24 hr postmortem at 4°C in Pekin ducks, 75 spots in black Muscovy ducks, and 72 spots in Mule ducks. A total of 30 (10 spots for each breed) were selected for identification by mass spectrometry. Seven proteins were identified in Pekin ducks, eight in black Muscovy ducks and seven in Mule ducks. Moreover, the above results obtained by 2‐DE and MALDI‐TOF/TOF MS were confirmed by western blotting. To our knowledge, this study is the first to provide insights into the protein profiles of ducks during postmortem storage and provides a better understanding of the biochemical processes that contribute to duck meat quality.