Growth and development of Cuscuta chinensis Lam. and its impact on selected crops

The growth and development of Cuscuta chinensis Lam., and the effects of the holoparasite on the growth and yield of tomato ( Lycopersicon esculentum ), chilli ( Capsicum annuum ) and rice ( Oryza sativa ) were investigated in pot experiments under glasshouse conditions. 'Mature' and 'immature' seeds of C. chinensis showed 15 and 47% germination, respectively. The independent life duration of seedlings from 'immature' seeds was 6 days shorter than 'mature' seeds (19 days). Cuscuta chinensis formed haustoria on chilli and tomato, but not on rice seedlings. Infection with three C. chinensis seedlings per host plant reduced leaf area, dry weight, carbon and nitrogen content by 78–84% and 27–44% in tomato and chilli plants, respectively. Dry weight of C. chinensis was 0.63 g per pot in tomato, and 0.24 g per pot in chilli. Carbon and nitrogen content of C. chinensis was 0.189 and 0.028 g per pot, respectively, when grown with tomato. When it was grown with chilli, the carbon and nitrogen content of C. chinensis was 0.001 and 0.007 g per pot, respectively. Cuscuta chinensis reduced the fruit yield of tomato and chilli by 72 and 29%, respectively. The results indicated that tomato is a preferred host of C. chinensis compared with chilli, and rice is not preferred. In the host–parasite associations, C. chinensis suppressed growth and yield of chilli and tomato by diverting the elaborated products of metabolism of the hosts.     

Impact of crop rotation and minimum tillage on water use efficiency of irrigated cotton in a Vertisol

Crop water use efficiency of irrigated cotton was hypothesized to be improved by a combination of minimum tillage and sowing a wheat (Triticum aestivum L.) rotation crop. This hypothesis was evaluated in a Vertisol near Narrabri, Australia from 1997 to 2003. The experimental treatments were: continuous cotton sown after conventional or minimum tillage and minimum-tilled cotton–wheat. Soil water content was measured with a neutron moisture meter, and runoff with trapezoidal flumes. Application efficiency of irrigation water was estimated as the amount of infiltrated water/total amount applied. Plant available water was estimated using the maximum and minimum soil water storage during the growing season. Evapotranspiration was estimated with the water balance method using measured and simulated soil water data. Seasonal evapotranspiration was partitioned into that coming from rainfall, irrigation and stored soil water. Crop water use efficiency was calculated as cotton lint yield per hectare/seasonal evapotranspiration. Rotation of cotton with wheat and minimum tillage improved water use efficiency in some years and application efficiency in all years. Average seasonal evapotranspiration was higher with minimum tillage than with conventional tillage. In years when cotton was sown in all plots, average cotton crop water use efficiencies were 0.23, 0.23 and 0.22 kg (lint)/m³ for minimum-tilled cotton–wheat and continuous cotton, and conventionally tilled continuous cotton, respectively. In-season rainfall efficiency, transpiration and soil evaporation were unaffected by cropping system.     

Alteration in the apoptosis process of rat esophageal epithelium with hyperproliferation of indigenous bacteria under a physiological condition

The apoptosis process in rat esophageal epithelium was investigated using enzyme-immunohistochemistry and transmission electron microscopy. As a result, Fas and Fas-L were expressed in the epithelial cell membrane and cytoplasm from the stratum spinosum (SS) to the stratum granulosum (SG). No TNF-R1 show immunopositivity in the cell membranes. TNF-α and caspase-8 were not observed in any layer. Caspase-10, cleaved caspase-3, XIAP and DNase-1 were found in the epithelial cytoplasm from the SS to the SG, whereas Bid, Apaf-1 and cleaved caspase-9 were detected only in the SG. Cytochrome c was observed as cytoplasmic granular positivity from the stratum basale (SB) and altered into homogeneous immunopositivity in the SG. Bcl-2 and Bcl-X immunopositivity was detected in cytoplasm from the SB to the SG. Immunoreactions of Bak in the cytoplasm and Bax beneath the cell membrane were observed from the upper portion of the SS with increasing intensity toward the SG. In the sites with the hyperproliferation of indigenous bacteria, TNF-R1, TNF-α and caspase-8 were detected in the SG and the immunopositive intensities of Bid, Apaf-1 and cleaved caspase-9 were altered to be strong. Prominently swollen cells and decreased mitochondria were ultrastructurally confirmed in the uppermost layers of stratum corneum. These findings suggest that the Fas-Fas-L-interaction initially induces apoptosis through a mitochondria-independent pathway and secondarily through a mitochondria-dependent pathway, leading to eventual epithelial cell death in the rat esophageal epithelium. The bacterial stimuli probably enhance the mitochondria-dependent pathway through the TNF-R1-TNF-α interaction.     

