Toxicity of lead nitrate to algae

The fixation of radioactive C was used to mesaure the toxicity of Pb(NO₃)₂ to five species of freshwater algae. Portions of unialgal cultures were inoculated into low salt medium and were used to test all species at 10, 20 and 30 ppm Pb. This medium approximated the salt concentrations of natural aquatic environments. Three different cell weights were used for each concentration of Pb and for the control to determine a relationship between cell weight and toxicity. The concentration of Pb causing a 50% reduction of¹⁴CO₂ fixation as compared to the control was called the ED₅₀ (median effective dose). These values were extrapolated from graphs of ppm Pb vs dpm mg^?1 dry cell weight. The ED₅₀ for three of the species tested (Anabaena, Chlamydomonas andNavicula) was between 15 and 18 ppm Pb. A desmid,Cosmarium, had an ED₅₀ of 5 ppm. This species has a higher surface: volume ratio than the other species tested and this may account for its increased sensitivity. An ED₅₀ forOchromonas was not obtained. Throughout this experiment the fixation of¹⁴CO₂ increased with increasing Pb concentrations and is not readily explained.     

Influence of various fertilization on the mineralogical composition of clay fractions in long-term field experiments

Soil samples from several long-term experiments in the German Democratic Republic (GDR) were taken from plots with and without K-supply. The fine, medium and coarse clay fractions were isolated using ultrasonic dispersion and sedimentation techniques and then investigated by x-ray. Comparisons between the mineralogical compositions found and the results of x-ray analyses of clay fractions from untreated Ah-horizons of the same sites point to accelerated mineral transformations in unfertilized plots. Reductions in the illite contents are proved using the x-ray diffraction diagrams. In connection with the question of the transformational products of this accelerated illite weathering, a new possibility for evaluation by curve-fitting Lp-corrected x-ray diffraction diagrams was tested on one example. The degradation of the illites is partly accompanied with drastic reductions in the amounts of Ca-lactate soluble and adsorbed K. Organic fertilization with 10 to 15 tons of farmyard manure per hectare per year prevented these negative mineralogical changes.     

Seasonal variation of soil respiration in a Pinus cembra forest at the upper timberline in the Central Austrian Alps

Soil respiration (R) of a 95-year-old Pinus cembra L. forest at the alpine timberline was measured continuously from October 2001 to January 2003 with an automated multiplexing gas exchange system. There was significant spatial variability in soil respiration, and R at a soil temperature of 10 degrees C (R10) decreased by about 20% m(-1) with increasing distance from the trunk. Needle litter and fine root density also decreased. The spatially averaged annual soil CO2 efflux was 35 g C m(-2) year(-1) in 2002. About 70% of the temporal variation in soil respiration could be explained by variations in soil temperature, whereas the influence of soil water potential and thus soil water content was negligible because soil water availability was supra-optimal.     

Correlation between corneal sensitivity and quantity of reflex tearing in cows,horses, goats,sheep, dogs,cats, rabbits,and guinea pigs

Objective Guinea pigs have a very low threshold of corneal sensitivity and at the same time nearly no reflex tearing compared to dogs, cats, and horses. The question arose whether there is a general correlation between corneal sensitivity and the quantity of reflex tearing. Animals studied Totally 160 animals of 8 different species (20 animals per species) were investigated. Procedures The corneal touch threshold (CTT) was measured with a Cochet–Bonnet esthesiometer. The palpebral fissure length (PFL) was measured with a calliper ruler. The Schirmer tear test (STT) was modified by adapting the width of the STT strip to the PFL of every species. For the STT II, 0.4% oxybuprocaine was applied. Results Corneal touch threshold: Cows (1.67 g/mm²), horses (1.23 g/mm²), sheep (1.13 g/mm²), goats (1.44 g/mm²), dogs (2.16 g/mm²), and cats (1.33 g/mm²) show similar CTT values. In contrast, rabbits (6.21 g/mm²) and guinea pigs (7.75 g/mm²) show a significantly lower CTT. Tear Production Difference STT I ? STT II: Rabbits have the greatest decline in tear production with 38.4%, followed by sheep (33.3%), dogs (31.1%), cats (24.7%), cows (23.7%), horses (18.0%), and goats (14.0%). Guinea pigs have no decline, but a slight increase of ?16.0%. Correlation CTT and STT II ? STT I Difference: Pearson’s correlation coefficient shows a small, but significant correlation. The coefficient of determination can only forecast a value with 7.1% certainty. Conclusions The high variance and low reproducibility of results suggest that the measuring devices are inappropriate to assess the evaluated parameters. Therefore, no assured correlation between the corneal sensitivity and the quantity of reflex tearing could be found.     

