This article aims to establish an efficient assay for screening monoclonal antibodies (McAbs) against the membrane proteins of chicken embryo fibroblast (CEF) for further studies of the cellular receptors of infectious bursal disease virus (IBDV). McAbs against the membrane proteins of CEF were prepared by cell fusion. The monolayer CEF pre-incubated with the CEF-specific McAbs for 2 h were infected with IBDV and incubated with F22-EA6-biotin postinfection. Then, the cells were reacted with streptavidin-horseradish peroxidase (HRP) and finally stained by 3-amino-9-ethylcarbazole (AEC). The inhibitive percentage of IBDV infection was calculated by counting the IBDV-infected cells to determine the inhibition efficiency of the CEF-specific McAbs. Compared with the control cells, the IBDV-infected cells pretreated with CEF-specific antibody significantly decreased; supernatant fluids of a total of 768 hybridomas were analyzed. The results of immunohistochemistry assays showed that six of them (1A5, 1H11, 2B 12, 3G1, 4D10, and 4B8) have the abilities to block the infection of IBDV to CEF, among which 4B8 can perfectly block the infection. This novel method is a sensitive and specific assay for the screening of CEF membrane protein-specific McAbs, which can block the infection of IBDV to CEF, and these McAbs can be used for the further investigations of the cellular receptors of IBDV. 相似文献
In arid and populated areas or countries, water shortage and heavy carbon emissions are threatening agricultural sustainability with food security severely, and becoming a major issue. It is unclear whether improved farming systems can be developed to tackle those issues through a sustainable agriculture. Here three farming practices that have proven to be essential and successful, which were: (a) crop intensification through strip intercropping, (b) water harvesting through conservation tillage; and (c) carbon sequestration through improved crop residue management options, were integrated in one cropping system. We hypothesize that the integrated system allows the increase of crop yields with improved water use efficiency, while reducing carbon emissions from farming. The hypothesis was tested in field experiments at Hexi Corridor (37°96′N, 102°64′E) in northwest China. We found that the integrated system increased soil moisture (mm) by 7.4% before sowing, 10.3% during the wheat–maize co-growth period, 8.3% after wheat harvest, and 9.2% after maize harvest, compared to the conventional sole cropping systems. The wheat/maize intercrops increased net primary production by 68% and net ecosystem production by 72%; and when combined with straw mulching on the soil surface, it decreased carbon emissions by 16%, compared to the monoculture maize without mulch. The wheat/maize intercrops used more water but increased grain yields by 142% over the monoculture wheat and by 23% over the monoculture maize, thus, enhancing water use efficiency by an average of 26%. We conclude that integrating strip intercropping, conservation tillage as well as straw mulching in one cropping system can significantly boost crop yields, improve the use efficiency of the limited water resources in arid areas, while, lowering the carbon emissions from farming. The integrated system may be considered in the development of strategies for alleviating food security issues currently experienced in the environment-damaged and water-shortage areas. 相似文献