Histological and immunohistochemical features of cutaneous mast cell tumor in six captive four-toed hedgehogs (Atelerix albiventris)

This report described the histopathological and immunohistochemical features of cutaneous mast cell tumor (MCT) in six hedgehogs. The hedgehogs presented single cutaneous mass with ulcer and crusting. Histologically, the neoplastic lesions were characterized by the proliferation of well-differentiated mast cells (3 cases), and atypical mast cells (3 cases) with one atypical histiocytic morphology. Immunohistochemically, tumor cells were positive for KIT and mast cell tryptase, and were negative for Iba-1. In well-differentiated MCT, all patients were clinically improved and survived more than 365 days after surgical excision, whereas an atypical histiocytic MCT showed aggressive behavior with re-recurrence, and the animal died 115 days after surgery. These findings suggest that, compatible with other animals, well-differentiated MCT has a better prognosis in hedgehogs.     

Should only females of giant freshwater prawn Macrobrachium rosenbergii be selected in genetic improvement programmes?

Sexual dimorphism is widely observed in almost all farmed aquatic animal species but giant freshwater pawn (GFP) is unique, with males characterized by three main morphotypes (blue claw, orange claw and small males) and females by different reproduction status (ovary, berried egg and already‐spawned females). There has been reported evidence that the effect of male morphotype may have masked genetic variation in growth‐related traits, as a result the heritability for male body weight was lower than that estimated in female. A pending question has arisen whether selection should be made in female only. To answer this question, we used an 8‐year data set from a long‐term selection programme (2008–2015) for high growth in this species comprising 106,756 individuals that were offspring of 515 sires and 810 dams. The body weight data of female and male GFP or of each morphotype was treated as a separate trait and a multi‐trait approach was used to estimate genetic correlations for homologous traits between sexes and between morphotypes. Our analysis showed that there were little differences in the heritability estimates between female and male. In female, mature ovary individual displayed higher heritability than berried egg and already‐spawned females. For male, the heritability for blue claw, orange claw and small males were 0.11, 0.06 and 0.00 respectively. Between‐sex genetic correlation was moderate (0.55 ± 0.11) for body weight, suggesting that the trait expressions in female and male may be genetically different. In female, the genetic correlations for body weight among three female types were close to one (0.91–0.94). In contrast, the genetic correlations for body weight between male morphotypes especially between blue claw or orange claw and small males were low (0.15–0.25). Furthermore, we estimated genetic gain as the difference in least square means (LSM) or estimated breeding values (EBV) between the selection line and control group. The genetic gain in body weight was smaller in females than in males. It is concluded that there is no need to run separate breeding programme for female and male GFP. A combined selection using both female and male data can achieve selection response for body weight as demonstrated in the present study.     

Use of urea-molasses-multinutrient block and urea-treated rice straw for improving dairy cattle productivity in Vietnam

After conducting a preliminary survey, a feeding trial was carried out to determine the effect of urea-molasses-multinutrient block (UMMB) and urea-treated rice straw (UTRS) as a feed supplement on the productivity of dairy cows. Sixty Holstein-Friesian crossbred cows on 11 smallholder farms were divided equally into control, UMMB and UTRS supplementation groups. Milk yield and feed intake were recorded daily. Milk fat content, body weight and body condition score (BSC) of each cow were determined at two week intervals. Milk samples for progesterone analysis were collected once a week commencing one month after parturition. Data were recorded for date of onset of ovarian activity, estrus, insemination, and conception rate. Milk production increased by 10.3-11.9% and milk fat content increased by 3-5%, therefore, profit for farmers increased by US $0.55-0.73 per cow per day (exchange rate US $1 = VN $11,000). The intervals from calving to onset of ovarian activity (91-94 days), to estrus (110-114 days), to conception (121-122 days) and the calving interval (13.4-13.6 months) in the trial groups were significantly shorter than those in the control group (112, 135, 152 days and 14.4 months, respectively.     

Development of nursery culture techniques for the mud crab Scylla paramamosain (Estampador)

Cannibalism is one of the main causes of mortality in the culture of the mud crab Scylla paramamosain, particularly in the early post‐larval and juvenile stages when the densities of hatchery‐reared crabs may be very high before stocking into ponds or release into the wild for stock enhancement. In a series of experiments investigating cannibalism mitigation, the influence of stocking density, the effectiveness of sand substrate, brick and shell shelters and feed type were compared in culture of crabs from instar 1 for short nursery periods of 15–30 days. Inclusion of brick and shell shelters significantly increased survival over sand substrate alone. However, inclusion of shelters did not affect growth rates. In scaled‐up nursery production in lined‐ponds, with shelters, live Artemia biomass and fresh chopped shrimp or tilapia were found to be equally effective feeds for juvenile crabs stocked at a density of 70 m⁻² from instar 1 and grown for 30 days [52–66% survival, 21.6–24.6 mm carapace width (CW)]. In an extended nursery period for a further 30 days, crabs of 22 mm CW, stocked at 30 m⁻² in the same ponds, attained a final size of 34.5–36.2 mm CW with a survival of 64.3–67.0% using the same feeds.     

Main Characteristics of Statistical Data and the Statistical System for Wood and Wood-processing Products in Vietnam

This paper describes the characteristics of data about wood and wood-processing products published in Vietnam. The characteristics include inconsistency of data published by major data sources, lack of necessary data, and irregular publication frequency. Reasons are identified for the existence of the above characteristics of data for the wood and wood-processing industries, including recent changes in the functions of state statistical organizations and unclear concepts of data published. These characteristics create difficulties for researchers and policy-makers working at the Ministry of Agriculture and Rural Development for analysing policies and establishing supply targets for the wood and wood-processing industries in 5-year economic plans. To improve the statistical system, co-operation between state organisations and the information network (e.g. computers and internet connection) requires strengthening.     

Detection and molecular characterization of sugarcane grassy shoot phytoplasma in Vietnam

Sugarcane is an important cash crop in Vietnam and has been widely promoted at national and provincial level. In 2006, a new disease was discovered in sugarcane in the Nghean Tate&Lyle Sugar Mill in Nghean Province in north-central Vietnam. The key symptoms were the formation of green grassy shoots around the base of mature stools. We applied nested polymerase chain reaction (PCR) using P1/P7 and R16F2n/R16R2 for detection and characterization of phytoplasma from the symptomatic tissues. PCR products of the expected size (approx. 1200?bp) were obtained from the 16S rDNA of the phytoplasma. The restriction fragment length polymorphism profiles indicated that all samples were infected by the same phytoplasma. Phylogenetic analysis confirmed that the SCGS phytoplasma from Vietnam belong to the 16SrXI group, formerly Rice Yellow Dwarf group.