The carbon budget of an adult <Emphasis Type="Italic">Pinus cembra</Emphasis> tree at the alpine timberline in the Central Austrian Alps

We investigated carbon (C) uptake and respiratory losses of an adult Pinus cembra tree at the alpine timberline throughout an entire year by means of an automated, multiplexing gas exchange system. These chamber measurements were then combined with biomass data for scaling up the C budget to the tree level. Integrated over an entire year, the cumulative C gain of the tree under study was 23.5 kg of C in 2002. The daily C balance was negative for 5 months and the estimated total wintertime respiratory losses were 9% of the amount of C fixed during the growing season. The total annual C loss of the tree consumed 55% of the annual net C gain and the remaining surplus was stored in new tissues (36%) and used for fine root growth (9%). Thus, the overall C budget of P. cembra at the upper timberline is balanced fairly well, although the C sink strength in fine roots is strongly limited owing to low root zone temperatures when compared to conifers at lower elevation sites.     

Influence of different copper and zinc supply on their concentrations in blood serum, liver and hair of dairy cows

In a feeding trial at the Institute of Animal Nutrition of the Federal Research Institute of Animal Health (FLI) over 12 weeks with 20 cows of the German Holstein Breed the influence of different copper and zinc contents in the ration on their concentration in blood serum, liver and hair was tested. All animals received a diet based on maize- and grass silage ad libitum. The animals were divided in two groups with 10 cows each; group A received a concentrate according to their milk yield with a copper and zinc content as recommended (GfE 2001), whereas group B was offered a concentrate with roughly the double amount of copper and zinc. At the beginning and at the end of the trial a sample of blood, pigmented hair and a liver bioptate was taken from all animals to evaluate the incorporation of copper and zinc in these tissues. In serum and pigmented hair the copper concentrations did not differ between the two groups [13.4 for Group A and 12.5 micromol/L for Group B in serum respectively 6.8 (Group A) and 7.4 mg/kg DM (Group B) in pigmented hair]. Only the copper concentration in the liver was influenced by the different feeding. The higher copper content for group B resulted in a significantly higher copper concentration in the liver (506 mg/kg DM compared to 383 mg/kg DM). The liver is the best indicator organ for a sufficient copper supply. An increase in the zinc content in the ration resulted neither in higher zinc concentrations in serum (15.1 in Group B in comparison to 13.4 micromol/L for Group A) nor in higher zinc concentrations in liver (140 for Group B and 112 mg/kg DM for Group A) and pigmented hair (130 in Group A and 123 mg/kg DM in Group B). There is a significant correlation between copper intake and copper concentration in the liver (r = 0.46), whereas the correlation between zinc intake and zinc concentration in the liver is only tendencially (r = 0.23). The three tested samples serum, liver and cow hair are not qualified to reflect exactly a sufficient zinc supply.     

Household and farm attributes affecting adoption of smallholder timber management practices by tree growers in Gunungkidul region,Indonesia

Farm household characteristics determine the success of programs promoting agroforestry systems and practices. This paper reports household and farm factors affecting the adoption of timber management practices by smallholders in the Gunungkidul region, Central Java, Indonesia. The research used three logistic regression analysis models—based on each household and farm characteristic, and a composite of both together—to identify the key factors influencing farmers’ adoption and management decisions, and their relative importance. A sample of 152 farmers who managed their trees primarily for timber production was compared to a sample of 115 farmers with similar socio-economic characteristics who did not. The household condition and composite models identified both on-farm and off-farm gross incomes as significant factors affecting farmers’ decisions to manage timber trees. The models confirmed that farmers with larger farms, and with higher on- and off-farm incomes, were more likely to manage their trees for timber production. These results have implications for extension programs that promote adoption of commercial timber management by smallholders in the case study and similar regions.     

Breeding for organic agriculture: the example of winter wheat in Austria

Breeding for organic agriculture (BFOA) is a strategy for a commercial breeding company based on the exploitation of the frequently observed high correlation for many traits between conventional, low input (LI) and organic agriculture (ORG). Indirect selection under LI can be useful to roughly divide the germplasm into the genotypes better adapted to high input and those better adapted to LI or ORG conditions. BFOA is an evolving process, in which two methods are currently applied: early generations are either selected following the pedigree system under LI, or grown as bulk populations on ORG fields. In the latter case the system switches to LI after individual ear selection under ORG conditions. In both methods, the first replicated yield trial is grown parallel under ORG and LI. Subsequently, the genotypes are grouped into conventional or ORG advanced trial series. The BFOA strategy allows that the larger genetic variability of both the organic and conventional gene pool can be exploited in the selection for ORG. Hitherto, seven winter wheat varieties were released in Austria after exclusive organic VCU testing.