全文获取类型
| 收费全文 | 1050篇 |
| 免费 | 357篇 |
| 国内免费 | 41篇 |
专业分类
| 林业 | 53篇 |
| 农学 | 71篇 |
| 基础科学 | 1篇 |
| 39篇 | |
| 综合类 | 431篇 |
| 农作物 | 59篇 |
| 水产渔业 | 130篇 |
| 畜牧兽医 | 401篇 |
| 园艺 | 45篇 |
| 植物保护 | 218篇 |
出版年
| 2025年 | 40篇 |
| 2024年 | 38篇 |
| 2023年 | 56篇 |
| 2022年 | 90篇 |
| 2021年 | 92篇 |
| 2020年 | 94篇 |
| 2019年 | 79篇 |
| 2018年 | 73篇 |
| 2017年 | 68篇 |
| 2016年 | 75篇 |
| 2015年 | 67篇 |
| 2014年 | 68篇 |
| 2013年 | 67篇 |
| 2012年 | 78篇 |
| 2011年 | 86篇 |
| 2010年 | 77篇 |
| 2009年 | 57篇 |
| 2008年 | 52篇 |
| 2007年 | 44篇 |
| 2006年 | 27篇 |
| 2005年 | 33篇 |
| 2004年 | 17篇 |
| 2003年 | 18篇 |
| 2002年 | 13篇 |
| 2001年 | 5篇 |
| 2000年 | 11篇 |
| 1999年 | 4篇 |
| 1998年 | 2篇 |
| 1997年 | 2篇 |
| 1996年 | 1篇 |
| 1995年 | 1篇 |
| 1994年 | 2篇 |
| 1993年 | 1篇 |
| 1991年 | 1篇 |
| 1990年 | 2篇 |
| 1956年 | 2篇 |
| 1955年 | 5篇 |
排序方式： 共有1448条查询结果，搜索用时 9 毫秒
1.
ABSTRACT: Paralytic shellfish poisoning (PSP) toxins produced by Alexandrium isolates from Korea were analyzed by high-pressure liquid chromography. Species designation of the regional isolates was determined by morphological criteria and ribotyping inferred from sequences of the 28S rDNA D1-D2 region. Toxin analysis performed at the exponential growth phase, revealed that the two strains of A. fraterculus were non-toxic, while the strains of A. tamarense and A. catenella were toxic. Toxic isolates DPC7 and DPC8 of A. catenella produced GTX1, 2, 3, 4, 5, dcGTX2, 3, C1, 2, neoSTX and STX with trace or non-detectable levels of C3 and C4, while isolates UL7, KDW981, SJW97043, SJW97046, KJC97111 and KJC97112 of A. tamarense produced GTX1, 2, 3, 4, dcGTX3, C1, 2, neoSTX with trace or non-detectable levels of C3, 4, dcSTX and STX, and no GTX5 and dcGTX2. The major toxins produced by A. catenella were C1 +2, and those of A. tamarense were C1 +2 and GTX4 in most of the isolates. A. tamarense strains other than SJW97046 produced a relatively high proportion of carbamate toxins, reflecting the high toxicity scores of shellfish intoxication in sampled coastal areas. Two representative toxic isolates, A. tamarense SJW97043 and A. catenella DPC7, were cultured for 30 days in batch mode and subjected to toxin analysis at 5-day intervals. Comparison of toxin productivity in terms of total toxin content, toxin components, and their variations with culture age revealed marked differences between the two strains. 相似文献
2.
3.
Randomly amplified polymorphic DNA (RAPD) markers were used for the identification of two pigeonpea cytoplasmic genic male sterile (CMS) lines derived from crosses between the wild (Cajanus scarabaeoides & C. sericeus) and the cultivated species of Cajanus cajan. The male sterile (A) line and its maintainer (B) line could be easily differentiated with certain random primers. The two male sterile (288 A and 67 A)systems are based on C. scarabaeoidesand the other is based on C. sericeus could also be differentiated. Amplification product of 600bp amplified by primer OPC-11 was observed in both the cytoplasmic male sterile lines (288 A and 67A), which was absent in the maintainer lines (288 B and 67 B) and the putative R-line (TRR 5 and TRR 6). CMS lines, putative R lines, other cultivars and wild species under the study could be easily distinguished with the help of different primers. Dendrogram constructed based on the similarity index showed that considerable genetic variation exist sbetween CMS lines, two putative R line and wild species studied. This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
4.
