Chlorophyll fluorescence characteristics throughout spring triticale development stages as affected by fertilization

Abstract

Little is known about the response of spring triticale (× Triticosecale Wittm.) chlorophyll fluorescence parameters (Fo – initial fluorescence intensity, Fm – maximal fluorescence, Fv/Fm – maximum quantum yield of photosystem II) to nitrogen fertilization. We investigated after-effects of basic (N₆₀P₆₀K₆₀ and N₁₂₀P₁₂₀K₁₂₀) and additional (N₃₀ and N₆₀) fertilization on chlorophyll fluorescence characteristics at four main phonological development stages of spring triticale under field conditions. Both additional nitrogen (N) rates soon after application induced short-acting stress on both basic fertilization backgrounds only in dry weather conditions. Initial fluorescence intensity exhibited direct relationship (R²=0.52) with an additional nitrogen rate only at the beginning of booting (BBCH 31) in dry 2008. The correlation of maximal fluorescence with additional nitrogen rate was significant during the whole experimental period. Maximum quantum yield of photosystem II directly correlated with both basic and additional nitrogen fertilization from flag leaf stage (BBCH 37) to completion of flowering (BBCH 69) in 2008. Initial fluorescence intensity decreased after spring triticale heading stage.     

Photosynthetic responses of spring barley varieties to different stand densities under field conditions

Abstract

Photosynthesis is affected by many factors on which plant productivity depends; however, little is known about the variation of spring barley photosynthetic characteristics under the influence of different stand densities. A 2-year field study was conducted to determine the photosynthetic rate (A), efficiency of excitation capture by open PSII reaction centres (F'v/F'm) and chlorophyll index (SPAD) response of various spring barley varieties to different stand densities (SDs) under field conditions. Three seed rates were used (200, 400 and 600 plants m^?2) and three varieties (Aura DS, Barke and Gustav). The measurements were made three times during the growing season. Varietal effect was significant on all indicators, the influence of the factors' interaction was significant on A, but SD influence was not significant. Varieties differed in tolerance to weather conditions. Maximum A was established for Gustav in wet year, but for Barke in dry year. The highest F'v/F'm was observed for Gustav under dry and warm conditions. The lowest F'v/F'm across varieties was for the lowest SD. The highest SPAD was observed for Gustav, the lowest for Aura DS. SPAD increased with decreasing SD. The correlation between the indicators and grain yield was positive. Meteorological conditions influenced the A data variation by 46.4–82.5%, 60.9–87.8% and 61.9–83.9% for the Aura DS, Barke and Gustav, respectively. Our research showed that under Central Lithuania's weather conditions the most effective photosynthetic process was recorded in the intensive variety Gustav, which is recommended to be grown at a medium crop density of 400 plants m^?2.     

Use of Ultrasound to Investigate Glucose Oxidase and Storage Effects on the Rheological Properties of Cooked Asian Noodles

This is the first use of a longitudinal ultrasonic technique to address the rheological properties of cooked noodles. Ultrasound (11 MHz) was utilized to investigate the influence of glucose oxidase (GOx) at the 1.5 U/g of flour level on the rheological properties of cooked alkaline noodles before and after 72 h of storage at 4°C. Cooked noodle dough samples were studied by simultaneously conducting stress relaxation and transmission ultrasonic measurements, yielding Peleg's K₁ and K₂ parameters (initial rate of relaxation and extent of relaxation, respectively) and ultrasonic information on noodle texture properties. Ultrasonic phase velocities and attenuation coefficients did not show significant differences between control and GOx noodles either before or after 72 h of refrigeration. However, refrigerated storage of control and GOx noodles did result in a significant increase in wave velocity and storage modulus (M′) as well as a decrease in attenuation and tanδ_L (ratio of longitudinal loss modulus to longitudinal storage modulus), indicating increased firmness of noodle structure with storage time. Stress relaxation results on fresh unrefrigerated noodles showed an increase in Peleg's K₁ and K₂ parameters with GOx addition but did not resolve any significant changes in these parameters after 72 h of storage. This small amount of GOx did not improve cooked noodle texture, although noodle matrix changes during storage were clearly revealed by the noninvasive ultrasonic data.     

