Partial colonic obstruction following ovariohysterectomy: a report of three cases

Partial extramural obstruction of the descending colon was diagnosed in two dogs and a cat as a complication of elective ovariohysterectomy. In each case, the obstruction was caused by fibrous tissue that encircled or crossed the descending colon, severely restricting the organ's normal mobility and luminal diameter. Clinical signs secondary to obstipation were observed in two cases, five weeks and 27 months after elective ovariohysterectomy. In one dog without clinical signs, the adhesion was an incidental finding during a laparotomy performed nine years after the ovariohysterectomy. The fibrous adhesions were removed surgically in all three cases without additional complications.     

Molting,Mortality, and the Effects of Density in a Soft-Shell Crawfish Culture System 1

The molting and mortality patterns at a crawfish density recommended for commercial-scale soft-shell crawfish culture systems showed that, over a 116 day period, 74% of the crawfish molted, 22% died, and 4% remained in the system at the end of the study. Approximately three weeks of adaption to the system were required before molting rates of the crawfish began to increase. By the sixth week, the number of malted crawfish per day exceeded the overall mean and remained at this level until 10 days before moltable crawfish were depleted. In a second study, three density levels (3.7,4.9, and 6.1 kg crawfish/m²) were tested for 60 days. The lowest density produced significantly fewer (P < 0.05) molted crawfish. Mortality was significantly greater (P < 0.05) at the highest density compared with the lowest density. The highest density (6.1 kg crawfish/m²) showed a higher molting rate than the lower densities, but it was not significantly different (P < 0.05) from the molting rate of 4.9 kg/m².     

Phosphorus requirements of poultry. IV. The effects on growing pullets of feeding diets containing no animal protein or supplementary phosphorus

Pullets reared to 18 weeks of age on diets containing no animal protein supplements and to which no source of inorganic phosphorus was added showed no clinical symptoms of rickets and no significant differences in average body weight, feed consumption, efficiency of feed conversion or livability compared with pullets reared on comparable diets supplemented with inorganic phosphorus.

The results indicate that growing pullets are able to use effectively organic sources of dietary phosphorus for growth and bone formation and can be reared satisfactorily on starter diets containing lower levels of total phosphorus than currently recommended.

Replacing protein of animal origin in pullet starter and growers' diets by soyabean meal and DL‐methionine or by extracted soyabean meal and extracted sunflower seed meal did not result in any adverse effects.     

Phosphorus requirements of poultry. V. The effects during the subsequent laying year of feeding growing diets containing no animal protein or supplementary phosphorus

Pullets reared to 18 weeks of age on diets containing no feeding stuffs of animal origin and without phosphorus supplementation showed no significant difference in egg yield compared with similar pullets fed rearing diets containing animal protein and added phosphorus.

During the laying stage, diets in commercial use at this centre gave significantly higher egg yields, better average egg prices due to superior grading results and a higher margin over food cost than the same diets modified to conform with the nutrient recommendations of the National Research Council of America (1960).

Where the supplementary protein in both types of layers' diet was of vegetable origin, egg yield, food cost and margin of income over food cost were all significantly inferior to groups fed comparable diets containing supplementary protein of both vegetable and animal origin.

Soyabean meal and sunflower seed meal gave significantly poorer results when used in combination as the sole protein supplement compared with soyabean meal plus methionine.     

Rapamycin-induced insulin resistance is mediated by mTORC2 loss and uncoupled from longevity

Rapamycin, an inhibitor of mechanistic target of rapamycin complex 1 (mTORC1), extends the life spans of yeast, flies, and mice. Calorie restriction, which increases life span and insulin sensitivity, is proposed to function by inhibition of mTORC1, yet paradoxically, chronic administration of rapamycin substantially impairs glucose tolerance and insulin action. We demonstrate that rapamycin disrupted a second mTOR complex, mTORC2, in vivo and that mTORC2 was required for the insulin-mediated suppression of hepatic gluconeogenesis. Further, decreased mTORC1 signaling was sufficient to extend life span independently from changes in glucose homeostasis, as female mice heterozygous for both mTOR and mLST8 exhibited decreased mTORC1 activity and extended life span but had normal glucose tolerance and insulin sensitivity. Thus, mTORC2 disruption is an important mediator of the effects of rapamycin in vivo.