萘乙酸对大白菜钙素吸收运转及防治干烧心病的研究

本研究从补钙入手,探索防治干烧心病的方法。用0.7％CaCl_2·2H_2O对和50ppmNAA混合液在大白菜结球期叶面喷洒5次,经8年试验,对大白菜干烧心病的相对防治效果达80％以上。 在此基础上,采用~(45)Ca示踪试验的方法对防病机理做了进一步研究。结果表明,无论从根部还是从叶部加萘乙酸对根部和叶部~(45)Ca的吸收和运转均具有促进作用,并且改变了其在大白菜植株内的分配规律。对照株单位干叶重dpm值自外向内呈下降趋势(130×10~3─60×10~3);用NAA处理株则相反,自外向内呈增加趋势(120×10~3─250×10~3),说明萘乙酸具有促进钙素向叶球中内部运转的功效。这种效果在贮藏期间仍能保持。另外,~(45)Ca向直接受到NAA处理部位的运转量有明显增高的趋势。这一结果为补钙防治干烧心病提供了理论依据。     

苹果果皮细胞膜结构变化与虎皮病的关系

国光苹果在贮藏过程中,果皮内α—法尼烯不断积累,60天左右达最大值,后随共轭三烯的迅速增加而下降。与此同时,果皮中丙二醛积累,组织相对电导率增加。抗氧化剂二苯胺能显著降低果皮中丙二醛积累,阻止相对电导率的上升,减少发病率。对膜结构变化与虎皮病的关系进行了讨论。     

以CP23重组蛋白为抗原建立检测微小隐孢子虫抗体间接ELISA方法的研究

摘要：以在E.coli高效表达的微小隐孢子虫子孢子表面抗原CP23为抗原，以辣根过氧化物酶(HRP)标记的山羊抗鼠IgG为二抗，建立了检测微小隐孢子虫抗体的间接ELISA方法。经检测筛选出最佳反应条件为1μg／孔纯化的E.coli表达的CP23抗原包被酶标板，用10％免血清进行封闭，以正常E.coli裂解上清液稀释待检血清。实验表明应用CP23重组蛋白作为诊断C.parvum抗原具有特异性高、抗原易纯化和成本低等特点。     

胶合板厂生产规划模型与运用

本文根据胶合板厂实际情况，建立了杨木胶合板厂生产规划模型。该模型考虑各主要设备工段、产品组合、产品混合、产品价格、流动资金、原材料供应以及市场约束等问题。本模型能够满足市场竞争，获取最佳利润，同时还能解决胶合板厂“三板”（表、背、芯单板）不平衡问题。     

Comparison of morphology, viability, and function between blood and milk neutrophils from peak lactating goats

The morphological features of blood and milk neutrophils from peak lactating goats were compared using light microscopy, scanning electron microscopy and flow cytometry in order to investigate the cytological changes of neutrophils after migration into the mammary gland. The kinetics of reactive oxygen intermediates (ROI) generation and gelatinase release of blood and milk neutrophils, with or without stimulation of phorbol 12-myristate, 13-acetate ester (PMA), were used to characterize their responses to inflammatory stimuli. Neutrophils isolated from goat milk were highly segmented and contained multi-lobed nuclei. Ultrastructurally, milk neutrophils were more ruffled on the surface compared to blood neutrophils. Approximately 30% of milk neutrophils were undergoing cell death, either necrosis or apoptosis, in contrast to 8% of blood neutrophils. The ROI production of activated milk neutrophils peaked earlier than blood neutrophils, but the duration and the intensity were much less. Neutrophils from both sources augmented the release of gelatinase in response to PMA (1 ng/mL). However, the amount of gelatinase released from milk neutrophils was lower (P < 0.05) than that of blood neutrophils. In summary, more neutrophils become apoptotic and necrotic in the mammary gland, presumably due to spontaneous aging, the process of diapedesis, and the interaction with milk components. Milk neutrophils have impaired functionalities in comparison with blood neutrophils. The information is relevant when studying mammary gland immunity and related diseases, such as mastitis.     

