Barbiturate intoxication in two dogs confirmed by toxicological urinalysis

Two dogs were presented within 24 hours to the Department of Small Animal Medicine and Surgery at the University of Veterinary Medicine Hannover for investigation of the sudden onset of neurological abnormalities following a walk in the same park. One dog was observed ingesting a piece of meat. Analysis of urine by gas chromatography-mass spectrometry from each of the dogs identified the presence of barbiturates. Both dogs recovered with supportive treatment. This is the first report to describe the use of toxicological urinalysis with gas chromatography-mass spectrometry for the diagnosis of barbiturate intoxication in dogs.     

An insect growth inhibitor--lufenuron--enhances albendazole activity against hydatid cyst

The aim of this work was to evaluate the potential of lufenuron, a benzylphenylurea with ability to interfere with the formation of insect exoskeleton, as a therapeutic drug for larval echinococcosis (hydatid disease). For this purpose lufenuron, alone or in combination with albendazole, was administered to CD1 mice bearing Echinococcus granulosus hydatid cysts in the peritoneal cavity. Neither of the drugs alone was able to exert parasiticidal effects. However, in combination with albendazole, lufenuron reduced the growth of cysts (30-40% in cyst diameter respect to control, p<0.05). This effect was associated with ultrastructural alterations of the hydatid cyst wall and a reduction of the content of myo-inositol-hexakisphosphate, the major component of the electron dense granules of the laminated layer. Overall, this work provides evidence that lufenuron could represent a useful compound for the use in chemotherapy against larval echinococcosis, by enhancing albendazole parasiticidal activity.     

Aluminum inhibition of NADH‐linked electron transfer by corn root plasma membranes

Vesicles enriched in right‐side‐out plasma membranes were isolated from corn roots by a modified two‐phase partition method. Using reduced nicotinamide adenine dinucleotide as electron donor, isolated vesicles were capable of reducing both ferricyanide and oxygen. At pH 6.0, the presence of Al inhibited both reduction processes to a similar extent. This result indicates that these two reduction processes share at least one common step that is sensitive to Al. Inhibition was not associated with a change in the structure of the membrane domain, as revealed by fluorescence polarization of membrane incorporated probes. These results are the first indications that the electron transfer processes of plasma membranes are sensitive to the presence of Al.     

Potassium inhibition of calcium and magnesium accumulation in roots of intact maize seedlings

The effects of solution potassium (K) on the accumulation of magnesium (Mg) and calcium (Ca) in roots was assessed with 7‐day‐old corn (Zea mays L.) seedlings. Root accumulation of Ca and Mg was studied in replacement type experiments, i.e. the ratio of Ca to Mg was altered but the concentration of Ca plus Mg was kept constant at 2 mM. Concentrations of Ca in the tissue after 12 h exceeded that of Mg, except when Mg comprised over 85% of the divalent cations in the uptake solution. The addition of 0.1 mM K to the Uptake solutions significantly altered the accumulation of Ca and Mg. The concentration of Mg was affected more than that of Ca. The relative effects of solution K on Mg and Ca accumulation in roots could be explained by comparing the relative acid‐base characteristics of the metals.     

Herbage response to spacing of loblolly pine trees in a minimal management silvopasture in southeastern USA

Loblolly pine (Pinus taeda L.) silvopastures often are established and maintained on sites of poor soil fertility and minimal fertilizer input. Our objective was to determine whether row spacing affected yield, quality, and botanical composition of minimally managed herbage in loblolly pine early in the tree rotation. Plots were randomly located equidistant from bordering tree rows in each of eight alley width treatments that were 2.4, 3.6, 4.9, 7.3, 9.7, 12.2, 14.6 m wide, and no trees. Row spacing affected the yield, quality, and botanical composition of pasture five to six years in the rotation especially at densities exceeding 840 trees ha^–1. Botanical composition shifted from predominantly cool-season to warm-season grasses between annual first- and second-harvests, respectively, which caused seasonal differences in several yield and quality traits. Tall fescue (Festuca arundinacea Schreb.) production appeared to be unsustainable under minimal management. Herbage yield generally increased, but quality and minerals (crude protein, IVDMD, Ca, and P) tended to decrease with spacing. The 4.9 m row spacing was minimally acceptable for herbage yield and quality. System design should seek to balance tree-crop yield and quality within the context of management constraints and site productivity.This revised version was published online in November 2005 with corrections to the Cover Date.     