Mitsuyuki Tomiyoshi Yasuo Yasui Takanori Ohsako Cheng-Yun Li Ohmi Ohnishi 《Breeding Science》2012,62(3):241-247
Fagopyrum homotropicum Ohnishi is a self-pollinating wild buckwheat species indigenous to eastern Tibet and the Yunnan and Sichuan Provinces of China. It is useful breeding material for shifting cultivated buckwheat (F. esculentum ssp. esculentum Moench) from out-crossing to self-pollinating. Despite its importance as a genetic resource in buckwheat breeding, the genetic variation of F. homotropicum is poorly understood. In this study, we investigated the genetic variation and phylogenetic relationships of the diploid and tetraploid forms of F. homotropicum based on the nucleotide sequences of a nuclear gene, AGAMOUS (AG). Neighbor-joining analysis revealed that representative individuals clustered into three large groups (Group I, II and III). Each group contained diploid and tetraploid forms of F. homotropicum. We identified tetraploid plants that had two diverged AG sequences; one belonging to Group I and the other belonging to Group II, or one belonging to Group II and the other belonging to Group III. These results suggest that the tetraploid form originated from at least two hybridization events between deeply differentiated diploids. The results also imply that the genetic diversity contributed by tetraploidization of differentiated diploids may have allowed the distribution range of F. homotropicum to expand to the northern areas of China. 相似文献
5.
Between 1998 and 2009, the four tomato‐infecting begomovirus species detected in Taiwan were Ageratum yellow vein Hualien virus (AYVHuV), Tomato leaf curl Taiwan virus (ToLCTWV), Tomato yellow leaf curl Thailand virus (TYLCTHV) and a newly defined species Tomato leaf curl Hsinchu virus (ToLCHsV). AYVHuV was detected occasionally in 2003 and ToLCHsV only in 2000–2001, whilst ToLCTWV was detected throughout the period. TYLCTHV was first detected in 2005. Between 1998 and 2005, >99% of the begomovirus‐positive samples were infected with ToLCTWV. In 2007 in western Taiwan, 16% of the positive samples were infected with ToLCTWV, 35% with TYLCTHV and 49% with mixed infection (ToLCTWV/TYLCTHV). In contrast, in eastern Taiwan the proportions were 84% ToLCTWV, 2% TYLCTHV and 14% mixed infection. However, throughout Taiwan in 2008–2009, most positive samples were either identified as TYLCTHV (51%) or mixed infection (ToLCTWV/TYLCTHV; 41%), and only 8% were ToLCTWV. This shows a clear trend of shifting from ToLCTWV to TYLCTHV and mixed infection over a short time period in Taiwan. Sequence analyses indicated that tomato‐infecting AYVHuV, an apparent recombinant between ToLCTWV and AYVHuV from Ageratum, represents a new strain Hsinchu. TYLCTHV Taiwan isolates were highly similar to each other, whereas ToLCTWV isolates had greater diversity and were classified into three strains which had one country‐wide and two local distributions. ToLCTWV and TYLCTHV were confirmed as monopartite and bipartite begomoviruses, respectively, by agroinfection followed by transmission with Bemisia tabaci biotype B. In addition, TYLCTHV was found to be mechanically transmissible together with viral DNA‐B. 相似文献
6.
A. Moreira‐Soto N. Vargas‐Vargas B. Rodríguez‐Herrera C. Jiménez E. Corrales‐Aguilar 《Zoonoses and public health》2015,62(7):501-505
Bats are hosts of diverse coronaviruses (CoVs) known to potentially cross the host–species barrier. For analysing coronavirus diversity in a bat species‐rich country, a total of 421 anal swabs/faecal samples from Costa Rican bats were screened for CoV RNA‐dependent RNA polymerase (RdRp) gene sequences by a pancoronavirus PCR. Six families, 24 genera and 41 species of bats were analysed. The detection rate for CoV was 1%. Individuals (n = 4) from four different species of frugivorous (Artibeus jamaicensis, Carollia perspicillata and Carollia castanea) and nectivorous (Glossophaga soricina) bats were positive for coronavirus‐derived nucleic acids. Analysis of 440 nt. RdRp sequences allocated all Costa Rican bat CoVs to the α‐CoV group. Several CoVs sequences clustered near previously described CoVs from the same species of bat, but were phylogenetically distant from the human CoV sequences identified to date, suggesting no recent spillover events. The Glossophaga soricina CoV sequence is sufficiently dissimilar (26% homology to the closest known bat CoVs) to represent a unique coronavirus not clustering near other CoVs found in the same bat species so far, implying an even higher CoV diversity than previously suspected. 相似文献
7.
Shengtao FAN Tiecheng WANG Xiaolong GAO Ying YING Xue LI Yongcheng LI Yuanguo LI Jinzhu MA Heting SUN Dong CHU Yu XU Songtao YANG Qihan LI Yuwei GAO Xianzhu XIA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1143-1149
Newcastle disease virus (NDV) causes a highly contagious viral disease in poultry andwild birds, and it can cause significant economic loss worldwide. Eight viral strains wereisolated by inoculating embryonated chicken eggs from the Poyang Lake region of China withswab samples. All eight of the NDV isolates were identified as class I genotype 3 strains,but they diverged notablely from class II viruses. Further analysis revealed that alleight NDV isolates were lentogenic strains containing the112ERQER↓L117 motif at the F protein cleavage site. The strainswere highly identical and were more species specific (chicken and waterfowl) than sitespecific (Nanchang and Duchang regions). The close phylogenetic proximity of theseisolates indicates that viral transmission may happen between poultry and wild birds. Ourstudy demonstrates that lentogenic class I NDVs exist in clinically healthy wild waterfowland poultry within the Poyang Lake region. Active surveillance of these viruses todetermine their evolution and origin is one of the most realistic strategies forpreventing and controlling NDV outbreaks. 相似文献
8.
