Toxicosis in sheep following ingestion of natural gas condensate.

Thirty of 200 ewes died or were euthanatized during a 21-day period following a 1-day accidental exposure to natural gas condensate, a complex mixture of hydrocarbons obtained during collection of natural gas from wells. Despite access to potable well water, the poisoned ewes willingly consumed toxic doses of condensate that contaminated surface water. Eight animals died without premonitory signs; the remainder became ill over the course of a few days to 3 weeks. The principal cause of mortality was aspiration pneumonia, but myocardial degeneration and necrosis, renal tubular damage, gastritis, enteritis, and meningeal edema and hyperemia were also observed. Gas chromatographic analysis identified chemical traces of the hydrocarbons in the tissues, and "fingerprinting," the process of matching chromatographic tracings, provided forensic proof of the contamination source. Atomic absorption spectroscopy and cholinesterase analyses were performed to eliminate the possibility of toxicosis by heavy metal contaminants or other constituents. This appears to be the first reported incidence of natural gas condensate toxicity involving sheep or other ruminants. Although the available literature presents a suggestive pattern of clinical signs and pathologic lesions of petroleum product poisoning, diagnostic investigations should employ detailed analytic examination because each source of petroleum hydrocarbons contains unique sets of components that may produce different toxic effects.     

Flowing solution culture system maintains uniform root environment composition

Flowing solution culture enables the researcher to study effects of root environment variables independently. This flowing solution culture system utilizes air pressure and syphoning or a peristaltic pump to circulate the nutrient solution. Because this system is inexpensive, simple in design, and compact, several factors can be studied concurrently, treatments can be replicated to test for system effects, nutrient deviations, and block for growth effects due to environment. These systems have been used successfully with experiments in N form, plant growth regulators, salt stress, and mechanical stress. Non‐destructive measurements of root growth and nutrient uptake are possible.     

Eine Anordnung zur Messung kleiner Sauerstoffaustauschraten photosynthetisierender Algen bei geringen Lichtintensitäten

Zusammenfassung Es wird eine Meßanordnung zur amperometrischen Registrierung der relativen Nettosauerstoffaustauschrate mittels einer membrangeschützten Platinelektrode beschrieben, die besonders für die Verwendung kleiner Versuchsobjekte (einzelliger Algen und isolierter Chloroplasten) geeignet ist. Die hohe Empfindlichkeit ermöglicht die Analyse von Sauer-stoffaustauschraten unter Verwendung monochromatischen Lichtes relativ hoher spektraler Reinheit. Die gute Stabilität des Elektrodenpotentials läßt eine große Verstärkung des Meßwertes und eine direkte Registrierung des Anstiegs der relativen Sauerstoffaustauschrate zu.

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Summary An amperometric device is described for the registration of relative oxygen exchange rates of small particles such as algae or isolated chloroplasts. The high sensitivity of the electrode according to the Clark-type make possible the application of monochromatic light with small half band wides for examination of photosynthetic reactions. The employed Ag/AgO-reference electrode has a very stable potential and allows a high amplification necessary for direct registration of the oxygen exchange rate derivatives. Some examples are given for registrated exchange rates.

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Canine visceral leishmaniasis: dog infectivity to sand flies from non-endemic areas

Canine visceral leishmaniasis (VL), caused by Leishmania infantum (Leishmania chagasi in the New World), is a zoonotic, endemic disease in Western Europe and Latin America. The potential spreading to new regions was suggested by the appearance of canine VL among foxhounds in the US. Although the sand fly vectors in the major foci of transmission have been described, no information exists on other sand flies that could propagate the infection outside endemic areas. We evaluated the capacity of Lutzomyia shannoni (Dyar) and Lutomyia youngi (Feliciangeli & Murillo), which are widely distributed in the New World, to acquire L chagasi (Cunha and Chagas) infections. A high proportion of L youngi were infected after feeding on an oligosymptomatic dog (51 per cent) or a polysymptomatic individual (95 per cent), but the intensity of infection was low (< 200 promastigotes/fly). L shannoni became infected only by feeding on the polysymptomatic dog, and the infection rate was lower (9 per cent) than in Lutzomyia longipalpis (36 per cent), and Lutzomyia evansi (Nunez-Tovar) (Lutz and Neiva) (38 per cent), but the intensity of infection (200 to > 500 promastigotes/fly) was comparable (L longipalpis) or higher (L evansi) than in the New World vectors. It is hypothesised that the presence of infected dogs in areas where L shannoni or L youngi occur could initiate new endemic cycles of VL in both South and North America.     

