Skeletal muscle characteristics in young trained and untrained standardbred trotters.

Muscle biopsies were taken from the middle gluteal muscle of 28 Standardbred trotters, 3-4 years of age. The 13 horses in Group T were trained consistently from 18 months of age, whereas the 15 horses in Group UT were not exposed to any systematic training before 3 years of age. Group T horses had a lower percentage of Type IIB fibres (31%) than did Group UT horses (39%). Citrate synthase (CS) activity, representing oxidative capacity, was higher in Group T (72 mmol kg-1 min-1) than in Group UT (47 mmol kg-1 min-1). Biopsies were taken from 4 horses in each group when they were foals and then annually until 3-4 years of age. Results from this study indicate that regular training of Standardbreds from 18 months of age resulted in increased CS activity and a decrease in the percentage of Type IIB fibres. This study shows that training, not growth, is the main factor that induces a high oxidative capacity and a high Type IIA/IIB fibre ratio in muscle of Standardbred trotters.     

Studies on pentagastrin-induced hypocalcaemia in lactating cows.

During recent years it has been evident that a number of gastrointestinal hormones are potent calcitonin secretagogues, and it has been suggested that a gastrointestinal-thyroid C cell system exists as a part of post prandial calcium homeostasis. In the present study the hypocalcaemic effect of pentagastrin, a synthetic peptide with gastrin effects, was studied in lactating cows. Intravenous infusion of pentagastrin caused marked hypocalcaemia and hypophosphataemia in the cows. Thyroidectomy completely abolished the hypocalcaemic and hypophosphataemic effects of the peptide. The results thus suggested that the effects of the peptide were due to release of endogenous calcitonin.     

The Effect of Experimental Infectious Mastitis on Leukocyte Subpopulations and Cytokine Production in Non-lactating Ewes

The interactions between leukocytes and cytokines during the acute response to intramammary infections in the dry mammary gland of sheep were studied. Dry ewes were experimentally infected in one udder half with either Staphylococcus aureus or Escherichta coli, or infused with saline as control. Udder secretion samples, blood samples and udder tissue samples were collected before and 4, 8 and 24 h after infections/infusions. Total and differential leukocyte counts were calculated in both blood and mammary secretions, and flow cytometry was used to detect the presence of CD4+, CD8+, WC1+, IL-2R+, CD18+ or L-selectin+ lymphocytes, CD18+ or L-selectin + neutrophils, and CD14+ leukocytes. Moreover, the concentrations of interleukin-1β+ (IL-1β), interleukin-8 (IL-8) and granulocyte-macrophage colony stimulating factor (GM-CSF) in udder secretions were measured using ELISA, and RT-PCR was used to detect the presence of corresponding cytokine mRNA in udder tissue biopsies. The results suggest an association between the concentrations of IL-1β, IL-8 and the intensity of neutrophil infiltration of the infected gland. Immunologically relevant changes in proportions of lymphocyte subpopulations might also occur in the acute phase of the inflammatory reaction of the udder. Greater cellular and cytokine responses to E. coli infection may have contributed to the milder clinical picture and more rapid resolution of infection than that seen for S. aureus. Enhancing the production of pro-inflammatory cytokines may improve defence against bacterial mastitis.     

The effects of dietary NaCl supplement on hypo-osmoregulatory ability and sea water performance of Arctic charr (Salvelinus alpinus L.) smolts

Groups of one‐year‐old smolts of Arctic charr (Salvelinus alpinus L.) reared under a simulated natural photoperiod were fed pelleted feed with a NaCl content of either 1.5% or 9.5% for 6 weeks before release in a river in northern Norway. There were no differences in growth before release between fish fed the two diets. Smolts fed the 9.5% NaCl diet had better hypo‐osmoregulatory ability than those fed the 1.5% diet, and a level of gill Na⁺‐K⁺‐ATPase activity that was several times higher. One of the two groups that had been fed the 9.5% NaCl diet had both a significantly higher recapture rate and growth in sea than the two groups fed the 1.5% NaCl diet, whereas this was not true for the other 9.5% NaCl diet group. The results indicate that a NaCl‐enriched diet could be used to ensure sufficient hypo‐osmoregulatory ability of charr smolts that would otherwise have insufficient regulatory ability.     

Modelling carbon dynamics in mixed grass–red clover swards

Growth and carbon dynamics in mixed grass–red clover leys were simulated using a growth model for pure stands based on radiation use, allocation within plant and loss of biomass. The model and its parameter values were taken from previous applications of the model to pure swards of grass and red clover grown in the same experiment at the same sites and years.     

