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Secretory patterns and metabolic clearance rate of porcine growth hormone in swine selected for growth 总被引:2,自引:0,他引:2
J R Arbona D N Marple R W Russell C H Rahe D R Mulvaney J L Sartin 《Journal of animal science》1988,66(12):3068-3072
Eleven Landrace pigs (six boars and five gilts, 50 kg) representing lines selected for three generations for maximum weight at 200 d of age were compared to eight pigs (four boars and four gilts, 50 kg) representing contemporary randomly selected Landrace controls to determine the effect of selection for growth on the metabolic clearance rate (MCR) and plasma concentrations of porcine growth hormone (GH). To estimate MCR of GH, the disappearance of a bolus of porcine GH was monitored over 120 min following its i.v. injection. Blood samples also were collected every 15 min over a 6-h period before injecting GH to determine baseline and overall mean GH concentrations, mean peak amplitude and number of GH secretory episodes. Boars exhibited greater overall mean GH concentrations (4.80 vs 3.11 ng/ml; P less than .05) and had greater maximum GH concentrations associated with secretory episodes (16.11 vs 10.80 ng/ml; P less than .05) than did gilts. There were no differences between boars and pigs exhibited greater baseline GH concentrations (2.04 vs 1.25 ng/ml; P less than .01) than did those from the unselected Landrace line. Selected and control pigs exhibited similar (P greater than .15) overall mean concentrations of GH, frequency of secretory episodes, amplitude of GH peaks and MCR. These data demonstrate that pigs selected for heavier weight at 200 d of age had greater basal plasma GH concentrations than did unselected control pigs. 相似文献
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Simultaneous determination of multiple phytohormones in plant extracts by liquid chromatography-electrospray tandem mass spectrometry 总被引:1,自引:0,他引:1
Durgbanshi A Arbona V Pozo O Miersch O Sancho JV Gómez-Cadenas A 《Journal of agricultural and food chemistry》2005,53(22):8437-8442
A rapid multiresidue method to quantify three different classes of plant hormones has been developed. The reduced concentrations of these metabolites in real samples with complex matrixes require sensitive techniques for their quantification in small amounts of plant tissue. The method described combines high-performance liquid chromatography with electrospray ionization tandem mass spectrometry. Deuterium-labeled standards were added prior to sample extraction to achieve an accurate quantification of abscisic acid, indole-3-acetic acid, and jasmonic acid in a single run. A simple method of extraction and purification involving only centrifugation, a partition against diethyl ether, and filtration was developed and the analytical method validated in four different plant tissues, citrus leaves, papaya roots, barley seedlings, and barley immature embryos. This method represents a clear advantage because it extensively reduces sample preparation and total time for routine analysis of phytohormones in real plant samples. 相似文献
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Pérez-Hedo Meritxell Alonso-Valiente Miquel Vacas Sandra Gallego Carolina Pons Clara Arbona Vicent Rambla José L. Navarro-Llopis Vicente Granell Antonio Urbaneja Alberto 《Journal of pest science》2021,94(4):1221-1235
Journal of Pest Science - Modern agricultural policies across the globe are committed to a significant reduction in chemical pesticide dependency; however, pest management strategies are still... 相似文献
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