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排序方式: 共有674条查询结果,搜索用时 15 毫秒
1.
Radiocarbon dates associated with engraved stones from sealed archeological deposits at Wonderwerk Cave in the northern Cape Province indicate that rock engraving in South Africa is at least 10,000 years old. 相似文献
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Expression of porcine endogenous retroviruses (PERVs) in melanomas of Munich miniature swine (MMS) Troll 总被引:1,自引:0,他引:1
Dieckhoff B Puhlmann J Büscher K Hafner-Marx A Herbach N Bannert N Büttner M Wanke R Kurth R Denner J 《Veterinary microbiology》2007,123(1-3):53-68
Porcine endogenous retroviruses (PERVs) are integrated in the genome of all pig breeds. Since some of them are able to infect human cells, they might represent a risk for xenotransplantation using pig cells or organs. However, the expression and biological role of PERVs in healthy pigs as well as in porcine tumours is largely unknown. Since we and others have recently shown overexpression of a human endogenous retrovirus, HERV-K, in human melanomas, we studied the expression of PERVs in melanomas of selectively bred Munich miniature swine (MMS) Troll. This breeding herd of MMS Troll is characterised by a high prevalence of melanomas, which histologically resemble various types of cutaneous melanomas in humans. Several genetic factors have been defined when studying inheritance of melanomas and melanocytic nevi in MMS Troll. Here we show that the polytropic PERV-A and PERV-B as well as the ecotropic PERV-C are present in the genome of all melanoma bearing MMS Troll investigated. Most interestingly, in the spleen, but not in other organs, recombinant PERV-A/C proviruses were found. PERV expression was found elevated in melanomas when compared to normal skin and viral proteins were expressed in melanomas and pulmonary metastasis-derived melanoma cell cultures. During passaging of these cells in vitro the expression of PERV mRNA and protein increased and virus particles were released as shown by RT activity in the supernatant and by electron microscopy. Genomic RNA of PERV-A, -B and -C were found in pelleted virus particles. Although PERV expression was elevated in melanomas and pulmonary metastasis-derived cell cultures, the function of the virus in tumour development is still unclear. 相似文献
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Fernanda S. ORREGO Mathias HÜNE Hugo A. BENÍTEZ Mauricio F. LANDAETA 《Integrative zoology》2023,18(2):372-384
Phenotypic variation in organisms depends on the genotype and the environmental constraints of the habitat that they exploit. Therefore, for marine species inhabiting contrasting aquatic conditions, it is expected to find covariation between the shape and its spatial distribution. We studied the morphology of the head and cephalic sensory canals of the eelpout Austrolycus depressiceps (4.5–22.5 cm TL) across its latitudinal distribution in South Pacific (45°S–55°S). Geometric morphometric analyses show that the shape varied from individuals with larger snout and an extended suborbital canal to individuals with shorter snouts and frontally compressed suborbital canal. There was size variation across the sampled populations, but that size does not have a clear latitudinal gradient. Only 8% of the shape variation relates to this size variation (allometry), represented by a decrease in the relative size of the eye, and a depression of the posterior margin of the head. There were spatial differences in the shape of the head, but these differences were probably caused by allometric effects. Additionally, 2 of the canals of the cephalic lateralis pores and the head shape showed modularity in its development. This study shows that the morphology of marine fish with a shallow distribution varies across distances of hundreds of kilometers (i.e., phenotypic modulation). 相似文献
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Paul C. Stoy Mathew Williams Mathias Disney Ana Prieto-Blanco Brian Huntley Robert Baxter Philip Lewis 《Landscape Ecology》2009,24(7):971-986
Transferring ecological information across scale often involves spatial aggregation, which alters information content and
may bias estimates if the scaling process is nonlinear. Here, a potential solution, the preservation of the information content
