松材线虫萎蔫病生物学特性及治理技术的初步研究

松材线虫萎蔫病病原是松材线虫(Bursaphelenchus xylophilus),而松褐天牛(Monochamus alternatus)是主要传病媒介。根据南京地区自然规律、线虫和天牛的生物学特性,采用了树干注药、薰蒸杀灭病死木中的传病媒介、喷洒菊酯微胶囊农药、加强营林技术管理和制定严格的植物检疫制度等综合防治方法,能有效地防治松材线虫萎蔫病。     

Extraction of soil nitrogen by chloroform fumigation – A new index for the evaluation of soil nitrogen supply

The N extracted after chloroform (CHCl₃) fumigation was determined as a possible index of soil N supply to plants. The relationships between extractable N following fumigation and reference indices such as total N, alkali-hydrolyzable N, N released by the Stanford short-term incubation method, and the N extracted by KCl and by CaCl₂, were measured in nine soils of differing soil N supply capacity. A highly significant correlation was achieved between the extractable N released by fumigation and the N released by the Stanford method, i.e. a short-term aerobic incubation (r = 0.87). Similarly, the correlation between extractable N by fumigation and the N uptake by ryegrass was highly statistically significant (r = 0.93). Using the N extracted following fumigation has the advantage that laboratory results are available in two days and are both reproducible and of high precision. Therefore, the N extracted following fumigation is a valid, timesaving and precise index of soil N supply capacity.     

甲酸乙酯的施药高度对仓内空间杀虫效果的影响

在储粮容量7000t的高大平房仓空仓内,设置一个高7.50 m、直径300mm的熏蒸室,分别在7.50m、5.61m、3.74m、1.87m和0.1m的高度放置试虫,先后从0.10m、3.74m和7.50m的高度施药,测定了甲酸乙酯对处于不同高度的赤拟谷盗(Tribolium castaneum(Herbst))和谷蠹(Rhyzopertha dominica(Fabricius))成虫的杀虫效果。结果为:在0.10m高度施药,甲酸乙酯对于靠近施药点的试虫可完全致死,而在1.87m及其以上高度处的害虫死亡率均显著低于施药点的死亡率。在高度3.74m处施药,施药点及其以下两种害虫的死亡率均显著大于施药点以上的害虫死亡率,3.74m及其以下的谷蠹均完全死亡,赤拟谷盗的死亡率则出现了0.1m处100%,1.87m和3.74m处则分别只有96.7%和81%。经7.50m高度施药,0.10m高度处的害虫死亡率达100%,且害虫死亡率出现自下而上递减的结果。本实验表明,作为熏蒸剂的甲酸乙酯在空间被施用后,静态条件下向上扩散且有效杀虫的高度小于2m;在高空施药后主要扩散趋势为向下运动(下沉),对施药点以下的害虫效果较好,建...     

活塞式挤奶机节拍比与真空时相比测试研究

挤奶机的节拍比、最大和最小真空时相比是影响挤奶机正常工作、挤奶速度、乳牛健康等的因素，该文利用电测技术，分别在空载和带载两种状态下，测试和分析研究了活塞式挤奶机的节拍比、最大真空时相比和最小真空时相比，通过测试分析表明，所研究活塞式挤奶机的节拍比、最小真空时相比、最大真空时相比均不完全满足标准规定值。     

嗜酸乳杆菌真空喷雾干燥微胶囊化技术的研究

以阿拉伯胶、β-环糊精、可溶性淀粉、明胶、脱脂奶粉为壁材，根据正交试验确定微生态菌株嗜酸乳杆菌细胞微胶囊化的最佳真空喷雾干燥进风温度、壁材浓度和芯材浓度，并对其粒子直径进行分析。结果表明，β-环糊精与阿拉伯胶以9∶1配比为理想壁材，喷雾干燥进风温度170℃、壁材浓度20%和芯材浓度6%为最佳组合，活菌数达10⁸ cfu/g以上，微胶囊粒子直径平均为20 μm。     

混合动力汽车下坡辅助电-液复合制动控制方法

为提高混合动力汽车下坡辅助控制中电、液复合制动的综合性能,该文提出一种综合考虑整车安全及经济性的电、液复合制动控制方法。通过对混合动力汽车下坡辅助制动转矩变化过程及各辅助制动系统特性的分析,拟定了以安全性为基础、以经济性为目标的下坡辅助制动转矩分配原则,利用前馈加反馈的控制方法制定了电机辅助制动系统及液压辅助制动系统的转矩协调控制策略。并通过仿真及试验平台对以上算法进行了验证,结果表明该方法可以有效地减小液压系统启动延时,保证了液压辅助制动系统的响应速度及下坡辅助系统整体的响应精度。该研究提高了整车控制的安全性和经济性,也为电动车辆复合制动进一步研究提供了思路。     

