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Unfermented C. genistoides methanol extracts of different harvestings and selected polyphenols were evaluated for phytoestrogenic activity by comparing binding to both ER subtypes, transactivation of an ERE-containing promoter reporter, proliferation of MCF-7-BUS and MDA-MB-231 breast cancer cells, and binding to SHBG. The extracts from one harvesting of C. genistoides (P104) bound to both ER subtypes. All extracts transactivated ERE-containing promoter reporters via ERbeta but not via ERalpha. All extracts, except P122, caused proliferation of the estrogen-sensitive MCF-7-BUS cells. Proliferation of MCF-7-BUS cells was ER-dependent as ICI 182,780 reversed proliferation. Physiologically more relevant, extracts antagonized E2-induced MCF-7-BUS cell proliferation. Furthermore, all extracts, except P122, induced proliferation of the estrogen-insensitive MDA-MB-231 cells, suggesting that the extracts are able to induce ER-dependent and ER-independent cell proliferation. Binding to SHBG by extracts was also demonstrated. These results clearly show that C. genistoides methanol extracts display phytoestrogenic activity and act predominantly via ERbeta. HPLC and LC-MS analysis, however, suggests that the observed phytoestrogenic activity cannot be ascribed to polyphenols known to be present in other Cyclopia species. 相似文献
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Andrew Kiggundu Clifford S. Gold Maryke T. Labuschagne Dirk Vuylsteke Schalk Louw 《Euphytica》2003,133(3):267-277
Forty-five Musa clones, including endemic and introduced cultivars plus hybrids, were evaluated for resistance against the banana weevil,
Cosmopolites sordidus, in a field trial in Uganda. The predominant groups of staple crops, East African highland bananas (Musa spp. AAA) and plantains (Musaspp. AAB), as well as plantain-derived hybrids (AAB × AA), showed the highest levels of susceptibility to this pest. These
were followed by dessert bananas (Musa spp. AAA), exotic bananas (Musa spp. ABB) and finally diploids of M. acuminata (AA). Hybrids of banana origin were highly resistant. Some East African highland cultivars, especially brewing types (e.g.,
Kabula, Bagandeseza, Ediirira), showed intermediate levels of resistance. Among the non-highland bananas, high levels of resistance
were observed in Yangambi-Km5 (AAA), Cavendish (AAA), Gros Michel (AAA), Kayinja (ABB, Pisang Awak subgroup), Ndiizi (AB,
Ney Poovan subgroup)and Kisubi (Ney Poovan subgroup). The highest resistance was observed in banana hybrids TMB2×7197-2, TMB2×8075-7
and the wild banana Calcutta-4 (AA). These were considered the best sources of resistance for a weevil resistance-breeding
programme with the two hybrids commonly used as improved male parents.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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Armin H.W. Seydack Graham DurrheimJosua H. Louw 《Forest Ecology and Management》2011,261(7):1152-1169
Stand-level tree diameter growth patterns were explored for evergreen moist forests in the southern Cape, South Africa. Results of standard multiple regression analyses, involving 934 permanent sample plots with data spanning a 10-year interval, revealed that stand-level increment of canopy species in the canopy layer (>30 cm dbh) was significantly determined by inherent species-specific growth capacities (species composition of the stand), water availability, forest matrix crowding and tree condition impairment (age-related manifestations of reduced vitality indicated by signs of crown die-back, damage and stem rot). In contrast, stand-level increment of trees of canopy species in the subcanopy layer (10-20 cm dbh) was prominently shaped by light availability, as mainly determined by the degree of canopy-level disturbance (mortality rate of trees >30 cm dbh), crowding (canopy-level overhead and forest matrix crowding) and proximity to conspecific adults (within 6-8 m). In addition to species-inherent and resource factors, considerable variation in stand-level growth resulted from site-climate interactions. For 507 of the permanent sample plots, increment data was available for two consecutive 10-year intervals; permitting the analysis of spatiotemporal interactions of growth patterns (repeated measures ANOVA). In the Knysna forests higher canopy-level increment rates were associated with the moister southerly facing slope sites in comparison with the drier northerly facing and ridge sites during the first increment period. During the second increment period, increment rates on the