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Inokuma H Ohashi M Jilintai Tanabe S Miyahara K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(6):661-664
DNA from 111 ticks collected by flagging in Tokachi district, Eastern Hokkaido, Japan were examined for infection with Rickettsia and Ehrlichia, by PCR and sequencing methodology. For Rickettsia, analysis of the partial sequence of the citrate synthase gene was successfully performed on 11 DNA samples from I. persulcatus, and 7 of them showed 99.8% identical with Rickettsia helvetica while the other 4 showed 99.8% identical with ;Candidatus Rickettsia tarasevichiae'. For Ehrlichia, a partial sequence of the 16S rRNA gene detected from I. persulcatus was 100% identical with that from Ehrlichia muris, and another DNA sample from I. ovatus showed 99.8% identical with Ehrlichia species detected from I. ovatus. The results suggest that the pathogens detected here might be distributed in this area. 相似文献
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Cerebellar ataxia due to Toxocara infection in Mongolian gerbils,Meriones unguiculatus 总被引:3,自引:0,他引:3
Akao N Tomoda M Hayashi E Suzuki R Shimizu-Suganuma M Shichinohe K Fujita K 《Veterinary parasitology》2003,113(3-4):229-237
We assessed the usefulness of gerbils as an experimental model for neurologic toxocarosis. Mongolian gerbils, Meriones unguiculatus, infected with Toxocara canis or Toxocara cati (1000 eggs/gerbil) showed progressive neurologic disorders from 50 days after infection in T. canis-infected gerbils or from 120 days after infection in T. cati-infected gerbils. The incidence of the onset was 6 of the 13 gerbils (49%) in the T. canis-gerbils and 5 of the 7 gerbils (71%) in the T. cati-gerbils. Histopathologically, the cerebellum was the most affected in both groups. We observed loss of Purkinje cells, glial nerve fibers, and nerve sheaths. We also found foci consisting of aggregated macrophages scattered in the white matter of the cerebellum. The affected gerbils showed ataxia and ultimately died of cachexia. Our findings suggest that irreversible neurologic toxocarosis in gerbils can be induced by infection with either T. canis or T. cati. 相似文献
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As a measure of estimating humidity control capacity of materials in an airtight room under sinusoidal temperature variation,
we used the Cb value, which is the ratio of the range of variation in relative humidity in a steel box lined with the material of interest
to the range in an empty steel box. In order to clarify the factors that affect the Cb value, we focused on both the temperature variation rate and the area that is lined in the box. It was found that changing
the rate of temperature variation over one period strongly affected the Cb value, although the amplitude of temperature variation did not. We further noticed that the difference between the time when
peak temperature was reached and the time when peak absolute humidity was reached (peak time difference), was found to be
useful for estimating the humidity control capacity of materials. Knowing the difference between the phase when peak temperature
was reached and the phase when peak absolute humidity was reached (phase angle difference) was also useful. Because the Cb value was affected by both the variation period and the lined area in the box, we can draw a contour diagram of the period
and the lined area for materials to give an overview of the humidity control capacity of a material. The materials of primary
interest in this study were Japanese cedar and porous ceramics. 相似文献
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Dielectric properties in three main directions for hinoki wood (Chamaecyparis obtusa) specimens conditioned at various levels of relative humidity were measured in the frequency range from 20 Hz to 10 MHz over
the temperature range from −150°C to 20°C. Three relaxations were observed in the specimens conditioned at high levels of
relative humidity. The relaxation in the highest frequency range was ascribed to the motions of adsorbed water molecules.
