Evidence for surfactant contributing to the gastric mucosal barrier of the horse

This study was undertaken to determine the hydrophobicity of the luminal surface of the equine stomach and to elucidate the ultrastructure of the lining imparting that property. Gastric and duodenal mucosal samples from 5 horses were collected immediately after euthanasia and subjected to surface contact angle measurement using a goniometer. Gastric mucosal samples from 4 horses and a foal were examined by electron microscopy following a fixation procedure known to preserve phospholipids and oligolamellar structures. Contact angles for the equine gastric glandular mucosal surface (mean +/- s.e. 78.0 +/- 11.0 degrees) were greater than for the duodenum (33.4 +/- 8.7 degrees), (P = 0.003). The contact angles for gastric squamous tissue (50.4 +/- 4.5 degrees) tended to be greater than for duodenum (P = 0.15). Electron microscopy revealed the existence of surfactant as abundant osmiophilic phospholipid material within both squamous and glandular gastric mucosae. These results indicate the hydrophobic nature of the equine gastric mucosae. We propose that the water-repellent nature of the stomach contributes to the 'gastric mucosal barrier' and is imparted by surface-active phospholipid adsorbed to the surface. Phospholipids may also be utilised as a physical barrier to back-diffusion of acid by lining intracellular canaliculi and oxyntic ducts where other defence mechanisms are absent.     

Distribution of circulation rates within a single tissue type

An analysis is undertaken to determine the continuous distribution of circulation rates which would be necessary for a single tissue type to display a simple exponential response for the exchange of a solute with blood. It is shown that there can be no more than one real solution. This indicates that heterogeneous circulation cannot be invoked to explain anomalies to the popular assumption that blood perfusion is the process limiting the rate of blood-tissue exchange.     

Telecommunications in alaskan villages

A recently installed satellite system now provides modern long-distance telecommunication services to 100 rural Alaskan villages, most of whose residents are Alaska Natives. In most villages no local telephone or television distribution facilities have yet been installed. Local telephone exchange service appears to be economically marginal unless modest regulatory changes are made. Television delivery presents more difficult problems involving technical and organizational structure. If, after weighing the potential social and cultural effects, village residents elect to acquire television, a delivery system based on low-power transmitters in the villages, local government as the basic organizational and economic unit, and a statewide nonprofit service organization, is the feasible system best suited to village needs.     

Chemical facilitation of thermal conduction in physiological systems

Experimental evidence supports the concept that solutes capable of reversible chemical reaction can passively facilitate thermnal conduction within their solutions. Myoglobin and bicarbonate are suggested as energy carriers in muscle, having the combined capacity for conveying all waste metabolic heat produced under normal physiological conditions. The concept is extended to convective heat transfer in vivo; this implicates hemoglobin.     

Surface-active phospholipid (surfactant) in equine tendon and tendon sheath fluid

AIM: To investigate the presence of surface-active phospholipid (SAPL, or surfactant) in equine tendon and tendon sheath fluid. METHODS: The left front flexor tendon and sheath were removed from five Thoroughbred horses. Phospholipid was extracted from tendon sheath fluid using Folch reagent and quantified using spectroscopy. Transmission electron microscopy (TEM) was used to observe the tendon surfaces. RESULTS: The presence of phospholipid (90.6 (SD 4.3) microg/ml) in tendon sheath fluid, plus the appearance of oligolamellar layers and lamellar bodies on the tendon surface were indicative of SAPL. CONCLUSIONS: Evidence of SAPL was found in equine tendon, and may have a similar lubricating function as reported for synovial joints. CLINICAL RELEVANCE: These findings may have important implications for normal tendon function and possible therapeutic adjuncts for tendon and tendon sheath injuries.     

Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis

ABSTRACT: Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells from Arabidopsis leaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K+ and anion channels to that achieved previously in work with Vicia and tobacco guard cells.     

Nitrogen fertilization value of compost and dried dung in soils with contrasting synthetic nitrogen fertilization histories

If the production of forage for dairy cattle is to become less reliant on synthetic nitrogen (N) fertilizers, there is need to better understand and account for the N contributed by on-farm and imported organic amendments. A 254-day aerobic soil incubation study (typical length of a growing season in many temperate dairying regions) quantified the inorganic (mineral) N supply from a commercial compost and dried bovine dung (i.e., on-farm effluent solids). Amendments were incubated in soils with contrasting synthetic N fertilization histories (i.e., 70–100 vs. 350–400 kg N/ha per year) to evaluate if higher synthetic N fertilization histories would reduce the lag time that often exists between organic amendment application and significant release of inorganic N for plant uptake. This proposition was based on previous research, which showed greater soil inorganic N availability accelerating organic amendment decomposition. Our experiment did find that the release of inorganic N from evaluated organic amendments was greater in soils with higher synthetic N fertilization histories, but that this effect was not apparent until after the first 6-months of this 9-month experiment. Despite this finding, soils with contrasting synthetic N fertilization histories were not found to differ in their initial inorganic N content, nor microbial activity or other physiochemical properties known to affect N mineralization. Our study highlighted the long-term vision needed when transitioning from synthetic N fertilizers to organic amendments, with most of the N present in the compost and dried dung remaining unavailable for forage production (i.e., remained bound in organic carbon-based molecules).