Giulia Bedendo Valentina Panzarin Andrea Fortin Gianpiero Zamperin Tobia Pretto Alessandra Buratin Rosita Quartesan Matteo Sabbion Cristian Salogni Francesco Pascoli Anna Toffan 《Journal of fish diseases》2018,41(7):1063-1075
This study fully describes a severe disease outbreak occurred in 2016 in black bullhead catfish farmed in Italy. Affected fish showed nervous clinical signs as well as emaciations and haemorrhagic petechiae on the skin at the fin bases, abdomen and gills. Viral isolation in cell culture allowed the subsequent identification of a rhabdovirus, tentatively named ictalurid rhabdovirus (IcRV), through electron microscopy, immunofluorescence and whole genome sequencing (WGS). The newly isolated virus, together with 14 additional viral strains stored in our repository and detected during similar mortality episodes in the period 1993–2016, was phylogenetically analysed on the basis of the nucleoprotein and the glycoprotein nucleotide and amino acid sequences. The genetic distances among Italian IcRV strains were also estimated. Our results show that all the IcRV strains belong to the genus Sprivivirus and are closely related to the tench rhabdovirus (TenRV). Italian catfish production is constantly decreasing, mainly due to viral infections, which include the newly characterized IcRV. Data presented in this work will assist to investigate the molecular epidemiology and the diffusive dynamics of this virus and to develop adequate surveillance activities. 相似文献
9.
Eric V VIRTUDAZO Hitoshi NAKAMURA Makoto KAKISHIMA 《Journal of General Plant Pathology》2001,67(1):28-36
Phylogenetic analysis of sugarcane rusts based on sequences of ITS and the 5.8 S rDNA revealed two highly divergent ITS groups
among isolates of Puccinia sp. sensu Muta, 1987 and P. kuehnii specimens. Although there is sufficient divergence (exceeding normal intraspecific variation) between the ITS regions of the
two groups to support separation into different species, unusually high homology of the ITS group I sequences with those of
members of Cronartium and identical sequences of the D1/D2 regions of the LSU rDNA for all the isolates of “Puccinia sp.” and P. kuehnii that otherwise exhibited different ITS sequences, suggest that the two highly divergent sequences may have resulted from abnormal
genetic events leading to non-orthologous, intraspeciflc polymorphisms. The other sugarcane rust, P. melanocephala and the grass rusts, P. miscanthi and P. rufipes, were separated from “Puccinia sp.” and P. kuehnii and from each other in D1/D2 region analyses, indicating that D1/D2 region sequences may more correctly reflect phylogenetic
relationships in these rusts than do the ITS regions. Further studies to examine differences in patho-genicity or finer morphological
features within P. kuehnii that may be correlated with the high divergence in ITS sequences and experiments to determine if these two sequence types
represent intraspeciflc polymorphism are necessary.
Received 11 October 2000/ Accepted in revised form 24 November 2000 相似文献
10.
Shouchuan Jiang Nini Zhou Yufeng Li Jiahui An Tianhao Chang 《Veterinary Medicine and Science》2019,5(2):176-181
Porcine circovirus 3 (PCV3), a recently discovered virus, has spread widely in pigs throughout the world. In order to investigate the possibility of mice used to study the infection of PCV3, commercially sourced Balb/C and ICR mice were screened for PCV3 infection. Blood samples were collected from 20 mice (10 each of Balb/c and ICR), DNA was extracted, and subjected to PCR with PCV3 specific primers. We found all 20 serum samples tested positive for PCV3 DNA. From four mice, the complete genomes of PCV3 were amplified and sequenced, and a phylogenetic tree was constructed. The results showed that the amplified genome was 2000 bp, and sequence comparison showed that the homology of the complete genome and ORF2 gene with those of porcine PCV3 are 97.9%–98.8% and 96.9%–98.3%, respectively. Amino acids alignment results showed that the Cap protein of the mouse PCV3 isolates share 90.7%–96.3% amino acid homology with that of the references strains derived from pigs. Phylogenetic analysis based on ORF2 sequences showed that all PCV3 strains clustered together and were clearly separate from other circovirus species. We detected PCV3 in experimental mice in China for the first time, which is an opportunity to use mice to study the infection of PCV3 and a potential hazard to swine industry. 相似文献