Soil sustainability changes in organic crop rotations with diverse crop species and the share of legumes

There have been few long-term field studies on greenhouse gases measurement in organic crop rotations under temperate climatic conditions. Little is known about the extent to which the share of legumes in a crop rotation of organic farming affects the potentials for CO₂ emission and soil organic carbon sequestration. The current study was aimed to investigate soil physicochemical state and soil net CO₂ exchange rate in diverse organic crop rotations with different crop species and proportions of legumes. Four 5-year duration crop rotations were investigated. The best soil sustainability of the arable layer was found in a crop rotation enriched with red clover (Trifolium pratense L.). This rotation resulted in the highest soil mesoporosity and the lowest microporosity, ensured the best supply of plant-available water and revealed high soil resistance to dry conditions. Red clover secured the highest soil organic C sequestration, caused the increase in reserves of total N and available K, and slackened the decrease of soil-available P sources. Red clover-based cropping system exhibited the highest soil net CO₂ exchange rate during five experimental years. The effect of crop rotation, consisting of phacelia (Phacelia tanacetifolia Benth.), peas (Pisum sativum L.) and yellow lupin (Lupinus luteus L.), on soil sustainability was weaker than the effect of rotation with red clover. Non-legume rotations, i.e. binary (two-crop) rotation and the crop rotation involving four spring and one winter species, can be regarded as miners of soil nutrient resources rather than contributors. These rotations did not promote soil sustainability because the soil lost large amounts of macronutrients and caused 26–33% lower soil net CO₂ exchange rate, compared with leguminous rotations. For future, it could be recommended for ecological farming to rely more on crop rotations with red clover to improve ecosystems functioning.     

Relationship between spring triticale physiological traits and productivity changes as affected by different N rates

There is still a lack of knowledge about the physiological traits of spring triticale (x Triticosecale Wittm.) and their relationship with grain yield and protein content under the conditions of the environmental Zone Nemoral 2. The objective of this study was to determine the relationships among the physiological indices, grain yield and protein content as affected by nitrogen (N) rates. The correlation among leaf area index (LAI), chlorophyll index (SPAD), canopy greenness index (CGI), leaf area duration (LAD) and grain yield as well as direct and indirect effects of those traits on the yield were investigated using a path analysis. Grain yield, protein content and physiological indices were significantly (P?≤?.01) affected by N fertilization. N₉₀ level was the best compromise for the yield and physiological indices. The interaction of all physiological indices influenced the grain yield by 27–39%, protein by 42–44%. SPAD and LAI had greater influence on grain yield and grain protein than CGI and LAD. SPAD had positive direct dominant (the highest) effect on the yield only at BBCH 59 and BBCH 69 (50% of the tested cases). LAI was responsible for 19–39% of the correlation between yield and physiological indices. The physiological indices can be used for spring triticale growth modelling and agronomic management for improved productivity and grain quality. SPAD and LAI values, established at BBCH 45–69, can be used for grain yield prediction and those estimated at BBCH 69 can be used for grain protein prediction.

Abbreviations BBCH: Biologische Bundesantalt, Bundessortenamt und Chemische Industrie (decimal system for a uniform coding of phenologically similar growth stages of all mono- and dicotyledonous plant species); CGI: canopy greenness index; GS: growth stage; LAD: leaf area duration; LAI: leaf area index; SPAD: chlorophyll index (soil plant analysis development)     

Virulence of the invasive ash pathogen Hymenoscyphus fraxineus in old and recently established populations