Enzymatic radioimmunoassay for detecting Leptospira interrogans serovar pomona in the urine of experimentally-infected pigs

An enzymatic radioimmunoassay (ERIA) has been developed for detecting Leptospira interrogans serovar pomona in porcine urine. Four grower pigs were experimentally infected with serovar pomona. A total of 39 urine samples was collected, and ERIA was compared with dark ground microscopy (DGM) and culture for demonstrating leptospiruria. Of 20 samples positive by at least one technique, leptospires were detected by ERIA in 14, by culture in 16 and by DGM in 13. ERIA, unlike the other 2 methods, was suitable for use with urine which had been stored frozen for several months.     

Lignin chemistry—past,present and future

Summary Some pertinent results and views from the earlier history of lignin chemistry, pointing to the importance of the arylpropane skeleton, are outlined. Later development, beginning with the dehydrogenation theory and experimental studies on the dehydrogenative polymerization of p-hydroxycinnamyl alcohols, is then reviewed. Finally, recent degradative work resulting in a detailed picture of lignin structure is discussed.     

Co-application of compost or inorganic NPK fertilizer with biochar influences soil quality,grain yield and net income of rice

Most agricultural soils in sub-Saharan Africa are degraded, compromising the grain yield of rice and farmers return on investment. A 3-year field study was undertaken to explore the effect of the application of compost or inorganic NPK fertilizer applied alone or in combination with biochar on soil quality, grain yield of rice and net income. The five treatments were laid out using a randomized complete block design with four replications. The treatments were applied to supply approximately 75 kg N ha^–1. The best fertilizer input was compost+biochar which resulted in the greatest improvement in soil physico-chemical properties by reducing bulk density and increasing porosity and moisture retention, organic matter content, percent nitrogen, available phosphorus and cation exchange capacity. Apart from treatment with inorganic fertilizer alone, treated soils showed a decrease in pH. Bacterial and fungal counts and basal respiration decreased in soils in the following order: compost+biochar>compost only>inorganic NPK fertilizer+biochar>inorganic NPK fertilizer>control. The increase in pooled grain yield and net income in response to treatment followed the order: compost+biochar>NPK+biochar>NPK>compost>control. The findings suggest that the use of compost or NPK alone might improve soil quality and increase grain yield and net income, but it is greatly recommended to co-apply these fertilisers with biochar.     

In silico prediction of Gallibacterium anatis pan-immunogens

The Gram-negative bacterium Gallibacterium anatis is a major cause of salpingitis and peritonitis in commercial egg-layers, leading to reduced egg production and increased mortality. Unfortunately, widespread multidrug resistance and antigenic diversity makes it difficult to control infections and novel prevention strategies are urgently needed. In this study, a pan-genomic reverse vaccinology (RV) approach was used to identify potential vaccine candidates. Firstly, the genomes of 10 selected Gallibacterium strains were analyzed and proteins selected on the following criteria; predicted surface-exposure or secretion, none or one transmembrane helix (TMH), and presence in six or more of the 10 genomes. In total, 42 proteins were selected. The genes encoding 27 of these proteins were successfully cloned in Escherichia coli and the proteins expressed and purified. To reduce the number of vaccine candidates for in vivo testing, each of the purified recombinant proteins was screened by ELISA for their ability to elicit a significant serological response with serum from chickens that had been infected with G. anatis. Additionally, an in silico prediction of the protective potential was carried out based on a protein property prediction method. Of the 27 proteins, two novel putative immunogens were identified; Gab_1309 and Gab_2312. Moreover, three previously characterized virulence factors; GtxA, FlfA and Gab_2156, were identified. Thus, by combining the pan-genomic RV approach with subsequent in vitro and in silico screening, we have narrowed down the pan-proteome of G. anatis to five vaccine candidates. Importantly, preliminary immunization trials indicated an in vivo protective potential of GtxA-N, FlfA and Gab_1309.

Electronic supplementary material

The online version of this article (doi:10.1186/s13567-014-0080-0) contains supplementary material, which is available to authorized users.