新型纳米PE膜对温室环境因子的影响（英文）

[目的]检测新型纳米流滴控释膜的流滴性能及其对温室环境因子的影响。[方法]通过设施叶菜类农业大棚智能监控系统,对流滴控释膜和对照膜内的空气温度、光照强度和CO2浓度等进行检测和分析。[结果]通过大田扣棚跟踪监测数据发现,这种膜具有比现有长寿、流滴功能膜光照强度高、减少高温天气棚温上升以及具有更好的光合作用效果等优点。[结论]这种新型的纳米流滴控释膜比现在广泛使用的国产流滴膜性能更优越,应用前景广泛。     

Analysis of Spectral Characteristics Based on Optical Remote Sensing and SAR Image Fusion

Because of cloudy and rainy weather in south China, optical remote sens-ing images often can＇t be obtained easily. With the regional trial results in Baoying, Jiangsu province, this paper explored the fusion model and effect of ENVISAT/SAR and HJ-1A satel ite multispectral remote sensing images. Based on the ARSIS strat-egy, using the wavelet transform and the Interaction between the Band Structure Model （IBSM）, the research progressed the ENVISAT satel ite SAR and the HJ-1A satel ite CCD images wavelet decomposition, and low/high frequency coefficient re-construction, and obtained the fusion images through the inverse wavelet transform. In the light of low and high-frequency images have different characteristics in differ-ent areas, different fusion rules which can enhance the integration process of self-adaptive were taken, with comparisons with the PCA transformation, IHS transfor-mation and other traditional methods by subjective and the corresponding quantita-tive evaluation. Furthermore, the research extracted the bands and NDVI values around the fusion with GPS samples, analyzed and explained the fusion effect. The results showed that the spectral distortion of wavelet fusion, IHS transform, PCA transform images was 0.101 6, 0.326 1 and 1.277 2, respectively and entropy was 14.701 5, 11.899 3 and 13.229 3, respectively, the wavelet fusion is the highest. The method of wavelet maintained good spectral capability, and visual effects while improved the spatial resolution, the information interpretation effect was much better than other two methods.     

硝化还原假单胞菌SPQ03 PnSoxRS调控子的克隆和功能初步分析

从对百草枯具有高度抗性的硝化还原假单胞菌(Pseudomonas nitroreducens)菌株SPQ03中分离了PnSoxR和PnSoxS调控子.ScanProsite程序分析结果表明,SoxR氨基端含有一个MerR家族特有的DNA结合域,羧基端含有4个半胱氨酸残基,是MerR家族成员之一;SoxS是AraC家族成员,含有2个典型的AraC-HTH保守域;PnSoxR所编码的氨基酸与大肠杆菌SoxR的一致性为98%,而与同属的铜绿假单胞菌(Pseudomonas aeruginos)SoxR的一致性仅为56%,PnSoxR与大肠杆菌SoxS的一致性为100%,铜绿假单胞菌中没有发现SoxR基因存在.将PnSoxR和PnSoxS分别转化E.coli BL21,发现PnSoxS能赋予BL21百草枯(Paraquat)抗性.     

布鲁氏杆菌LPS抗原制备及其免疫原性测定

采用热酚水法提取布鲁氏杆菌脂多糖,分离、纯化并SDS-PAGE银染鉴定。纯化的细菌内毒素免疫小鼠,通过斑点ELISA测定血清抗体效价,检测细菌内毒素免疫原性。采用热酚法能获得较高纯度的细菌内毒素,其免疫原性较高,斑点ELISA测血清效价达到1∶1 000 000,与全菌免疫原性接近。本研究为制备布鲁氏杆菌单克隆抗体和建立布鲁氏杆菌病快速诊断方法奠定研究基础。     

MeJA regulates enzymes involved in ascorbic acid and glutathione metabolism and improves chilling tolerance in loquat fruit

This study investigated the regulation of ascorbate acid (AsA) and glutathione metabolism and chilling tolerance by methyl jamonate (MeJA) in loquat fruit. The results showed that application of MeJA to loquat fruit inhibited the incidence of chilling injury manifested as internal browning (IB) and increased AsA and reduced glutathione (GSH) contents due to the inhibition of ascorbate oxidase activity and enhancement of monodehydroascorbate reductase, dehydroascorbate reductase and glutathione reductase activities. Meanwhile, MeJA also enhanced activities of ascorbate peroxidase, glutathione peroxidase and glutathione-S-transferase. Our results suggested that MeJA can regulate the ascorbate and glutathione metabolism and has important roles in alleviating oxidative damage and enhancing chilling tolerance in loquat fruit.     

长江下游地区不同水稻品种两种行距下的 生长发育和产量及品质特征

为研究种植行距对水稻生长发育和产量及品质的影响,选取15个不同类型水稻品种为试验材料进行不同行距种植处理,设置两种行距分别为30 cm和25 cm,株距均为13.3 cm,两种行距栽培密度分别为251 000 和301 000穴·hm^-2,对水稻生长发育情况和产量及品质性状进行分析。结果显示：水稻品种间生长发育情况和产量及品质各指标间呈显著或极显著差异,行距30 cm处理可以显著增加水稻的株高、剑叶长和剑叶宽,提高水稻的结实率、每穗粒数,有利于产量的形成;行距25 cm处理单位面积内的有效穗数明显增大。丰粳3227、镇稻14、苏垦118、嘉优1号、嘉优3号和甬优2640等品种产量较高,经济性状突出,建议采用25 cm行距进行种植,甬优2640采用30 cm行距效果更好,南粳9108采用25 cm与30 cm均可。该研究为确定适宜栽培行距并筛选得出适宜泰州地区种植的优异水稻品种资源提供了一定的科学依据。