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1.
The cultivation of perennial instead of annual energy crops has received growing interest. Previous studies identified numerous beneficial effects of perennial energy crop (PEC) cultivation for the agricultural landscape such as promotion of agrobiodiversity, reduced requirements for agrochemicals and fertilizers as well as a large potential for carbon accumulation in soil. However, the mere presence of soil organic matter (SOM) accumulation gives no indication about the persistence of the SOM for example after a recultivation of the stands. Therefore, this study focused on SOM pools of different density fractions and soil microbial parameters. Six different PECs were tested against a typical benchmark system as feedstock for anaerobic digestion. The study has shown that all PEC species increased soil microbial activity and provided an insight how they sequester carbon in soil. Moreover, significant modifications in basic soil properties caused by plant growth were observed. For example, the cultivation of giant knotweed has lowered the soil pH by more than 0.5 pH units compared to the benchmark system. After 5 years of PEC cultivation, total soil organic carbon stocks were increased between 1,500 ± 400 and 4,500 ± 1,500 kg C ha-1 for the upper 10 centimetres of soil. The distribution among different soil fractions showed species-specific patterns. Tall wheatgrass and Virginia mallow showed particular high accumulation rates in the mineral-associated SOM fraction which indicates long residence times of the SOM after a possible recultivation of the fields. 相似文献
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Neis C Rohde J Valentin-Weigand P Baums CG 《Berliner und Münchener tier?rztliche Wochenschrift》2007,120(5-6):202-206
Streptococcus (S.) suis plays an important role in pig breeding causing invasive diseases such as meningitis and polyarthritis. Herd problems with Streptococcus suis are common in many pig farms and are frequently characterised by disease outbreaks. In this context, it is often important to identify chains of infection, e.g. between a farrow-to-wean and a grower farm. In the following case report a possible chain of infection among the different farms of a farrow-to-finish system was investigated. In two grower units herd problems with S. suis were present; the mortality was higher than 5 %. It appeared likely, that the streptococci causing these problems were transmitted from a single farrow-to-wean unit to the two different grower farms. In the respective farms swabs were taken from different healthy animals and, in the case of the grower farms, also from the infected animals. Genotypic profiling of strains by a multiplex-PCR and AFLP typing method revealed that two different S. suis pathotypes were responsible for the herd problems. Both pathotypes could not be detected in the farrow-to-wean farm.Thus, a chain of infection originating from the farrow-to-wean farm appeared unlikely. The multiplex PCR was in this case sufficient to elucidate the described problem. 相似文献
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Adolfo Isla Mnica Saldarriaga‐Crdoba Derie E. Fuentes Romina Albornoz Denise Haussmann Jorge Mancilla‐Schulz Alexis Martínez Jaime Figueroa Ruben Avendao‐Herrera Alejandro Yez 《Journal of fish diseases》2019,42(5):721-737
Piscirickettsia salmonisis the causative bacterial pathogen of piscirickettsiosis, a salmonid disease that causes notable mortalities in the worldwide aquaculture industry. Published research describes the phenotypic traits, virulence factors, pathogenicity and antibiotic‐resistance potential for various P. salmonisstrains. However, evolutionary and genetic information is scarce for P. salmonis. The present study used multilocus sequence typing (MLST) to gain insight into the population structure and evolution of P. salmonis. Forty‐two Chilean P. salmonisisolates, as well as the type strain LF‐89T, were recovered from diseased Salmo salar, Oncorhynchus kisutchand Oncorhynchus mykissfrom two Chilean Regions. MLST assessed the loci sequences of dnaK, efp, fumC, glyA, murG, rpoD and trpB. Bioinformatics analyses established the genetic diversity among P. salmonis isolates (H = 0.5810). A total of 23 sequence types (ST) were identified, 53.48% of which were represented by ST1, ST5 and ST2. Population structure analysis through polymorphism patterns showed few polymorphic sites (218 nucleotides from 4,010 bp), while dN/dS ratio analysis indicated purifying selection for dnaK, epf, fumC, murG, and rpoD but neutral selection for the trpB loci. The standardized index of association indicated strong linkage disequilibrium, suggesting clonal population structure. However, recombination events were detected in a group of seven isolates. Findings included genogroups homologous to the LF‐89T and EM‐90 strains, as well as a seven‐isolate hybrid genogroup recovered from both assessed regions (three O. mykiss and four S. salar isolates). The presented MLST scheme has comparative potential, with promising applications in studying distinct P. salmonis isolates (e.g., from different hosts, farms, geographical areas) and in understanding the epidemiology of this pathogen. 相似文献
7.
The growth data of a commercial aquaculture recirculation system were analysed to investigate the growth performance of reared
turbot (Psetta maxima). Three common growth models (von Bertalanffy, Gompertz and Schnute) were fitted to the growth data documented over a time
period of 6 years. To determine the most suitable model, three different criteria were used: (1) the Akaike index criterion,
(2) the sum of squared residuals and (3) the average daily deviation between the estimated final weight and the observed final
weight. The evaluation of the growth models showed that the Schnute model had the lowest Akaike index, the lowest sum of squared
residuals and the lowest daily deviation between estimated and real weight of all tested growth models. The Schnute model
produced sigmoid growth curves. The estimated growth coefficients were the most realistic ones in regard to biological interpretation.
In contrast, the von Bertalanffy growth model and the Gompertz model estimated inaccurate exponential growth curves and are
therefore unable to simulate the growth data as well as the Schnute model. The results indicate that the von Bertalanffy growth
model is not the optimal model to simulate the present growth data and that the growth potential of reared turbot has probably
not yet been fully exploited in the aquaculture system(s) examined (so far). 相似文献
8.