drier, but better illuminated sites had increased disproportionately. In contrast, in the Tsitsikamma forests, higher increment rates during the second increment period were encountered on moister flat bottomland sites (with extended periods of subsoil wetness) than on the comparatively drier southerly facing slope sites (increment period × site-based water availability × forests interaction). In both forests relatively higher growth performance of subcanopy-level trees during the second increment period was associated with stands experiencing conditions of enhanced light availability. Atmospheric temperatures were higher during the second increment period (mean periodic Tmax: + 0.64 °C). The detected spatiotemporal interactions were interpreted as site × climate interactions where site-related conditions of favourable light or water availability resulted in enhanced temperature-linked growth responses during the second increment period. A metabolic performance trade-off model provided a framework for the interpretation of these complex site-climate interactions by placing the patterns of forest growth into an ecophysiological explanatory context. 相似文献
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Summary Isozyme analyses have been used for the definitive identification of many plant cultivars, but not for cultivated tomatoes. Six isozyme systems, namely alcohol dehydrogenase, acid phosphatase, phosphoglucomutase, esterase, phosphoglucoisomerase and 6-phosphogluconate dehydrogenase of tomato seed extracts were resolved by isoelectric focusing on polyacrylamide gels with a narrow pH gradient. Nine alcohol dehydrogenase phenotypes were distinguished which, with three acid phosphatase phenotypes, identified twelve of the seventeen cultivars. Fewer differences were found for the other isozymes. Since this method could differentiate between breeding parents and their progeny it is concluded that further investigations are warranted.Abbreviations APS
acid phosphatase
- ADH
alcohol dehydrogenase
- EST
esterase
- IEF
isoelectric focusing
- PGI
phosphoglucoisomerase
- PGM
phosphoglucomutase
- 6-PGDH
6-phosphogluconate dehydrogenase
- RFLP
restriction fragment length polymorphism
- VOPRI
Vegetable and Ornamental Plant Research Institute 相似文献
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The seasonal incidence of helminth infestation in sheep on newly established irrigated pastures was determined by the slaughter of pairs of tracer lambs exposed for periods of approximately 33 days from October 1968--July 1970 and of sets of 3 lambs from March 1971--May 1973. It took several months for the infestation to become established on the pasture, but thereafter, of the various species present; Haemonchus contortus was most prevalent. In general, peak burdens of this species were recovered from January--May or June, while marked inhibition of larval development was evident from April--August. Ostertagia circumcinta usually occurred in increased numbers from April--October, and larval development was inhibited during July and August. Although the sizes of the burdens varied considerably from year to year, Trichostrongylus spp. were present mainly from April--August. Oesophagostomum columbianum, although present in the lambs slaughtered during the first 5 months of the survey, virtually disappeared until May 1971. After this it reappeared in small numbers in nearly all the sheep, reaching peak burdens during April and May 1972, and May 1973. Moniezia expansa were generally recovered from the lambs slaughtered from November--May. 相似文献
10.
Bryson NR Horak IG Höhn EW Louw JP 《Journal of the South African Veterinary Association》2000,71(3):175-179
A total of 344 dogs belonging to people in resource-poor communities in North West Province, South Africa, was examined for ectoparasites, and all visible arthropods were collected from the left side of each dog. By doubling these numbers it was estimated that the dogs harboured 14,724 ixodid ticks, belonging to 6 species, 1,028 fleas, belonging to 2 species, and 26 lice. Haemaphysalis leachi accounted for 420 and Rhipicephalus sanguineus for 14,226 of the ticks. Pure infestations of H. leachi were present on 14 dogs and of R. sanguineus on 172 dogs. Small numbers of Amblyomma hebraeum, R. appendiciulatus, R. evertsi evertsi and R. simus were also collected. The predominance of R. sanguitneus accounts for the high prevalence of canine ehrlichiosis (Ehrlichia canis) within the survey region, compared to canine babesiosis (Babesia canis), which is transmitted by H. leachi, and is a much rarer disease. 相似文献