The relaxation in the middle frequency range remained unchanged by the ethanol–benzene extraction of specimens. The relaxation
location was independent of measuring directions. The relaxation in the lowest frequency range was not detected in the specimens
impregnated with methyl methacrylate (MMA). This result suggested that the relaxation was due to electrode polarization. The
Cole-Cole circular arc law applied well to two relaxations recognized in the specimens impregnated with MMA. The relaxation
magnitude in the middle frequency range was extremely large, and the distribution of relaxation times was very narrow. These
characteristics suggested relaxation of the Maxwell-Wagner type resulting from the interfacial polarization in the heterogeneous
structure, which included adsorbed water with large electrical conductivity within the insulating cell walls. 相似文献
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Kaneda T Shimizu K Urakawa N Nakajyo S 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(9):1047-1052
Effects of various selective phosphodiesterase (PDE) inhibitors on muscle contractility and cyclic nucleotide contents in guinea pig taenia coli were investigated. Forskolin and sodium nitroprusside inhibited carbachol (CCh)-induced contraction in a concentration-dependent manner. Various selective PDE inhibitors, vinpocetine (type 1), erythro -9-(2-hydroxy-3-nonyl)adenine (EHNA, type 2), milrinone (type 3), Ro20-1724(type 4) and zaprinast (type 5) inhibited CCh-induced contraction in a concentration-dependent manner, but the inhibition of milrinone was noticeably smaller than that of the other PDE inhibitors. The rank order of potency was zaprinast > vinpocetine > EHNA > Ro20-1724 > milrinone. In the presence of CCh (0.3 microM), vinpocetine and Ro20-1724 both increased cAMP content, but not cGMP. By contrast, EHNA and zaprinast both increased cGMP content, but not cAMP. Pretreatment with ODQ (30 microM), a soluble guanylyl cyclase inhibitor, decreased the inhibition of CCh-induced contraction by EHNA or zaprinast. Pretreatment with SQ22536 (100 microM), an adenylyl cyclase inhibitor, decreased the inhibition of CCh-induced contraction by vinpocetine or Ro20-1724. In conclusion, it was indicated that vinpocetine- or Ro20-1724-induced relaxation was correlated with cAMP but EHNA- or zaprinast- induced relaxation was correlated with cGMP. 相似文献
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Shibata SJ Hiramatsu Y Kaseda M Chosa M Ichihara N Amasaki H Hayakawa T Asari M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(11):1143-1147
Lymph drainage routes from the abdominal and pelvic cavities in beagle dogs were observed serially by following the time course of India ink administered intraperitoneally. Four systems of lymph drainage routes from the peritoneal cavity were observed in this study. The earliest drainage returned to the cranial mediastinal lymph nodes via the sternal lymph vessels; subsequently, the sternal lymph nodes located along the internal thoracic artery became involved. Then, a drainage route via the lymph vessel along the left vagus nerve was observed. The final drainage route flowed into the lateral lymph vessel through the thoracic duct located on the vertebra. These results show that India ink is absorbed from the peritoneal cavity, and that the lymph drainage first flows mainly towards the cranial mediastinal lymph nodes through the ventral lymphatic channels. Our serial observations suggest that, over time, the lymph drainage routes changed from the ventral abdominal to the dorsal thoracic lymphatic channels in the thorax. 相似文献
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Ruenphet S Satoh K Tsujimura M Hasegawa T Takehara K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(7):905-908
Strategies of Newcastle disease (ND) vaccination were demonstrated in a commercial ostrich farm in Japan. Three of 13 seven-month-old ostriches kept in a pen were vaccinated with a live ND vaccine by eye dropping for the 1st and 2nd vaccinations and spraying for the 3rd to 5th vaccinations. Antibodies against ND virus (NDV) were detected in all of the unvaccinated ostriches by virus neutralization test. At 2.5 months post final vaccination, 2 ostriches introduced into the pen raised antibodies against NDV. These data indicate that NDV may be transmitted from vaccinated to unvaccinated ostriches in the flock and that the virus may be sustained for a certain period in the flock. These data may be helpful for ND vaccination management in ostrich farms. 相似文献
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Imai K Yamagishi N Okura N Fukuda T Hirata T Okada K Sato S Furuhama K 《Veterinary journal (London, England : 1997)》2012,193(1):174-179
To develop a simple procedure for estimating glomerular filtration rate (GFR) in calves, a three-sample method using iodixanol was first compared to that using the standard agent inulin. Iodixanol and inulin were co-administered intravenously to calves at 40 mg I/kg and 40 mg/kg, respectively, and blood was collected 30, 60, 120, and 180 min later. Serum iodixanol and inulin concentrations were separately determined by high performance liquid chromatography and colorimetry. Serum urea nitrogen (UN) and creatinine concentrations were also measured. GFR estimated by iodixanol was consistent with that using inulin in clinically healthy calves. Based on GFR estimations in healthy calves and those renal-loaded with iodixanol, it was found that the serum creatinine concentrations became elevated when GFR decreased to 60% of the reference value. In contrast, serum UN concentrations fluctuated widely, presumably due to extra-renal factors. When GFR was estimated using the three-sample method and compared with the single-blood-sample method, 62/69 (90%) of samples tested were within the agreement plots. The results demonstrated that the single-blood-sample method using iodixanol may be useful in monitoring GFR in calves. 相似文献
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