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Vacek Zdeněk Cukor Jan Vacek Stanislav Linda Rostislav Prokůpková Anna Podrázský Vilém Gallo Josef Vacek Oldřich Šimůnek Václav Drábek Ondřej Hájek Vojtěch Spasić Marko Brichta Jakub 《European Journal of Forest Research》2021,140(5):1243-1266
European Journal of Forest Research - In the time of ongoing climate change and the increasing area of post-mining landscape, the successful afforestation of reclamation sites by suitable adaptive... 相似文献
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Seven hybridoma clones, E2/G2, E2/B5, E4/C2, G5/E10, F6/C10, B5/C3, and B7, produced within one fusion experiment in 1991 and the clone E4/C2 originated from 1995 were characterized by sequencing of genes coding for variable domains of the antibodies against 2,4-D herbicide. Amino acid sequences of selected antibodies, deduced from DNA analysis, were confirmed by mass spectrometry. Surprisingly, nucleotide sequence analysis of the clones E2/G2 and E2/B5, producing the most sensitive antibodies, proved to have sequence homology of their variable domains, although the IC(50) values determined for these antibodies 9 years prior to the DNA analysis were 2.0 and 8.2 ng/mL, respectively. The same findings arose from the comparison of the immunochemical to DNA data established for G5/E10, F6/C10, and B5/C3 clones producing antibodies with IC(50) values in the range of 26.3-43.1 ng /mL. The clone E4/C2, originating from the later fusion experiment, did not share nucleotide homology with any of the examined clones. Data obtained by ELISA, immunosensor, and DNA analysis within a 9 year period are discussed with respect to hybridoma stability, methodic artifacts, measurement reliability, and other possible factors influencing the result interpretation. 相似文献
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