Short-term dietary supplementation with the microalga Parietochloris incisa enhances stress resistance in guppies Poecilia reticulata

Two trials were conducted to determine the effects of dietary enrichments with the microalga Parietochloris incisa , rich in arachidonic acid (ARA), on stress resistance in guppies Poecilia reticulata . The microalga was added to commercial diets as a neutral lipid (NL) extract and its fractions or as broken cells. Experimental diets were applied for a period of 14 days. In trial 1, commercial diets were supplemented with NL (containing 25 mg ARA and 0.11 mg β-carotene g⁻¹ feed), its triacylglycerol (TAG) fraction (containing 25 mg ARA g⁻¹ feed and no β-carotene) and the β-carotene fraction (containing 0.11 mg carotenoid g⁻¹ feed and minute amounts of ARA). Neutral lipid-fed fish demonstrated the highest resistance ( P <0.05) to osmotic stress (32-ppt NaCl), followed by fish fed with diets supplemented with TAG and β-carotene alone, which were more resistant than control ( P <0.05). In trial 2, fish fed diets supplemented with higher levels of broken alga (26.1 mg ARA g⁻¹ feed) were more resistant ( P <0.05) to stress as compared with fish fed lower ARA (16.3 mg g g⁻¹) or an unsupplemented control diet. We suggest a dietary supplementation with broken P. incisa cells to enhance stress resistance in guppies before a stressful event.     

Role of live microbial feed supplements with reference to anaerobic fungi in ruminant productivity: A review

To keep the concept of a safe food supply to the consumers, animal feed industries world over are showing an increasing interest in the direct-fed microbials(DFM) for improved animal performance in terms of growth or productivity. This becomes all the more essential in a situation, where a number of the residues of antibiotics and/or other growth stimulants reach in milk and meat with a number of associated potential risks for the consumers. Hence, in the absence of growth stimulants, a positive manipulation of the rumen microbial ecosystem to enhance the feedstuff utilization for improved production efficiency by ruminants has become of much interest to the researchers and entrepreneurs. A few genera of live microbes(i.e., bacteria, fungi and yeasts in different types of formulations from paste to powder) are infrequently used as DFM for the domestic ruminants. These DFM products are live microbial feed supplements containing naturally occurring microbes in the rumen. Among different DFM possibilities, anaerobic rumen fungi(ARF) based additives have been found to improve ruminant productivity consistently during feeding trials. Administration of ARF during the few trials conducted, led to the increased weight gain, milk production, and total tract digestibility of feed components in ruminants. Anaerobic fungi in the rumen display very strong cell-wall degrading cellulolytic and xylanolytic activities through rhizoid development, resulting in the physical disruption of feed structure paving the way for bacterial action. Significant improvements in the fiber digestibility were found to coincide with increases in ARF in the rumen indicating their role. Most of the researches based on DFM have indicated a positive response in nutrient digestion and methane reducing potential during in vivo and/or in vitro supplementation of ARF as DFM. Therefore, DFM especially ARF will gain popularity but it is necessary that all the strains are thoroughly studied for their beneficial properties to have a confirmed ‘generally regarded as safe' status for ruminants.     

Prospective use of bacteriocinogenic Pediococcus pentosaceus as direct-fed microbial having methane reducing potential

Direct-fed microbials(DFM), generally regarded as safe status, are successfully used in improving rumen ecology, gastro-intestinal health, feed efficiency, milk production and growth rate in ruminants. On the other hand, methanogenesis in rumen, which accounts for a significant loss of ruminant energy and increased greenhouse gas in environment, is of great concern, therefore, use of DFM for improving productivity without compromising the animal health and ecological sustainability is encouraged. The present study was conducted to investigate the methane reducing potential of bacteriocinogenic strain Pediococcus pentosaceus-34. Since, the culture showed no hemolysis on blood agar and DNase activity, hence, it was considered to be avirulent in nature, a prerequisite for any DFM. The culture also showed tolerance to pH 5.0 for 24 h with 0.5% organic acid mixture, whereas when given a shock for 2 h at different p H and organic acids concentrations, it showed growth at pH 3.0 and 4.0 with 0.1 and 1.0% organic acids, respectively, as having good animal probiotics attributes. The total gas production was significantly(P<0.05) higher in live pedicoccal culture(LPC) and dead pedicoccal culture(DPC) both with wheat straw, when compared to the control. In sugarcane bagasse, gas production was significantly lower(P<0.05) with LPC compared to the control and DPC both. Methane was reduced by the inclusion of LPC in sugarcane bagasse(0.07 mL CH4 mg–1 dry matter digestibility) with no effect on other rumen fermentation parameters. However, with wheat straw and LPC total gas, in vitro dry matter digestibility, total volatile fatty acids increased significantly but no reduction in methane production was observed in comparison to the control. Therefore, further research is warranted in this direction, if the bacteriocinogenic strains can be used as DFM for ruminants to improve the ruminant productivity.     