Spermatogenesis and its endocrine regulation 总被引:2,自引:2,他引:2
Three major phases compose spermatogenesis: mitotic proliferation of spermatogonia, meiosis of spermatocytes, and spermiogenesis,
the restructuring of spermatids into flagellated spermatozoa. The process is fuelled by stem cells that, when dividing, either
self-renew or produce spermatogonia that are committed to proliferation, meiosis, and spermiogenesis. During all phases, germ
cells are in close contact with and require the structural and functional support of Sertoli cells. In contrast to germ cells,
these somatic cells express receptors for sex steroids and follicle-stimulating hormone (FSH), the most important hormones
that regulate spermatogenesis. A typical Sertoli cell response to an endocrine stimulus would be to change the release of
a growth factor that would then mediate the hormone's effect to the germ cells. Recent studies in the Japanese eel have shown,
for example, that in the absence of gonadotropin Sertoli cells produce a growth factor (an orthologue of anti-Müllerian hormone)
that restricts stem cell divisions to the self-renewal pathway; also estrogens stimulate stem cell renewal divisions but not
spermatogonial proliferation. Gonadotropin or 11-ketotestosterone (11-KT) stimulation, however, induces spermatogonial proliferation,
which is in part mimicked by another Sertoli cell-derived growth factor (activin B). Since FSH (besides luteinizing hormone,
LH) stimulates steroidogenesis in fish, and since FSH is the only gonadotropin detected in the plasma of sexually immature
salmonids, increased FSH signalling may be sufficient to initiate spermatogenesis by activating both Sertoli cell functions
and 11-KT production. Another important androgen is testosterone (T), which seems to act via feedback mechanisms that can
compromise FSH-dependent signalling or steroidogenesis. The testicular production of T and 11-KT therefore needs to be balanced
adequately. Further research is required to elucidate in what way(s) 11-KT stimulates later stages of development, such as
entry into meiosis and spermiogenesis. At this period, LH becomes increasingly important for the regulation of androgen production.
Results from mammalian models suggest that during the later phases, the control of germ cell apoptosis via Sertoli cell factors
is an important regulatory mechanism. In many species, sperm cells cannot fertilize eggs until having passed a maturation
process known as capacitation, which includes the acquisition of motility. Progestins that are produced under the influence
of LH appear to play an important role in this context, which involves the control of the composition of the seminal plasma
(e.g., pH values).
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
9.
The effects of cytokinins, carbohydrate sources and cold pretreatment on the conversion of protocorm-like bodies (PLBs) to shoots were investigated for the enhancement of micropropagation of Dendrobium huoshanense C.Z. Tang et S.J. Cheng, an endangered medicinal plant in China. The effects of cytokinins and carbohydrate sources on the conversion of PLBs to shoots depended on their types and concentrations. The best results in terms of shoot development from PLBs occurred on 1/2 MS medium supplemented with 20 μM kinetin and 10 g l−1 maltose. Cold pretreatment at 10 °C for 1–2 weeks significantly enhanced the conversion of PLBs to shoots, and over 1300 shoots were obtained from one gram of PLBs after 3 months of culture. The developed shoots were rooted on growth regulator-free MS medium supplemented with 8 g/l banana paste to give complete plantlets, which were successfully acclimatized with a survival rate of approximately 65%. The results indicate that a suitable cold pretreatment (10 °C for 1 week) followed by the use of 20 μM kinetin and 10 g/l maltose in 1/2 MS medium would produce a large number of shoots from PLBs for plantlet regeneration of D. huoshanense. 相似文献
10.
Relation between soil organic matter and yield levels of nonlegume crops in organic and conventional farming systems 总被引:2,自引:0,他引:2
Christopher Brock Andreas Fließbach Hans‐Rudolf Oberholzer Franz Schulz Klaus Wiesinger Frank Reinicke Wernfried Koch Bernhard Pallutt Bärbel Dittman Jörg Zimmer Kurt‐Jürgen Hülsbergen Günter Leithold 《植物养料与土壤学杂志》2011,174(4):568-575
The aim of this study was to evaluate the interaction between yield levels of nonleguminous crops and soil organic matter (SOM) under the specific conditions of organic and conventional farming, respectively, and to identify implications for SOM management in arable farming considering the farming system (organic vs. conventional). For that purpose, correlations between yield levels of nonlegume crops and actual SOM level (Corg, Nt, Chwe, Nhwe) as well as SOM‐level development were examined including primary data from selected treatments of seven long‐term field experiments in Germany and Switzerland. Yield levels of nonlegume crops were positively correlated with SOM levels, but the correlation was significant only under conditions of organic farming, and not with conventional farming treatments. While absolute SOM levels had a positive impact on yield levels of nonlegumes, the yield levels of nonlegumes and SOM‐level development over time correlated negatively. Due to an increased demand of N from SOM mineralization, higher yield levels of nonlegumes obviously indicate an increased demand for OM supply to maintain SOM levels. Since this observation is highly significant for farming without mineral‐N fertilization but not for farming with such fertilization, we conclude that the demand of SOM‐level maintenance or enhancement and thus adequate SOM management is highly relevant for crop production in organic farming both from an agronomical and ecological point of view. Under conventional management, the agronomic relevance of SOM with regard to nutrient supply is much lower than under organic management. However, it has to be considered that we excluded other possible benefits of SOM in our survey that may be highly relevant for conventional farming as well. 相似文献