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H. Karimi R. Mansoori Ale Hashem G. Ardalani R. Sadrkhanloo H. Hayatgheibi 《Anatomia, histologia, embryologia》2014,43(6):423-428
The vomeronasal organ (VNO) is a tubular structure in the roof of nasal cavity. The important role of this organ is olfaction of sexual odour. In this study, position, anatomical structure and histology of VNO in Iranian camels (camelus domesticus var. dromedaris persica) were determined. Fourteen healthy male camel heads were collected from an industrial slaughterhouse in Tehran, Iran, for anatomical and histological studies (seven each). The length of VNO and width of dental pad and the number and width of palatine crests were measured. For anatomical studies, the mandible was removed, and maxilla and nasal cavity was cut longitudinally and transversely. For histological studies, the mandible was removed, and first 0.5 cm of initial part of VNO was cut. Then, nasal cavity was cut in some segments with 2 cm thickness. The width of VNO was 3.85 ± 0.31 cm and 1.57 ± 0.18 cm in front and distal parts, respectively. The length of VNO was 15.61 ± 0.59 cm. In histological examinations, VNO was surrounded by J‐shape hyaline cartilage. The lining epithelium of lateral wall of VNO was originated from respiratory epithelium, while it had an olfactory epithelium origin in the medial wall. Lamina propria and tunica submucosa were a cavernous connective tissue with seromucous gland with abundant of serous secretory units. The lumen of VNO opens into nasal cavity. The presence of olfactory epithelium found in our study indicates an important role for VNO in pheromone perception and beginning of sexual behaviour. 相似文献
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Ali Barzegar Heshmat Rahimian Haleh Hashemi Sohi 《Journal of General Plant Pathology》2010,76(2):143-151
The nucleotide sequences for the minor coat protein (CPm) gene and its deduced amino acid sequences for two aphid-transmissible
and two nontransmissible isolates of Citrus tristeza virus (CTV) from symptomless orchard trees of Miyagawa satsuma [Citrus unshiu (Macf.) Marc.] on trifoliate orange [Poncirus trifoliate (L.) Raf.] and declining Washington navel [C. sinensis (L.) Osb.] trees on sour orange (C. aurantium L.) rootstocks were analyzed and compared with those of highly transmissible CTV strains available in GenBank. The isolates
produced severe symptoms on indicator plants and their aphid transmissibility was assayed through acquisition by A. gossypii of CTV and subsequent inoculation feeding on young Mexican lime seedlings. The CPm gene nucleotides and coded amino acid
sequences were very similar among the nontransmissible isolates and among the transmissible. Five of 73 nucleotide substitutions
that existed between CPm gene nucleotide sequence of nontransmissible and transmissible isolates caused changes in the deduced
amino acid sequences of the nontransmissible isolates. Two nucleotide substitutions yielded new amino acids with similar properties.
However, the three remaining mutations led to substitution of new amino acids with a different charge and polarity at positions
14, 238 and 239. The last two mutations occurred at the C-terminal region of the CPm, which is implicated in the formation
of a salt bridge that helps to maintain the protein’s tertiary structure. Amino acid substitutions can affect aphid transmission
efficiency by altering the conformation of the proteins or masking motifs involved in the interaction between CPm and aphid
stylets. 相似文献
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Ali Barzegar Haleh Hashemi Sohi Heshmatollah Rahimian 《Journal of General Plant Pathology》2006,72(1):46-51
The biological and molecular properties of four Citrus tristeza virus (CTV) isolates isolated from infected Satsuma trees imported from Japan, and growing in citrus groves in northern Iran (Mahdasht
orchards, Mazandaran Province), were investigated. CTV-infected samples were collected from sweet orange trees and grafted
onto Alemow (Citrus macrophylla Wester) seedlings. On indicator plants, these isolates produced various symptoms including vein clearing and stem pitting
on Mexican lime, Alemow, and Citrus hystrix, and yellowing and stunting on sour orange and grapefruit seedlings. Citrus samples were also surveyed for CTV using serological
tests. The coat protein (CP) gene of these isolates was amplified using specific primers, yielding an amplicon of 672 bp for
all isolates. Sequence analysis showed 98%–99% sequence homology of Iranian isolates with the Californian CTV severe stem-pitting
isolate SY568 and 97%–98% homology with the Japanese seedling yellows isolate NUagA. The Iranian isolates were compared by
restriction fragment length polymorphism (RFLP) analysis of the CP amplicon for further classification. 相似文献
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Saideh Tavallaie Marjan Emtyazjoo Khosrow Rostami Haleh Kosari 《Journal Of Aquatic Food Product Technology》2013,22(1):79-90
A novel Dunaliella salina (D. salina) was isolated from Hoze-soltan Lake, Iran. The investigated strain was capable of producing carotenoids and protein, using different NaCl concentrations and pH variations in Hoze-soltan saline water. The experiment was carried out within 42 days. The results indicated that protein production and pigmentation correlated well with growth rate. The optimum amount for growth, carotenogenesis, and protein production was determined in a culture with 10% NaCl and pH 8.5. After 42-days incubation, 14.95 ± 0.6 μg/mL of carotenoids and 186 ± 8.6 μg/mL of protein were obtained in this medium. Further observation revealed that growth was inhibited in all samples with 30% NaCl. The amount of each biosynthesized component by Dunaliella salina was in accordance with growth stage, cell number, and growth pattern of microalgae. 相似文献
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