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用32个蚕品种杂交形式供试,比较了新老2种稚蚕人工饲料配方的疏毛率;结果表明新配方的24 h疏毛率普遍优于老配方,差异达到极显著水平;24 h疏毛率,老配方中只有6个蚕品种超过了98%,新配方中有21个蚕品种超过了98%,增加了15个蚕品种型式,新配方明显扩大了一代杂交种杂交率检验和小蚕共育的适用蚕品种范围.从不同龄期小蚕人工饲料育与全龄桑叶育的养蚕成绩和茧丝成绩看,用人工饲料进行小蚕共育基本可行,综合考虑各方面因素,共育到2龄后再改喂桑叶或分蚕出售给农民较好.  相似文献   
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The Mtentu River has a straight open tidal estuary flowing between steep grassy or indigenous forest-covered slopes. Total high-water surface area is about 0,3 km.a The zooplankton was studied by periodic sampling over a period of thirteen months. Some physical characteristics of the estuary are presented. The seasonal and spacial distribution and relative importance of four species of mysid, is discussed. The most common mysid in the estuary was Mesopodopsis africana, although Rhopalophthalmus terrcmatalis, in view of its larger size, contributed more to the overall mass of mysids in the samples.  相似文献   
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为了解重庆市山羊博尔纳病隐性带毒情况,分析博尔纳病病毒(BDV)的种系来源。采用巢式逆转录酶PCR结合荧光定量PCR(FQ—nRT—PCR)技术对重庆市60只山羊外周血单核细胞(PBMCs)及脑组织中的BDVP24基因片段进行了检测,将阳性产物测序,并与国外BDV毒株进行了比较。结果,山羊外周血检测阳性率为8.3%(5/60),脑组织检测阳性率为10%(6/60)。该BDVP24片段核苷酸序列与马源BDVH1766株同源性最高,达96.519/6,与标准株Strain V和He/80同源性为95.35%,并且编码的氨基酸序列也相同。表明,重庆市山羊中存在动物源性博尔纳病隐性带毒,该BDVP24核苷酸序列与Strain V和He/80株具有高度同源性。  相似文献   
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Glasshouse experiments were conducted to evaluate whether herbicide application rates could be reliably reduced without compromising the efficacy of the herbicide. The seedling, and vegetative and preflowering plants of nodding thistle ( Carduus nutans ) and hairy buttercup ( Ranunculus sardous ) were treated with different rates of glyphosate or a mixture of picloram and triclopyr. Half of the plants were well-watered at all times while the other half was moisture-stressed for 1 week before the herbicide treatments were applied. Hairy buttercup was more susceptible to glyphosate than nodding thistle, while both were equally susceptible to the picloram/triclopyr mixture. Moisture stress significantly reduced the efficacy of both herbicide treatments, regardless of the plant development stage or the herbicide rate applied.  相似文献   
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紫茎泽兰是一种重要的外来入侵生物,已在我国西南地区泛滥成灾。为了探明甲嘧磺隆和氨氯吡啶酸对紫茎泽兰光合特性的影响,为紫茎泽兰高效化学防除提供参考依据,采用单因素随机区组设计,对盆栽紫茎泽兰喷施不同浓度的甲嘧磺隆可溶性粉剂和氨氯吡啶酸水剂,测定施药后1~7 d紫茎泽兰几个光合特性参数。结果表明,随浓度和时间的增加,紫茎泽兰的光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、胞间CO2浓度(Ci)及叶绿素含量呈下降趋势。0.12 g.L-1甲嘧磺隆和1.75 g.L-1氨氯吡啶酸能有效地抑制紫茎泽兰的光合作用,这两种药剂用量几乎减少一半。  相似文献   
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【目的】探讨外源EBR(24-表油菜素内酯)对低氧胁迫下黄瓜(Cucumis sativus L.)根系的生长和抗氧化系统的影响。【方法】采用营养液水培法,以抗低氧能力较强的黄瓜品种绿霸春4号和抗低氧能力较弱的品种中农8号为材料,研究根际低氧胁迫下外源施用EBR对黄瓜根系生长、ROS及抗氧化酶活性的影响。【结果】在低氧胁迫下,EBR处理显著促进了低氧胁迫下两品种黄瓜幼苗根系生长,提高了SOD、APX、GR活性及AsA、GSH含量,降低了O 、H2O2及MDA含量。【结论】外源EBR处理通过促进低氧胁迫下黄瓜幼苗根系中抗氧化酶活性和抗氧化剂含量的提高,降低ROS含量,增强植株抗低氧胁迫的能力。  相似文献   
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Virus-induced gene silencing (VIGS) offers a rapid and high throughout technology platform for the analysis of gene function in plants. The barley stripe mosaic virus (BSMV) VIGS system was optimized in studies silencing phytoene desaturase expression in wheat, and demonstrated that infection with BSMV construct carrying a 412 bp fragment of TaRAR1 caused conversion of incompatible to compatible interactions to Lr24-mediated resistance in wheat TcLr24 and cultivar 5R615 harboring Lr24 whereas infection with a control construct had no effect on resistance or susceptibility. RTPCR analysis showed that BSMV-induced gene silencing could be detected at mRNA levels. These studies indicated that TaRAR1 was a required component for Lr24-mediated race-specific resistance and the BSMV-VIGS was a powerful tool for dissecting the genetic pathways of disease resistance in hexaploid wheat. Abstract Virus-induced gene silencing (VIGS) offers a rapid and high throughout technology platform for the analysis of gene function in plants. The barley stripe mosaic virus (BSMV) VIGS system was optimized in studies silencing phytoene desaturase expression in wheat, and demonstrated that infection with BSMV construct carrying a 412 bp fragment of TaRAR1 caused conversion of incompatible to compatible interactions to Lr24-mediated resistance in wheat TcLr24 and cultivar 5R615 harboring Lr24 whereas infection with a control construct had no effect on resistance or susceptibility. RTPCR analysis showed that BSMV-induced gene silencing could be detected at mRNA levels. These studies indicated that TaRAR1 was a required component for Lr24-mediated race-specific resistance and the BSMV-VIGS was a powerful tool for dissecting the genetic pathways of disease resistance in hexaploid wheat.
相似文献   
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A likely inherited disease with gross and microscopic features of rickets has been recognised in Corriedale sheep in New Zealand, and a defect in end-organ responsiveness to vitamin D has been proposed as a likely mechanism. The aim of the present study was to characterize the mode of inheritance and determine the disease mechanism. Breeding trials showed that the mode of inheritance was autosomal recessive. Serum chemistry testing using different methodology and studies in cultured skin fibroblasts did not support our previous hypothesis of a defect in end-organ responsiveness. The studies revealed normal serum 1,25-dihydroxyvitamin D concentrations, normal vitamin D receptor function, and the presence of 24-hydroxylase mRNA in cells from affected sheep, even without induction by 1,25-dihydroxyvitamin D3. In addition, osteocalcin mRNA expression was similar in both affected and control sheep. It was concluded that increased expression of 25-hydroxyvitamin D3-24-hydroxylase, the enzyme that breaks down vitamin D, may be involved in the pathogenesis of inherited rickets in Corriedale sheep, but its role requires further clarification.  相似文献   
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