Evaluation of environmental functions of tropical forest in Kinabalu Park, Sabah, Malaysia using GIS and remote sensing techniques: Implications to forest conservation planning

Environmental functions of tropical forest can serve as criteria for forest conservation planning in the tropics. The objective of this study is to evaluate the environmental functions of tropical forest in Kinabalu Park, Sabah, Malaysia, using GIS and remote sensing techniques. Field data, statistical data, including weather data with geographic localities, maps and satellite image are collected. Linear regression models are developed for forests of different geological substrates, based on the relationships between altitude and biodiversity (Fisher’s alpha index). Biodiversity conservation function map is derived with the statistical models and a digital elevation model. Coupling with extensive literature review, an evaluation matrix for evaluating soil and water conservation functions including landslide prevention, flood prevention and drought prevention functions, is constructed. To evaluate the soil and water conservation functions, a weighted linear combination method is used with GIS layers of topography, geology, soil depth, rainfall and slope. Forest areas in Kinabalu Park are derived with land cover mapping using Landsat-TM image. Areas having high values of biodiversity conservation, flood and drought prevention functions are covered with mainly lowland rain forest. On the other hand, areas with high values of the landslide prevention function are covered with mainly subalpine forests. Using the environmental functions, a conservation index is computed to represent forests that are important to conservation. Based on theCI, the lowland rain forest receives highest priority in protection. In fact, it is located in the boundary areas of the park and thus exposed to illegal activities. This study would not be possibly accomplished without the map data as well as other data.     

Distribution of prion protein genotypes in breeds of sheep in New Zealand

AIM: To use an established high through-put genotyping procedure to gain an estimate of the frequency of alleles of the prion protein (PrP) gene in some common sheep breeds in New Zealand. METHODS: Using a genotyping procedure based on matrix-assisted laser desorption ionisation-time of flight (MALDI-TOF), DNA samples from 3,024 sheep from New Zealand, including breeds such as Romney, Texel, Coopworth, Merino and mixed breed, were isolated, genotyped and the results analysed. RESULTS: The 15 scrapie genotypes commonly reported, and derived from the five commonly reported allelic variants (ARR, ARQ, AHQ, ARH and VRQ), were all observed in the samples analysed. The estimates were indicative of the frequencies in the population of alleles present in breeds of sheep in New Zealand. There was a significant difference between the frequencies of alleles between breeds, but the ARQ, followed by the ARR allele, were, except in Carwell sheep, the most common alleles present. CONCLUSION: This study gave an indication of the percentages of PrP gene alleles in sheep in New Zealand, including data previously unreported from breeds in this country. It is of interest because of the relatively large size of the sheep population in New Zealand compared with many countries, and it provides some useful information on the genetic susceptibility or resistance of the sheep population in New Zealand to scrapie. The frequencies of the alleles can be different for an individual breed compared between countries.     

Inheritance of resistance to facial eczema: a review of research findings from sheep and cattle in New Zealand

Facial eczema (FE) is a costly problem to New Zealand pastoral agriculture, and has a detrimental impact on animal wellbeing. Incidence and severity of the disease can be reduced by grazing management and zinc prophylaxis. An additional strategy is to breed animals that are genetically resistant to intoxication with sporidesmin, the causative mycotoxin. This review summarises research findings on the inheritance of resistance of animals to FE, including evidence of among- and within-breed genetic variation, direct and correlated responses to selection, and identification of genetic markers and candidate genes for FE resistance.     

A PCR-based sex determination method for possible application in caprine gender selection by simultaneous amplification of the Sry and Aml-X genes

Sex determination of livestock is performed to achieve the objectives of livestock breeding programmes. Techniques for sex determination have evolved from karyotyping to detecting Y-specific antigens and recently to the polymerase chain reaction (PCR), which appears to be the most sensitive, accurate, rapid and reliable method to date. In this study, a PCR-based sex determination method for potential application in goat breeding programmes was developed. Primers were designed to amplify a portion of the X amelogenin gene (Aml-X) on the X chromosome to give a 300 bp product and Sry gene on the Y chromosome to give a 116 bp product. PCR optimization was performed using DNA template extracted from a whole blood sample of Jermasia goats (German Fawn x Katjang) of both sexes. It was possible to identify the sex chromosomes by amplifying both male- and female-specific genes simultaneously in a duplex reaction with males yielding two bands and females yielding one band. The Aml-X primer set, which served as an internal control primer, did not interfere with amplification of the Y-specific sequence even when a low amount of DNA (1 ng) was used. The duplex reaction subjected to a blind test showed 100% (14/14) concordance, proving its accuracy and reliability. The primer sets used were found to be highly specific and were suitable for gender selection of goats.