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1.
旨在调查川西北牦牛哺乳动物正呼肠孤病毒(MRV)的感染情况并分离病毒。采用RT-PCR方法,对采自川西北15个牧场的72份牦牛腹泻粪便样本和其中5个牧场的15份腹泻牦牛血清样本进行MRV检测,阳性样本进一步用分型PCR确定其血清型。结果显示,粪便样本中MRV检出率为20.83%(15/72),血清2型的比例为60%(9/15);血清样本中MRV检出率为40%(6/15),血清2型的比例为83.33%(5/6);未检测到其他血清型。成功地从腹泻粪便中分离到1株MRV血清2型毒株(TCID50为4×10-8.56·mL-1),并获得长度为23 587 bp的分离株全基因组,该分离株与中国猪源毒株的遗传关系最近;与GenBank中所有的MRV S1基因相比,该分离株有4个独特的氨基酸突变。本研究从牦牛中检测到MRV,并分离到1株牛源MRV血清2型毒株,为进一步研究MRV血清2型生物学特性奠定了基础。 相似文献
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Still Only One Earth. Progress in the 40 years since the First UN Conference on the Environment. By R.E. Hester & R.M. Harrison (eds). Published by Royal Society of Chemistry,Cambridge, 2015. vii + 285 pp. £67.50. Hardback (ISBN 978‐1‐78262‐076‐1).
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R. L. Hough 《Soil Use and Management》2017,33(1):176-176
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T. Pudert A. Weyrich F. Geburek C. Herden M. Röcken 《Equine Veterinary Education》2021,33(11):e386-e392
Thymoma is a rare condition in horses. This case of a 24-year-old Warmblood gelding that was presented with oedema and signs of vascular congestion of the left forelimb caused by a thoracic mass is described. The diagnostic work-up included blood chemistry, cytology of pleural effusion, ultrasonography, radiography and the visualisation and collection of a tissue sample by thoracoscopy. Finally, post-mortem findings and histopathology revealed thymic epithelial neoplasia with histomorphologic features previously unreported in horses. The unique mixture of spindle-shaped and epithelioid tumour cells in combination with clear features of malignancy suggested the application of the Moran and Suster histological classification system for thymomas in humans (Moran and Suster, 2008, Curr. Treat. Options Oncol., 9, 288), which has not been reported in an equine case before and fits well to the present case. 相似文献
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Jie Kuai Xiaoyong Li Zhen Li Yan Xie Bo Wang Guangsheng Zhou 《Journal of Agronomy and Crop Science》2020,206(6):823-836
Waterlogging is a main factor causing rapeseed (Brassica napus L.) yield loss, and reasonable nitrogen (N) applications can compensate for this loss. To investigate the effects of N rates on seed yield of waterlogged rapeseed, the waterlogging-tolerant rapeseed variety ZS 9 and sensitive variety GH01 were waterlogged for 0 and 10 days with five leaves at the seedling stage under four N rates (0, 90, 180 and 270 kg/ha). Waterlogging significantly decreased seed yield, while N application can alleviate the yield loss. The yield decrease rate of waterlogged GH01 was greater than that of ZS 9 under the same N rate. During the seedling and bolting stage, the leaf photosynthetic rate (Pn) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activity increased, while activities of adenosine diphosphate glucose pyrophosphorylase (AGPase), sucrose synthase (SuSy) and sucrose phosphate synthase (SPS) decreased with more N under the same watering conditions. Compared to the plants without waterlogging, the leaf Pn and Rubisco activity, starch and sucrose contents of waterlogged rapeseed decreased at the two stages; activities of AGPase, SuSy and SPS of waterlogged rapeseed decreased at the seedling while increased at the bolting stage for both the two varieties. At the flowering stage, the Pn, the activities of Rubisco, AGPase, SuSy, SPS and contents of sucrose, starch increased with more N application for both ZS 9 and GH01. Compared to the plants without waterlogging, the Pn and Rubisco activity for waterlogged plants of the two varieties increased; the waterlogged plants of tolerant variety had higher activities of AGPase, SuSy and SPS, while those of sensitive variety was significantly lower. However, the decreased starch and sucrose content were found in both tolerant and sensitive varieties. The activities of AGPase, SuSy and SPS at flowering were highly positively correlated with yield under the interactive effects of N and waterlogging. These results suggested that the flowering stage is the most important stage that N had the positive regulation on waterlogged rapeseed growth. The carbohydrates translocation from leaves to seeds of the tolerant variety were enhanced after waterlogging, while that of the sensitive variety was still inhibited. This was the main reason for the difference in yield between the two waterlogged varieties. 相似文献