Comparative Study on Growth Performance of Transgenic(Over-Expressed OsNHX1) and Wild-Type Nipponbare under Different Salinity Regimes

Transgenic Nipponbare which over-expressed a Na+/H+ antiporter gene OsNHX1 was used to compare its growth performance, water status and photosynthetic efficiency with its wild type under varying salinity regimes. Chlorophyll content, quantum yield and photosynthetic rate were measured to assess the impact of salinity stress on photosynthetic efficiency for transgenic and wild-type Nipponbare. Effects of salinity on water status and gas exchange to both lines were studied by measuring water use efficiency, instantaneous transpiration rate and stomatal conductance. Dry shoot weight and leaf area were determined after three months of growth to assess the impacts of salinity on the growth of those two lines. Our study showed that both lines were affected by salinity stress, however, the transgenic line showed higher photosynthetic efficiency, better utilization of water, and better growth due to low transpiration rate and stomatal conductance. Reduction of photosynthetic efficiency exhibited by the wild-type Nipponbare was correlated to its poor growth under salinity stress.     

Immunohistochemical detection of phosphatidylserine and thrombospondin on denucleating erythroblasts in rat bone marrow

The hypothesis that apoptotic factors play some roles in the denucleation of erythroblasts has been confirmed by the immunohistological detection of both phosphatidylserine and thrombospondin as phagocytosis-inducing factors in general apoptotic events. Both phosphatidylserine and thrombospondin were detected on the surface of cell membrane of mature erythroblasts, while thrombospondin was also detected in more immature erythroblasts. The intensities of their immune reactions increased as the erythroids matured. During denucleation, the positivities of both phosphatidylserine and thrombospondin were restricted on the surface of the cell membrane surrounding the protruding nuclei. Thus, the apoptotic process involves denucleation of erythroblasts and phosphatidylserine, and thrombospondin acts as phagocytosis-inducing factors in the denucleation event.     

Photosynthesis in co-occurring invasive Acacia spp. and native Bornean heath forest trees at the post-establishment invasion stage

Seedlings of invasive species often exhibit superior physiological traits that facilitate their spread at early stages of invasion, although it is unclear whether these traits persist at the post-establishment stage. To determine whether mature exotic Acacia spp. possess superior traits over mature native plants, we compared foliar gas exchange and chlorophyll a fluorescence of Acacia auriculiformis and Acacia mangium coexisting with tropical heath forest tree species Buchanania arborescens and Dillenia suffruticosa in Brunei Darussalam. The CO₂ assimilation rates of Acacia spp. were significantly higher than those of heath species at current prevailing conditions of ~400 ppm ambient CO₂, 1,500 µmol m^?2 s^?1 photosynthetically active radiation and 30°C leaf temperature. The photosystem II of Acacia spp. exhibited significantly higher maximum quantum yield of primary photochemistry at comparable temperatures, and was more sensitive to an elevated temperature (42°C for 1 h). Better photosynthetic performance of Acacia spp., due to larger stomatal openings, better light harvesting efficiency, and greater plasticity in photosystem II, may enable adult Acacia trees to sustain a competitive growth advantage and suppress native tropical heath forest species. The competitive advantage maintained by Acacia spp. in post-establishment stage likely facilitates the establishment of monospecific Acacia stands in invaded heath forests.     