of fine-scale measurements, is highlighted using modeled net ecosystem exchange (NEE) of an Arctic tundra landscape as an
example. The variance of aggregated normalized difference vegetation index (NDVI), measured from an airborne platform, decreased
linearly with log(scale), resulting in a linear relationship between log(scale) and the scale-wise modeled NEE estimate. Preserving
three units of information, the mean, variance and skewness of fine-scale NDVI observations, resulted in upscaled NEE estimates
that deviated less than 4% from the fine-scale estimate. Preserving only the mean and variance resulted in nearly 23% NEE
bias, and preserving only the mean resulted in larger error and a change in sign from CO2 sink to source. Compressing NDVI maps by 70–75% using wavelet thresholding with the Haar and Coiflet basis functions resulted
in 13% NEE bias across the study domain. Applying unique scale-dependent transfer functions between NDVI and leaf area index
(LAI) decreased, but did not remove, bias in modeled flux in a smaller expanse using handheld NDVI observations. Quantifying
the parameters of statistical distributions to preserve ecological information reduces bias when upscaling and makes possible
spatial data assimilation to further reduce errors in estimates of ecological processes across scale. 相似文献
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Mathias?FosuEmail author Ronald?F.?Kühne Paul?L.?G.?Vlek 《Biology and Fertility of Soils》2003,39(2):117-122
In order to understand the efficiency of residue-N use and to estimate the minimum input required to obtain a reasonable level of crop response, it is important to quantify the fate of the applied organic-N. The recovery of N from 15N-labelled Crotalaria juncea was followed in the soil and the succeeding maize crop. Apparent N recovery (ANR) by maize from unlabelled Crotalaria juncea, Crotalaria retusa, Calopogonium mucunoides, Mucuna pruriens and mineral fertilizer at three locations were also evaluated. The maize crop recovered 4.7% and 7.3% of the 15N-labelled C. juncea-N at 42 days after sowing (DAS) and at final harvest, respectively. The corresponding 15N recovery from the soil was 92.4% and 58.5%. The highest mean ANR of 57.4% was with mineral fertilizer, whereas the mean ANR of 14.3% from C. retusa was the lowest. A large pool substitution and added-N interaction effect was observed when comparing N recovery from the labelled and unlabelled C. juncea. The amount of residue-N accounted for by the isotope dilution method at 42 DAS was 97.1% and at final harvest 65.8%. The large residue-N recovery in the soil organic-N pool explains the residual effect usually observed with organic residue application. 相似文献
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Changes in phosphorus fractions in three tropical soils amended with corn cob and rice husk biochars
Joseph Osafo Eduah Mark Kofi Abekoe Mathias Neumann Andersen 《Communications in Soil Science and Plant Analysis》2020,51(10):1331-1340
ABSTRACT The formation of phosphorus (P) compounds including iron-P, aluminum-P and calcium-P in highly weathered tropical soils can be altered upon biochar addition. We investigated the effect of corn cob biochar (CC) and rice husk biochar (RH) pyrolyzed at three temperatures (300°C, 450°C and 650°C) on phosphorus (P) fractions of three contrasting soils. A 90d incubation study was conducted by mixing biochar with soil at a rate of 1% w/w and at 70% field capacity. Sequential P fraction was performed on biochar, soil and soil-biochar mixtures. Increase in most labile P (resin-Pi, NaHCO3-Pi) and organic P fraction (NaHCO3-Po + NaOH-Po) in CC and RH biochars were inversely related to increasing temperature. HCl-Pi and residual P increased with increasing temperature. Interaction of CC and RH with soils resulted in an increase in most labile P as well as moderately labile P (NaOH-Pi) fractions in the soils. CC increased most labile P in the soils more than RH. The increase in most labile P fraction in soils was more significant at relatively lower temperatures (300°C and 450°C) than 650°C. However, the increase in HCl-Pi and residual P of the soils was more predominant at high temperature (650°C). The study suggested that biochar pyrolyzed at 300–450°C could be used to increase P bioavailability in tropical soils. 相似文献
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