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Do cover crops change the lability of phosphorus in a clayey subtropical soil under different phosphate fertilizers? 下载免费PDF全文
A. P. B. Teles M. Rodrigues W. F. Bejarano Herrera A. Soltangheisi L. R. Sartor P. J. A. Withers P. S. Pavinato 《Soil Use and Management》2017,33(1):34-44
Plants have developed different mechanisms to absorb and solubilize phosphorus (P) in the soil, especially in environments with low P availability. This study evaluated the effects of different winter cover crops on soil P availability in a clayey subtropical (Hapludox) soil receiving soluble P fertilizer and a rock phosphate applied to the summer crop, under no‐tillage. The experiment was carried out over 3 yrs (2009–2011) with five different cover crop species: common vetch, fodder radish, ryegrass, black oat, white clover and fallow as control. The soil was sampled after the third year of cover crop cultivation and analysed for inorganic and organic P forms according to the well‐established Hedley fractionation procedure. Phosphate fertilizers promoted accumulation of both labile and nonlabile P pools in soil in the near surface layer, especially under rock phosphate. Fertilizer applications were not able to change P fractions in deeper layers, emphasizing that the Brazilian clayey soils are a sink of P from fertilizer and its mobility is almost nil. Although the cover crops recycled a great amount of P in tissue, in a short‐term evaluation (3 yrs) they only changed the content of moderately labile P in soil, indicating that long‐term studies are needed for more conclusive results. 相似文献
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Mark D. Rawling Nicola Pontefract Ana Rodiles Ilektra Anagnostara Eric Leclercq Marion Schiavone Mathieu Castex Daniel L. Merrifield 《Journal of the World Aquaculture Society》2019,50(6):1108-1122
Fish were fed a single‐strain yeast fraction (SsYF; 2 g/kg) or a multistrain yeast fraction (MsYF; 0.8 g/kg) for 10 weeks. The results demonstrated significant (p ≤ 0.03) elevations in weight gain, specific growth rate, protein efficiency ratio, and feed conversion ratio in fish fed the yeast fraction‐supplemented diets. In the distal intestine, a significant elevation in microvilli density was observed after 5 and 10 weeks of dietary supplementation with MsYF and SsYF, respectively, compared to control fed fish (p < 0.001). A significant elevation (p = 0.02) in the perimeter ratio was observed in fish fed diets supplemented with the yeast fractions. After 10 weeks of feeding on the experimental diets, Rt‐qPCR demonstrated a significant downregulation (p < 0.05) in the stress response genes, heat‐shock protein 70 (hsp70) and proliferating cell nuclear antigen (pcna), in fish fed diets supplemented with the yeast fractions. Significant (p < 0.05) elevations in interleukin 1‐beta (il1β) and interleukin‐10 (il10) gene expression were observed in fish fed diets supplemented with the MsYF compared to the other dietary groups. These findings suggest that feeding an MsYF specifically at a lower incorporation rate < 1 g/kg, compared to a commercial SsYF at 2 g/kg, is effective in improving the intestinal health status and growth performance of European seabass. 相似文献
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“基础化学”理论和实验教学质量的“高与低”在一定程度上直接关乎高职院校与化学相近专业人才培养的“成与败”。以教学改革为切入点,在“基础化学”教材建设和系列微课的制作及应用2个维度上,探讨提升“基础化学”课程教学质量的具体措施。 相似文献