Micro-scaled high-throughput digestion of plant tissue samples for multi-elemental analysis

Background  

Quantitative multi-elemental analysis by inductively coupled plasma (ICP) spectrometry depends on a complete digestion of solid samples. However, fast and thorough sample digestion is a challenging analytical task which constitutes a bottleneck in modern multi-elemental analysis. Additional obstacles may be that sample quantities are limited and elemental concentrations low. In such cases, digestion in small volumes with minimum dilution and contamination is required in order to obtain high accuracy data.     

Cytokines in mammary lymph and milk during endotoxin-induced bovine mastitis

Cytokine kinetics were examined in milk and in afferent and efferent lymph of the supramammary lymph node after intramammary infusion of endotoxin from Escherichia coli. Cows were sampled 0, 2 and 4h after infusion (p.i.). Neutrophils appeared in afferent lymph 2h p.i., and in efferent lymph and milk 4h p.i. The milk contained high concentrations of interleukin-8 (IL-8) at 2 and 4h p.i. IL-8 was also found in lymph, but at lower concentrations. The tumor necrosis factor-alpha (TNF-alpha) concentration tended to increase in afferent lymph at 2h p.i., and increased in milk at 4h p.i. The level of IL-1beta increased at 4h p.i. in milk, but was not detected in lymph. Interferon-gamma was not detected in any sample, at any time. The results indicate a primary role for IL-8 in the recruitment of neutrophils into the gland, and suggest that IL-1beta and TNF-alpha are not necessary for IL-8 production and release in response to endotoxin.     

Comparison between the Sperm Morphology in Semen Samples Obtained from Yearling Beef Bulls by Transrectal Massage of the Ampullae and Cauda Epididymal Dissection

As electroejaculation (EEJ) is prohibited for use on unanaesthetized animals in Sweden, there is a need for an alternative method of semen collection from bulls in the field. The aim of the present study was to evaluate the use of transrectal massage (TM) of the ampullae to collect semen from yearling beef bulls under field conditions in Sweden. Transrectal massage was performed on 52 yearling beef bulls. Volume of semen collected, duration of procedure, percentage progressively motile sperm, and sperm concentration were measured. Smears were prepared for sperm morphology examination. Semen samples were obtained from 47 of 52 bulls. Mean volume was 3.2 ml (SD +/- 3.7), mean duration of collection was 7.4 min (SD +/- 2.8), mean percentage progressively motile sperm was 43.5% (SD +/- 29.2) and mean concentration was 201.9 x 10(6) spermatozoa/ml (SD +/- 278 x 10(6)). Twenty-three of the 52 bulls were slaughtered 3-4 days after semen collection and aliquots of the cauda epididymal contents were collected for sperm morphology examination. The percentages of proximal droplets, abnormal tails and abnormal midpieces were significantly higher (p < 0.05) and the percentage of detached heads was significantly lower (p < 0.05) in the post-mortem samples compared with those in the TM samples. However, importantly there was no significant difference between the two sample types in the percentages of abnormal heads. This study demonstrates that semen can be collected from yearling beef bulls by TM. We think that TM constitutes a useful tool, when semen collection with EEJ or artificial vagina (AV) is not possible under field conditions, when included in the bull breeding soundness evaluation (BBSE) protocol. However, further studies are needed, and presently being carried out, to evaluate if semen samples collected by TM are comparable with semen samples collected by AV.     

The hypocalcemic response to protamine as a measure of bone resorption

The heparin antagonist protamine inhibits bone resorption. In the present study protamine was given i.v. at a dose of 10 mg/kg to goats and sheep in various stages of pregnancy and lactation. It is known that bone resorption increases as pregnancy progresses. In sheep maximum in bone resportion is reached at early lactation. The hypocalcemic response to protamine followed a similar course. The slightest decrease in serum calcium was obtained during early pregnancy and the greatest during late pregnancy and early lactation. Protamine increased serum inorganic phosphorus. The increase was most pronounced during early pregnancy. Protamine did not significantly change serum magnesium.Five lactating ewes were given 25000 i.u./kg of vitamin D3. Serum calcium increased and the hypocalcemic response to protamine was greater after vitamin D₃ administration than before.The results showed that the hypocalcemic response to protamine can be used as an indicator of the role of bone resorption in calcium homeostasis in various physiological situations.