Growing fruit trees in highly alkali soils—a case study

The performance of 10 fruit species, namely pomegranate (Punica granatum), guava (Psidium guajava), sapota (Achras japota), baelpather (Aegle marmelos), amla (Emblica officinalis), ber (Zizyphus mauritiana), karaunda (Carissa carandas), date palm (Phoenix dactyleform), jamun (Syzygium cuminii) and imli (Tamarindus indica), as affected by site preparation and amendment use, was evaluated in a replicated field trial established in 1992 in a highly alkali soil (pH 10·5) at the Bichhian experimental farm of the Central Soil Salinity Research Institute, Karnal. The treatments involved two site preparation methods: (1) augerholes of 20–25 cm diameter and 160–180 cm deep made in the centre of 45 cm×45 cm pits in the main plot and (2) pits of 90 cm×90 cm×90 cm; variable amendments composition in the subplot and fruit species in the sub-subplots. Growth observations recorded 26 months after planting showed that survival, height and girth of all species remained unaffected owing to site preparation techniques and amendment use. Irrespective of planting techniques and amendment use, jamun, guava, ber and imli performed best. Date palm and baelpather performed poorly. Initial growth of sapota was satisfactory, but it was found highly sensitive to frost. Similarly, pomegranate which was performing exceedingly well was found very sensitive to prolonged water stagnation. This 3-year study indicated that out of 10 species tried, about half a dozen fruit plants can be established in alkali soils after following appropriate site preparation methods and better management practices. Established species came to bearing between 18 and 24 months after planting, but the fruits were damaged by prolonged water stagnation during the monsoon season and chilling temperatures of the 1994–95 winter. This study further indicated that the augerhole method of root bed preparation, is an economical, less laborious and faster way of planting fruit trees than is the pit method. The experiment will be continued to study treatment effects on fruit production and quality before making final recommendations. © 1997 John Wiley & Sons, Ltd.     

Forage production and nitrogen nutrition in three grasses under coconut tree shades in the humid-tropics

Reduction in forage production (FP) under trees in the humid tropics is well known, but information on how different levels of nitrogen (N) fertilizer influence FP under trees is meager. The present study reports effects of four N fertilizer levels (0, 60, 80 and 120 kg ha⁻¹ N) on net soil N mineralization rate (NMR) and soil moisture (SM), FP, shoot biomass/root biomass ratio (SB/RB), N concentration in SB, N uptake and nitrogen use efficiency (NUE) of three grasses [guinea (Panicum maximum Jacq.), para (Brachiaria mutica (Forssk) Stapf) and hybrid-napier (Pennisetum purpureum Schumach.)] under three canopy positions [under canopy (UC, representing high shade), between canopy (BC, representing low shade) and open] of coconut trees (Cocos nucifera L.) in a coconut based silvopastoral system in the humid tropical climate of South Andaman Island of India. The study was performed for two annual cycles (2005–2006 and 2006–2007). The hypotheses tested were: (1) FP would decline under tree shades, both in N fertilized as well as no N fertilized conditions, when SM was not growth limiting in the open. However, amount of decline in the FP would depend on grass species and intensity of shades i.e., higher was the shade greater would be the decline; (2) N fertilizer would increase FP under tree shades, but the increase depended on grass species, intensity of shades and amount of N applied. Amount of N applied, however, would not annul the shades effects when SM was not growth limiting in the open. The study revealed that the tree reduced light 59% under UC and 32% under BC positions, but the N fertilizer levels increased NMR by 11–51% under UC and 3–44% under BC positions compared to the open. SM did not differ across the canopy positions. Under all situations, FP of all grasses declined under UC (47–78%) and BC (18–32%) positions compared to the open; the decline was greater in Hybrid-napier than Guinea and Para grasses. Forage production of all grasses increased with N fertilizer increments under all canopy positions reaching 32 t ha⁻¹ dry matters for hybrid-napier at 120 kg ha⁻¹ N in the open. Both guinea and para grasses outyielded hybrid-napier grass under UC but not under BC or in the open. N concentration in the forage (SB) also increased as N fertilizer level increased. These observations support our hypotheses and suggest that forage production under coconut palms can be increased by the application of N fertilizer with both guinea and para grasses being more productive than hybrid-napier grass under the high shade. Where light conditions are better, hybrid-napier would produce more forage than the other species.     

