配料混合试验的设计和分析

系统地介绍了配料混合试验的基本概念．指出了混合试验与常规因子试验的区别。提出配料混合试验的3种设计，即顶点和边界点设计、矩心设计和极顶设计。以2个实例演示了混合试验资料的统计方法，包括回归和方差分析．驻点分析和等值线分析。     

春小麦新品种互麦14号栽培技术

介绍了我省育成的春小麦新品种—互麦14号的丰产栽培技术。     

不确定度评定方法在汽车事故再现中的应用

介绍了不确定度理论及其评定方法，结合车辆碰撞事故的具体特点，给出了基于能量守恒的车速计算表达式．通过选择适当的输入参数作为不确定因子，分别计算各分量等效速度标准不确定度．考虑不确定度累积和传播，得到车速输出结果的合成标准不确定度和展伸不确定度，以此检验事故再现结果的准确程度．通过具体事故案例的分析计算和计算机检验，验证该方法的适用性．     

犬埃立克体实验模型初步研究

将犬埃立克体标准毒株（Florida株）体外细胞纯培养物和我国犬埃立克体病病犬血白细胞分别接种于C3H小鼠，接毒后d，经PCR检测证实均感染成功。本试验为犬埃立克体病小动物模型的建立打下了基础。     

RT-PCR及RFLP分析技术对鸡传染性支气管炎病毒的诊断和S1基因分型的研究

本研究使用分别扩增整个S1糖蛋白基因 (引物A)和S1糖蛋白基因N_端高变区Ⅰ (引物B)的 2对引物对 3个IBV标准株M41、Connecticut、Arkansas及 5个地方分离株 (C60 ,D41A ,D41B ,A112 1,A1171)进行RT_PCR扩增。用引物A时 ,有 5个IBV毒株扩增到目的片段 (172 0bp) ;用引物B时 ,所有 8个IBV毒株均得到与预计大小相符的目的产物 (2 2 8bp)。对 172 0bp的PCR产物用限制性内切酶HaeⅢ进行酶切 ,结果得出 3个不同的RFLP图谱 ,其中M41、Connecticut、D41B具有相同的HaeⅢ酶切图谱。Arkansas和D41A则分别具有互不相同的图谱 ;对 2 2 8bp的PCR产物进行DdeⅠ、RsaⅠ限制性内切酶消化 ,根据它们的RFLP图谱 ,8个IBV毒株可分为 5个基因型。综合 2对引物的PCR产物的RFLP分析结果 ,8个IBV毒株可分为 7个基因型 ,分型的结果与传统的血清学方法吻合。本研究建立的方法和技术具有快速、简单、特异、灵敏等优点 ,为现场流行毒株的定型(基因型 /血清型 )及其S1基因变异的跟踪研究以及更有效防制传染性支气管炎奠定了基础。     

Thrombocytopenia in Cats: A Retrospective Study of 41 Cases

The prevalence of feline thrombocytopenia (<200,000 platelets/L) at North Carolina State University, College of Veterinary Medicine Teaching Hospital, from January 1985 to March 1990, was 1.2% (41/3300). Cats were divided into six categories based on clinical diagnoses: 29% (12/41) had infectious disease, 20% (8/41) had neoplasia, 7% (3/41) had cardiac disease, 2% (1/41) had primary immune-mediated disease, 22% (9/41) had multiple diseases, and 20% (8/41) had disorders of unknown etiology. The mean platelet count for all thrombocytopenic cats was 52,000/μL ± 46,000/μL (1 SD) with a range of 1000–190,000/μL. No significant differences were found between groups with respect to platelet count, packed cell volume, or white blood cell count, though anemia and leukopenia were common among the cats as a whole. Bleeding disorders (hemorrhage or thrombosis) were observed in 29% (12/41) of thrombocytopenic cats and were more likely to be associated with neoplasia, cardiac disease, and platelet counts less than or equal to 30,000/μL. Disseminated intravascular coagulopathy was diagnosed in 12% (5/41) of the cats. Infections and/or neoplasia affecting the bone marrow were the most common diseases associated with thrombocytopenia. Feline leukemia virus and myeloproliferative neoplasia accounted for approximately 44% (18/41) of the specific diagnoses in thrombocytopenic cats. (Journal of Veterinary Internal Medicine 1993; 7:261–265. Copyright © 1993 by the American College of Veterinary Internal Medicine.)     

Book reviews

Recent Advances in Turkey Science. Edited by C. Nixey and T. C. Grey, Poultry Science Symposium Number 21. London, Butterworths, ISBN 0 408 00971 3

Egg and Eggshell Quality. Sally E. Solomon, 1991, 149 pp., illustrated. London, Wolfe Publishing Ltd., £35.00, ISBN 0 7234 1647 8.

Aleen Cust, Veterinary Surgeon, Britain's First Woman Vet. Connie M. Ford, 1990, 109 pp., £5.99, Bristol, Biopress Ltd., The Orchard, Clanage Road, ISBN 0 948737 11 5.

Avian Incubation. Edited by S. G. Tullett, 1991, xiv + 335 pp., illustrated. London, Butterworth‐Heinemann. £00.00, $00.00. ISBN 0–7506–1002–6.

A Colour Atlas of Diseases & Disorders of the Domestic Fowl & Turkey. Edited by C. J. Randall, second edition, 1991, 175 pages, 432 illustrations in colour. London, Wolfe Publishing Ltd, £35, ISBN 0723416281.     

红汁乳菇液体深层发酵培养基的筛选

采用均匀设计法优化红汁乳菇液体深层发酵培养基。结果表明，最佳培养基配方是：蔗糖34g]L，废糖蜜13mL／L，麸皮36g／L，玉米粗粉20g／L，KH2PO43g／L，菌丝体最大生物量达18．087g/L。