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Asmâa Kabbaj Fatiha Zeboudj Didier Peltier Abderrahmane Tagmount Michel Tersac Hubert Dulieu André Bervillé 《Genetic Resources and Crop Evolution》1995,42(4):311-325
Summary Seven wildPetunia species including 2n = 18 species (P. parviflora Jussieu,P. linearis Hook.) and those with 2n = 14 (P. parodii Steene,P. axillaris Lam.,P. integrifolia Hook.,P. inflata R.E. Fries,P. violacea Lindl.) and tenPetunia hybrida horticultural lines were compared for polymorphisms in rDNA genes using the four restriction enzymesEcoRI,BamHI,HindIII andXhoI. All the unit types found in the lines pre-existed in the wild forms. There are two different sizes of either 11.45 or 11.6
kb./The 2n = 18 species are closely related to the 2n = 14 species, thus making thePetunia genus homogeneous. Moreover, it is likely thatP. hybrida lines originated in several kinds of crosses between these species. We constructed a dendrogram for all the 15 rDNA unit
types found. Two main branches of the tree result from the presence or the absence ofHindIII sites. The main branch is divided according to variability at theEcoRI andBamHI sites. Taking into account the existence of several loci which carry one unit type only, we consider whether or not exchanges
might occur between loci. Lines carrying two unit types and lines carrying three unit types support such a hypothesis.XhoI andBamHI fragments enable us to distinguish two types of 5S DNA corresponding to 2n = 18 and 2n = 14 species, respectively.P. hybrida lines and each 2n = 14 wild species carry one of the types only, that corresponds to one 5S DNA locus. The most parsimonious phylogenetic trees
whatever the species chosen as the outgroup, do not fit with our knowledge ofPetunia and with taxonomy. This is likely because only few loci formed the basis of these phylogenetic constructions. 相似文献
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Pathogenicity of Lecanicillium muscarium, against Eretmocerus sp. nr. furuhashii (Hymenoptera: Aphelinidae), was investigated under laboratory conditions to determine if the fungal infection of the whitefly
host can effect the survival, longevity and fecundity of female parasitoid. The results indicated that the number of parasitized
larvae surviving a L. muscarium treatment after 6 days of oviposition decreased with increasing concentrations of L. muscarium and in later stages of development (12 days post oviposition) were not affected by fungal application. There were no significant
differences on adult parasitoid survivorship after 7 days among all treatments. Maximum survivorship (73.33%) was observed
for control and it was minimum (60%) at 1 × 108 conidia/ml. L. muscarium showed a non significant effect on longevity and next offsprings of female parasitoids. The percentage emergence of parasitoids
from the whitefly nymphs produced by the females emerged from treated pupae was almost similar. Maximum emergence (69.77%)
was observed at 1 × 106 conidia/ml and it was lowest (61.02%) at conidial concentration of 1 × 108 conidia/ml. Maximum longevity of adult Eretmocerus sp. emerging from whitefly nymphs when treated after 12 days of postoviposition was observed for 1 × 105 conidia/ml having a mean value of 5 days whereas the lowest longevity was 4.9 days observed at 1 × 108 conidia/ml. The results mentioned above indicate that the interaction among biocontrol agents is positive to a greater extent
with minimum risk hazards. 相似文献
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