Effects of Confluent, Roscovitine Treatment and Serum Starvation on the Cell-cycle Synchronization of Bovine Foetal Fibroblasts

The present study was designed to examine the effects of cell-cycle synchronization protocols, such as confluent, roscovitine treatment and serum starvation, in bovine foetal fibroblasts on synchronization accuracy at G0/G1, viability, apoptosis, necrosis and ploidy for use as a nuclei donor. The cells in 5-10 passages were randomly allocated into three treated groups. Cells were cultured either in Dulbecco's modified Eagle's medium (DMEM) + 10% foetal bovine serum (FBS) until 90% confluent (group 1, confluent), in DMEM + 10% FBS + 30 microM roscovitine for 12 h (group 2, roscovitine), or in DMEM + 0.5% FBS for 5 days (group 3, serum starvation). Most of the cells (>80%) in all groups were arrested at the G0/G1 stage. Although the rates did not differ, cells in group 1 showed an increased cell population arrested at the G0/G1 phase. Significantly (p < 0.05) higher rates of apoptosis occurred in group 3 than in group 1 and 2 (10% vs 6% and 6%, respectively). No differences in chromosomal abnormality were observed among groups. However, by increasing the number of cell culture passages up to 15, significantly (p < 0.05) higher chromosomal abnormality was observed than in 5 and 10 passages (39% vs 28% and 23%, respectively) in group 1. The results clearly indicated that bovine foetal fibroblasts could be effectively synchronized at G0/G1 stages by all the three different treatments, confluent, roscovitine and serum starvation. However, cells in confluent showed reduced apoptosis and necrosis when they underwent 5-10 passages, exhibiting increased percentage of cells with stable chromosome diversity. Hence, cells in confluent merit further studies before they could be used as nuclear donors.     

‘三棱榄''橄榄果实香气成分分析

1 材料与方法选取广东优良鲜食橄榄品种‘三棱榄’,2001年12月6日采样,采用固相微萃取法(SPME)富集香气成分(鲜橄榄果肉于15℃下捣碎后取样1.0 g放入4 mL聚四氟乙烯硅橡胶垫密封螺口玻璃瓶中,插入100μm聚二甲基硅氧烷纤维头于室温25-30℃顶空取样2 h),用美国Finnigan TRACE GC-MS气相色谱-质谱联用仪进行分析。气相色谱柱为DB-1弹性毛细管柱30 m×0.25 mm,载气为He(99.99％),流速1.0mL/min。程序升温从40℃开始先保持10 min,后以2℃/min的升温速率升至150℃保持10 min。质谱条件:电子能量70 eV,离子源温度250℃,质量范围35-450 aum,不分流进样。2002年12月18日采样重复分析。     

2018年安徽省部分地区A型禽流感流行率横断面研究

为了解安徽省A型禽流感病毒感染情况,2018年4—5月,在安徽省北部、中部和南部7个市,随机选取各类型场点173个,在每个场点内随机采集35份咽喉/泄殖腔和环境拭子样品,使用荧光RT-PCR进行A型、H5亚型、H7亚型流感病毒核酸检测。结果显示：在4个养殖场、3个活禽批发市场和17个农贸市场检出了A型禽流感病毒,场点阳性率分别为2.9%（4/140）、75.0%（3/4）、80.9%（17/21）,未检出H5和H7亚型流感病毒核酸;A型禽流感群体流行率为11.2%（95%CI：6.5%~15.8%）,个体流行率为1.2%（95%CI：0.9%~1.5%）;活禽交易市场内,禽群感染A型禽流感病毒的风险较高（OR=136,95%CI：32.7~549.4）。结果表明,A型禽流感病毒在养殖和交易环节存在一定的污染,而在活禽交易市场的污染更严重,是今后禽流感防控的重点,需继续加强活禽交易市场的管理和消毒灭源工作。     

Effects of Dietary Supplementation of Barodon on Growth Performance,Innate Immunity and Disease Resistance of Juvenile Olive Flounder,Paralichthys olivaceus,Against Streptococcus iniae

This study was conducted to evaluate the effects of dietary supplementation of Barodon, an anionic alkali mineral complex, on growth, feed utilization, humoral innate immunity and disease resistance of olive flounder. A basal experimental diet was used as a control and supplemented with 0.1, 0.2, 0.3, 0.4, or 0.5% Barodon. Triplicate groups of fish (26.4 ± 0.2 g) were fed one of the diets to apparent satiation twice daily for 10 wk. The growth performance was enhanced (P < 0.05) linearly and quadratically in fish fed diets containing Barodon compared with that in fish fed the control. Feed utilization was significantly improved by Barodon supplementation. Serum lysozyme and antiprotease activities were increased quadratically in Barodon fed groups. Also, significantly higher superoxide dismutase activity was found in Barodon‐fed fish. Dietary supplementation of 0.1–0.3% Barodon resulted in significant enhancement of fish disease resistance against Streptococcus iniae. The findings in this study indicate that dietary supplementation of Barodon can enhance growth, feed utilization, innate immunity, and disease resistance of olive flounder and that the optimum level seems to be 0.1% in diets.