Performance of some under‐explored crops under saline irrigation in a semiarid climate in Northwest India

Growing salt‐tolerant under‐explored crops utilizing saline ground water can provide for an economic use of abandoned semiarid lands. Field trials were conducted between 1999 and 2003 on a calcareous soil in a semiarid region of northwest India. Woody perennials were planted at the sill of furrows and irrigated with water of high salinity (EC 10–28 dS m⁻¹), low salinity (EC 5–9 dS m⁻¹) and alternately with these two waters. Woody species included Azadirachta indica, Cordia rothii, Salvadora persica, Jatropha curcas, J. gossipifolia, Ricinus communis, Catharanthus roseus, Adhatoda vasica and Aloe barbadensis. Most of these could be grown successfully but S. persica—a highly salt‐tolerant halophyte—though it produced huge biomass, could not yield mature fruit due to frost injury. The salinity build up in the soil was greater during low‐rainfall years, but a good rainfall year, e.g. 714 mm in 2001, helped to leach out the accumulated salts. The uptake of Na⁺ in plants was greater when irrigated with water of high salinity, while K⁺ accumulation was greater with water of low salinity. Na⁺ accumulation was higher in roots as compared to other parts except in Jatropha and Salvadora, while K⁺ accumulation was greater in leaves. There was a negative correlation between Na⁺ and K⁺ accumulation and a positive correlation between Ca²⁺ and Mg²⁺. Thus, saline water (ECiw 12 dS m⁻¹) can successfully be used for growing several under‐explored crops of high economic value. Copyright © 2006 John Wiley & Sons, Ltd.     

Role of live microbial feed supplements with reference to anaerobic fungi in ruminant productivity:A review

To keep the concept of a safe food supply to the consumers, animal feed industries world over are showing an increasing interest in the direct-fed microbials (DFM) for improved animal performance in te...     

An evaluation of agriculture, forestry and agroforestry practices in a moderately alkali soil in northwestern India

Productivity, sustainability and economics of agriculture, forestry and agroforestry land use practices were compared over a six year period in a split plot experiment on a moderately alkali soil of the Central Soil Salinity Research Institute, Karnal, India. Three commercial trees of the area formed the main plot treatments and four crop sequences were the sub-plots. The trees were: poplar (Populus deltoides), Acacia (Acacia nilotica) and Eucalyptus (Eucalyptus tereticornis), and the crop sequences were (1) rice (Oryza sativa)-wheat (Triticum aestivum) for four years followed by guinea grass (Panicum maximum)-oats (Avena sativa) for two years; (2) rice-Berseem (Trifolium alexandrium) for four years followed by cowpea (Vigna unquiculata)-Berseem for two years; (3) pigeonpea (Cajanus cajan)/sorghum (Sorghum bicolor)-mustard (Brassica juncea) for three years followed by turmeric (Curcuma longa) for three years and (4) no intercrops (only trees). Eucalyptus and poplar gained maximum height, girth and woody biomass in six years when they were intercropped with rice crops in sequences 1 and 2. Acacia attained maximum growth in the absence of intercrops. Protein content in guinea grass was more under tree canopies than in the open. Soil amelioration during five years followed the order: Acacia based system > poplar > Eucalyptus > sole crops. The benefit-cost ratio was heighest (2.88) in poplar based system and minimum (1.86) in Acacia based system. The study indicated that growing trees and agricultural crops together is a better land use option in terms of productivity, maintenance of soil conditions and economics. This revised version was published online in June 2006 with corrections to the Cover Date.     

Growing three aromatic grasses in different alkali soils in semi‐arid regions of northern India

Alkali lands in India occupy about 3 million ha. Due to poor physical properties, excessive exchangeable sodium and high pH, most of these lands support a very poor vegetation cover. Many of the medicinal and aromatic plants are in great demand for both internal requirements and export. But since these crops are non‐conventional in nature, it is not always possible for them to be produced on fertile lands, which can be used for arable crops. The marginal lands, specifically the lands affected by salinity or sodicity problems where profitable returns are not possible from agricultural crops, could be successfully utilized for the cultivation of these high‐value crops with marginal inputs. Results reported in this paper clearly indicated that out of three aromatic grasses, palmarosa (Cymbopogon martinii (Roxb.) Wats.) and lemon grass (C. flexuosus (Steud.) Wats.) could successfully be grown on moderatly alkali soils of pH up to 9·2 while vetiver (Vetiveria zizanioides (L.) Nash), which withstands both high pH and stagnation of water, could successfully be grown without significant yield reduction on highly alkali soils. These grasses not only produce essential oils used for industrial purposes but also ameliorate the soil. Copyright © 2004 John Wiley & Sons, Ltd.