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Madhugiri Nageswara-Rao Micaha Hanson Sujata Agarwal C. Neal Stewart Jr. Charles Kwit 《Agroforestry Systems》2014,88(5):823-834
The agricultural landscape of the United States could soon be changed by planting of switchgrass (Panicum virgatum L.) cultivars to meet government-mandated targets for lignocellulosic bioenergy production and consumption. This alteration could affect the genetic structure of wild switchgrass populations, which are native to the eastern half of North America through cultivar introgression. In this study, PCR amplification of microsatellite fragments as well as chloroplast gene-specific markers were utilized to quantify the genetic diversity and structure of five native populations and three agronomic fields (hereafter ‘populations’) planted with switchgrass cultivars. Microsatellite polymorphism across all the switchgrass populations ranged from 91.4 to 100 %. Overall, natural switchgrass populations had significantly higher mean genetic diversity than agronomic switchgrass cultivars (0.262 ± 0.102 and 0.201 ± 0.082 respectively, t test p < 0.008). Natural switchgrass populations had significantly higher total genetic diversity within (HS) and among (HT) as compared to agronomic switchgrass cultivars. A clear separation of natural and agronomic switchgrass populations was noted using principal component analysis and STRUCTURE analysis. A grouping pattern similar to that obtained in the microsatellite study was observed when chloroplast nucleotide sequence variation was assessed. In the realm of bioenergy sustainability, our results highlight the need to consider the genetic structure of cultivars for bioenergy when they are grown in proximity to native switchgrass populations. 相似文献
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Genetic diversity and structure of natural and agronomic switchgrass (Panicum virgatum L.) populations 总被引:1,自引:1,他引:0
Madhugiri Nageswara-Rao C. Neal Stewart Jr. Charles Kwit 《Genetic Resources and Crop Evolution》2013,60(3):1057-1068
Panicum virgatum L. (switchgrass) is an obligate outcrossing C4 perennial prairie grass currently being pursued for the production of lignocellulosic ethanol. Commercial production of switchgrass for bioenergy has increased substantially in the United States. Understanding the degree of native genetic diversity within and among switchgrass populations will facilitate effective germplasm improvement, conservation, and management programs. In this study, the genetic diversity and differentiation among natural and agronomic switchgrass populations were analyzed at the molecular level by using random amplified polymorphic (RAPD) DNA markers. The mean genetic diversity among populations ranged from 0.051 ± 0.136 to 0.243 ± 0.214 and the mean genetic similarity among all the switchgrass populations was 0.775. The clustering pattern of switchgrass populations grouped the individuals based on their sites of origin, with agronomic cultivars predominantly separated into distinct clusters. The grouping of individuals within and across the populations was corroborated by principal component analysis. These results are consistent with previous reports for switchgrass accessions. RAPD DNA markers were suitable for quickly estimating the genetic diversity of native and agronomic switchgrass populations, and suggest that introgression of agronomic genes into natural switchgrass populations and subsequent changes in genetic structure may be detectable. 相似文献
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