Immunohistochemical and histoplanimetrical study on the spatial relationship between the settlement of indigenous bacteria and the secretion of bactericidal peptides in rat alimentary tract

To clarify the regulatory mechanism by bactericidal peptides secretion, the secretion of bactericidal peptides was immunohistochemically and histoplanimetrically compared with the degree of Gram-positive/negative bacterial colonization throughout the rat alimentary tract. In the associated exocrine glands from the oral cavity to the stomach, no comparable differences were observed under the changes of development of indigenous bacterial colonies. In the small intestine, immunopositive granules for lysozyme and secretory phospholipase A2 (sPLA2) were markedly decreased, whereas immunopositive vacuoles in the Paneth cells were more increased at sites with hyper-development of indigenous bacterial colonies in the intervillous spaces than at sites with no or less development. No changes in exocrine glands were observed in the large intestine because of the constant existence of large quantities of bacteria. Gram-positive bacterial colonies on the mucosal surfaces were dominant from the oral cavity to the stomach. Gram-negative bacteria were dominant in the large intestine, and the distributions of both Gram-positive and negative bacteria were intermediate in the small intestine. These findings suggest that lysozyme and sPLA2 secreted from the Paneth cells contribute to the regulation of the proliferation of indigenous bacteria in the intervillous spaces of the small intestine, and that the inversion of distributions of Gram-positive and -negative bacteria in the alimentary tract might be caused by the secretion of lysozyme and sPLA2 in the small intestine.     

Botryosphaeriaceae species associated with blueberry dieback and sources of primary inoculum in propagation nurseries in New Zealand

Sampling at blueberry farms found Botryosphaeriaceae fungi in five of seven farms sampled; overall incidence was 41.4%, with Neofusicoccum australe (79.0%), N. luteum (8.0%), N. ribis (8.0%) and N. parvum (5.0%). Sampling of nursery plants found infections in all four nurseries with 45% incidence in mainly asymptomatic plants, which were infected with N. australe (66.0%), N. parvum (31.5%) and N. ribis (2.5%). Asymptomatic propagation cuttings from one nursery were found to have external contamination of Botryosphaeriaceae DNA (90.0%) and internal infection (65.0%) by the four main species found in the blueberry farms and nurseries. For propagation media nested PCR showed that out of the 98 samples received, 43 samples were positive for the presence of Botryosphaeriaceae DNA (44.0%). Results from the SSCP indicated that N. australe, N. luteum, N. parvum/ N. ribis and Diplodia mutila were present in the propagation media received. Isolates of the four main species recovered from farms and nurseries were pathogenic on blueberry stems but pathogenicity differed significantly between species and isolates within a species, with N. ribis being the most pathogenic, then N. parvum, N. luteum and N. australe. Overall, the high rate of infection in nursery plants indicated that nurseries can be a major source of infection for blueberry farms. Since propagating cuttings are the likely sources of infection for nurseries, these should be targeted in the control strategies.     

Impact of Cuscuta australis infection on the photosynthesis of the invasive host,Mikania micrantha,under drought condition

Cuscuta species (dodders) are widespread stem holoparasites that depend on host plants for their entire mineral and water and most carbohydrate requirements. Dodders negatively affect host photosynthesis but precise information on their impact on hosts in the presence of environmental stress factors (i.e. drought) is little known. In a pot experiment, the leaf traits, gas exchange and chlorophyll a fluorescence of the invasive climber, Mikania micrantha, parasitized by Cuscuta australis, were investigated in order to study variations of host photosynthesis in response to parasitism and drought. The results showed that the concomitant presence of C. australis infection and drought significantly impacted the leaf traits (i.e. increased leaf dry mass content), gas exchange (i.e. decreased stomatal conductance and transpiration rates and increased water‐use efficiency) and quantum yield of chlorophyll a fluorescence of M. micrantha. The presence of a single stress factor (C. australis infection or drought), however, only significantly affected the leaf traits and gas exchange of M. micrantha. These results suggested that the combined additive effects of C. australis parasitism and drought significantly suppressed the photosynthesis of M. micrantha in relation to both stomatal and non‐stomatal limitation of host photosynthesis. This study provides insights into Cuscuta–host interactions under drought conditions in the tropics.