Growth and nut production of black walnut in relation to site, tree type and stand conditions in south-central United States

Black walnut (Juglans nigra) is a prime tree species for agroforestry practices in the United States providing highly prized wood and nuts for human consumption and wildlife. In 54 black walnut stands in south central United States, the site index (i.e., mean dominant height [DH], at age 25 years) ranged between 5.2 and 21.4 m, and was independent of stand density. There were no differences in height and stem diameter (DBH) growth rates between stands with improved varieties and native stock. Most stands were in a “free growth” stage because of either early age or wide spacing. Mean annual increments in DBH and height were positively related both for improved varieties and native stock. Understory competition had a substantial detrimental effect on DH. In a 26 year-old stand, trees growing within Kentucky blue grass (Poa pratensis) had a site index 5 m greater than trees growing within tall fescue (Festuca arundinacea). Predicted nut yields in 2002 varied between 0 and 1370 kg of hulled nuts per ha. Improved varieties had, in general, higher nut yields than predicted from a nut yield-DBH equation developed for individual trees. Nut yields were highly variable both within and among stands, and were related to DBH in native stock but not in improved varieties.     

Medicinal plants used in British Columbia, Canada for reproductive health in pets

In 2003, semi-structured interviews were conducted in British Columbia, Canada with participants obtained using a purposive sample on the ethnoveterinary remedies used for animals. Twenty-nine participants provided the information in this paper on the ethnoveterinary remedies used for reproductive health in dogs and cats. The plants used for pregnancy support and milk production in pets were raspberry-leaf (Rubus idaeus), motherwort (Leonurus cardiaca), flaxseed (Linum usitatissimum) and ginger (Zingiber officinale). Uterine infections were treated with black cohosh (Actaea racemosa) and goldenseal (Hydrastis canadensis). Most of the studies conducted on these plants have not been conducted on companion animals.     

Reproductive performance of sows treated with a combination of pregnant mare's serum gonadotropin and human chorionic gonadotropin at weaning in the summer

During the summer and fall of 1987, sows from eight herds in three states were assigned randomly to receive either a combination of 400 IU of pregnant mare's serum gonadotropin with 200 IU of hCG (P.G. 600) or no treatment at weaning. A treatment x parity interaction was observed for days to first estrus after treatment and percentage anestrus (percentage of sows not achieving estrus within 10 d after weaning). Relative to primiparous control sows, primiparous sows given P.G. 600 expressed estrus sooner (P less than .02) after weaning (6.0 vs 7.8 +/- .6 d) and exhibited less (P less than .02) postweaning anestrus (15.6 vs 29.2 +/- 4.0%). Second parity sows that received P.G. 600 showed estrus sooner (P less than .06) than second-parity control sows (4.7 vs 6.4 +/- .7 d). Days to first estrus after treatment did not differ between groups for parity-three and older sows, and percentage anestrus was not different between treatments for parity-two and older sows. The herd X treatment interaction was significant for percentage recycled (percentage of successfully mated sows that returned to estrus), subsequent farrowing rate, and subsequent number of pigs born dead. Number of pigs born alive was lower for sows treated with P.G. 600 than for control sows (10.55 vs 10.10 +/- .18; P less than .02). In summary, treatment of sows weaned in the summer and fall with P.G. 600 had decreased days to postweaning estrus in parity-one and -two sows and reduced frequency of postweaning anestrus in primiparous sows.