Following its introduction from Asia in the 1990s, the ascomycete Hymenoscyphus fraxineus has caused a severe dieback of Fraxinus excelsior in Europe. In this study, the virulence of 200 H. fraxineus isolates were assessed and compared. These isolates equally represented (i) two geographically distant populations with a different disease history (Switzerland, recently established populations at the epidemic front versus Lithuania, old established populations), and (ii) isolates from two different types of host tissue (necrotic bark lesions as dead‐end tissue versus fallen leaf petioles as primary host tissue). Inoculations conducted on 3‐year‐old F. excelsior seedlings showed that the vast majority of the isolates (98%) were able to induce necrotic bark lesions after 10 months. Although a high variation in virulence was observed among isolates, no significant differences were detected between the older and the epidemic‐front populations. Decline in virulence of populations of invasive organisms is generally assumed with increasing age of epidemics. However, this does not appear to hold true for H. fraxineus. Either the Lithuanian population is still too young (15 to 20 years old) to show a decline in virulence, or the size of the host population may still not be critical for pathogen survival. Given that bark lesions represent an epidemiological dead end and do not benefit the survival of H. fraxineus, a trend towards reduced ‘bark virulence’ of isolates originating from leaf petioles compared to isolates from the bark lesions was expected. However, such a trend was observed neither in old, nor in recently established populations.     

Comparative response of spring and winter triticale productivity and bioethanol yield to fertilisation intensity

Grains of triticale are one of the feedstocks suitable for bioethanol production because they are characterised by high starch and low protein contents. In the present study, spring and winter triticale were comparatively studied to evaluate the influence of N fertilisation intensity on the productivity and bioethanol yield, as well as to assess the relationship between the meteorological factors and ethanol yield. Six treatments of N – 0, 60, 90, 120, 150, and 180?kg?ha^?1 were compared in spring triticale and in winter triticale crops. The analysis of variance showed that nitrogen level (factor A), year (factor B) and their interaction (A × B) significantly (P?≤?.01) influenced grain yield, starch yield and bioethanol yield of both spring and winter triticale. Fertilisation was the main factor explaining 47.6% and 41.0% of the total variability of bioethanol yield of spring and winter triticale, respectively. Nitrogen fertiliser rates 120–180?kg?ha^?1 resulted in maximum bioethanol yield of spring triticale (2417–2480?l?ha^?1) and winter triticale (4311–4420?l?ha^?1). Bioethanol conversion efficiency of nitrogen-fertilised spring and winter triticale was similar 492?l?t^?1 and 508?l?^?1, respectively. Meteorological factors had a greater impact on grain productivity and bioethanol yield for winter triticale than for spring triticale. Both seasonal types of triticale could be good feedstocks for bioethanol production in the areas with congenial weather conditions for their cultivation.     

Forest self-regeneration following clear-felling of dieback-affected Fraxinus excelsior: focus on ash

Due to the dieback caused by invasive fungus Hymenoscyphus pseudoalbidus, ash is threatened in many regions of Europe. In order to predict the situation with ash ecosystems, it is important to examine forest regeneration in areas formerly dominated by ash with long disease history and known management. The main aim of the present study was, therefore, to evaluate forest self-regeneration following clear-felling of ash dieback-devastated stands, focusing on ash. In Lithuania, during 2011, a forest regeneration inventory was performed on twenty clear-cuts, initially dominated by stands of ash, but severely damaged by the dieback caused by H. pseudoalbidus and clear-felled 1–10 years previously. In subsequent forest regeneration, grey alder and birch dominated. Ash regeneration was scarce, and its proportion decreased from 40–100 % in pre-dieback stands to 0–21 % in current stands. Of all observed ash trees (n = 775), 53.9 % were diseased, 16.8 % were dead, and only 29.3 % were visually healthy. Moreover, ash was among the slowest growing species, while grey alder, aspen and birch were the fastest. The regeneration and health condition of ash in forests, where previous to the dieback ash was dominant, demonstrated a sharp decrease in occurrence of this species and a clear shift in species composition towards grey alder and birch.