Characterization of nematode colonization and succession in disturbed soil using the Maturity Index

Nematode colonization and succession were studied over 60 weeks in field plots which had been disturbed by combinations of fumigation and manuring. It was expected that the Maturity Index, which is based on the composition of the nematode fauna, would decrease after disturbance, and subsequently increase with recovery and succession. The results showed that in all treatments, the initial colonization and growth were due to taxa scaled in colonizer-persister (c-p) group 1, resulting in a low Maturity Index. An increase in this index followed when these taxa were gradually replaced by c-p group 2 which thereafter remained the dominant group. C-p groups 3–5 developed only in the non-fumigated treatment. Manuring caused characteristic changes in the nematode fauna which could be described as a cycle, starting with an increase in enrichment opportunists (c-p 1), which were succeeded by general opportunists (c-p 2) which, in turn, were supplemented with persisters (c-p 3–5). It is expected that the same cycle will be observed as a result of other disturbances that cause temporary increase in food supply.     

真空预冷技术在子芋冷藏保鲜上的应用研究

将真空预冷技术配合塑料薄膜袋包装应用于冷藏保鲜子芋的预处理，研究不同的真空预冷处理工艺条件对子芋冷藏保鲜效果的影响，筛选最佳真空预冷工艺条件，并将保鲜效果同普通冷藏相比较。结果表明：采用真空室的真空度650 Pa，真空预冷终温4℃，预冷时间30 min进行真空预冷处理，并配合塑料薄膜袋包装的冷藏子芋比一般冷藏保鲜效果明显更好。表现在：冷藏30 d，外观饱满不皱皮，好果率提高19.5个百分点，失重率减少15.51个百分点，呼吸曲线明显下移，呼吸高峰推迟10 d出现，峰值减少20.2％。总糖保存率提高23.5个百分点。     

土荆芥果实挥发油的抑菌活性及其组分分析

为了比较土荆芥果实挥发油的熏蒸和触杀抑菌活性,测定了其对植物病原真菌菌丝生长和孢子萌发的熏蒸与触杀抑制作用,初步研究了其抑菌机制。结果表明,土荆芥果实挥发油对植物病原真菌具有强烈的抑制作用,熏蒸和触杀抑制作用均随着挥发油剂量增加而增强,随处理时间延长多呈减弱趋势。其对白菜黑斑病菌、小麦赤霉病菌、水稻胡麻斑病菌3种真菌菌丝生长的熏蒸和触杀抑制作用半数有效浓度(EC50)分别为17.4~22.1μL/L、103.9~241.7μL/L,对水稻胡麻斑病菌孢子萌发的熏蒸和触杀抑制作用EC50分别为28.8μL/L、789.3μL/L。土荆芥果实挥发油通过熏蒸方式作用于水稻胡麻斑病菌菌丝,使其细胞内含物渗漏;通过触杀方式作用于水稻胡麻斑病菌菌丝,随挥发油浓度升高和处理时间延长,相对电导率增加。GC-MS分析结果显示,土荆芥果实挥发油主要成分为2-蒈烯和驱蛔素。综合分析土荆芥果实挥发油的抑菌活性,熏蒸作用的效果优于触杀作用。     

复方丹参片不同干燥工艺的UPLC特征图谱及指标性成分含量

为优选适宜复方丹参片的干燥方法,探索微波真空干燥、真空干燥和烘箱干燥3种干燥工艺对丹参提取液的影响,采用UPLC法测定不同干燥方法的丹参提取液的UPLC特征图谱及指标性成分含量。结果表明:迷迭香酸、丹酚酸B、隐丹参酮、丹参酮ⅡA浓度分别在0.007~0.026 mg/mL,0.130~0.440mg/mL,0.002~0.009mg/mL,0.003~0.015mg/mL的线性关系良好(r≥0.999 1),平均回收率分别为99.30%(RSD=0.62%),96.41%(RSD=0.47%),100.58%(RSD=1.23%),102.84%(RSD=1.02%)。微波真空干燥、真空干燥和烘箱干燥后的指标性成分总含量分别为64.780 5 mg/g、64.501 7mg/g和58.990 0mg/g。3种干燥方法的UPLC特征图谱相似度达0.997以上。综合考虑微波真空干燥明显优于其他干燥方法。