排序方式: 共有2条查询结果,搜索用时 0 毫秒
1
1.
H. Rajaei Shoorcheh B. Kazemi S. Manzari J. K. Brown A. Sarafrazi 《Journal of pest science》2008,81(4):199-206
Despite a large number of investigations on the molecular genetics and population structure of the whitefly Bemisia tabaci (Gennadius) complex, no such study had been conducted in Iran. The genetic variation of B. tabaci was examined using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) for 18 field collections
from cucumber, eggplant, and tomato in four provinces of Iran. PCR amplification and restriction digestion with two enzymes
detected 388 RFLP fragments, of which 16 fragments showed polymorphisms. Cluster analysis of these data placed all B. tabaci individuals within a single group, and there was no evidence for between- or within-population genetic variation. Phylogenetic
(Clustal W) analysis of 42 B. tabaci mtCOI sequences (n = 21 field collections) from Iran, and a comparison with well-studied haplotype or biotype reference sequences available
in public sequence databases, revealed that the Iranian B. tabaci populations were most closely related to the B biotype at 0–1.2% nucleotide identity. The B biotype is a well-known member
of a sister clade from the Middle East–North African region of the world, owing to its nearly worldwide distribution and invasive
characteristics. This report indicates that a single major haplotype of B biotype is prevalent in Iran and that its closest
relative is the B biotype. Also, given the extent of known variation in the Middle East and African continent, data indicate
somewhat surprisingly that the B. tabaci collections sampled in Iran had limited genetic variation and population substructure. Knowledge that the B biotype of B. tabaci predominates in Iran is important for designing effective pest management strategies given that biotypes of B. tabaci are known to differ greatly with respect to insecticide resistance, host range, virus–vector interactions, and other key
biological characteristics. 相似文献
2.
Mahnaz Shabani Coralie Bertheau Mehrshad Zeinalabedini Alimorad Sarafrazi Mohsen Mardi Sepehr Mohajeri Naraghi Heshmatollah Rahimian Mahmoud Shojaee 《Journal of pest science》2013,86(2):173-183
Witches’ broom disease of lime, caused by ‘Candidatus Phytoplasma aurantifolia’, is responsible for major losses of Mexican lime trees in Southern Iran, Oman and the United Arab Emirates. The causative phytoplasma is transmitted by the leafhopper, Hishimonus phycitis. We combined ecological niche modelling with environmental and genetic data for six populations of H. phycitis from Iran and one from Oman. The mitochondrial cytochrome c oxidase I (COI) gene and nine microsatellite DNA markers were used for the genetic analyses. Although the Oman population had specific haplotypes, the COI sequences were highly conserved among all populations studied. In contrast, the microsatellite data divided the populations from Iran and Oman into two separate clades. An analysis of molecular variance indicated a high level of variation within populations. The Mantel test showed no correlation between genetic and geographical distances. Gene flow values were small between the populations from Iran and north of Oman but significantly higher among the Iranian populations supporting the differentiation between Iran and Oman. In addition, we found that patterns of genetic divergence within Iranian populations were associated strongly with divergence in terms of their ecological niches. Data on six climatic variables, including elevation, were used to create ecological niche models. Our results suggest that the genetic differentiation of H. phycitis may be attributable to climatic conditions and/or geographical barriers. 相似文献
1