Construction of a chromosome-assigned,sequence-tagged linkage map for the radish,Raphanus sativus L. and QTL analysis of morphological traits

The radish displays great morphological variation but the genetic factors underlying this variability are mostly unknown. To identify quantitative trait loci (QTLs) controlling radish morphological traits, we cultivated 94 F₄ and F₅ recombinant inbred lines derived from a cross between the rat-tail radish and the Japanese radish cultivar ‘Harufuku’ inbred lines. Eight morphological traits (ovule and seed numbers per silique, plant shape, pubescence and root formation) were measured for investigation. We constructed a map composed of 322 markers with a total length of 673.6 cM. The linkage groups were assigned to the radish chromosomes using disomic rape-radish chromosome-addition lines. On the map, eight and 10 QTLs were identified in 2008 and 2009, respectively. The chromosome-linkage group correspondence, the sequence-specific markers and the QTLs detected here will provide useful information for further genetic studies and for selection during radish breeding programs.     

浅析遥感影像图在森林资源二类调查中的应用

文章扼要阐述了卫星遥感影像图在森林资源二类调查中的作用、影像特征、成像规律及在使用卫星遥感影像图过程中应注意的问题。     

利用重组自交系定位棉花第22染色体短臂的纤维品质性状的QTL

 以TM-1的染色体片段代换系CSB22sh和TM-1杂交,构建了包含104个家系的重组自交系（RILs）群体。利用74对具有多态性位点的引物进行检测,构建了包含61个多态性位点,长度为76.93 cM的遗传图谱,平均标记间距离1.26 cM。利用此遗传图谱结合重组自交系群体4个环境下的5个纤维品质性状进行QTL定位,共定位出12个QTLs,解释性状表型变异的2.45%～21.11%;在1,2,3,4个环境中同时检测到的QTLs分别有9,1,1,1个。而利用4个环境平均值进行联合分析定位出个4个QTLs,纤维长度和纤维整齐度的QTLs均为2个,解释性状表型变异的14.37%～19.97%,并且纤维长度和整齐度的QTL在相同的位置。多环境下检测到的QTL可能对标记辅助选择有实际应用价值。     

TatukGIS控件在自动站资料显示及分析系统中的应用

为进一步利用自动气象站所观测的大量数据,以Delphi2010为开发环境,采用TalukGIs地理信息系统开发控件,通过查询所获取的各类自动观测数据结合矢量及栅格地图,实现了全市自动气象站资料的显示、色斑图绘制、雷达图叠加、数据分析等功能,为台站业务人员提供了一个较好的自动气象站数据分析平台及中小尺度灾害性天气监测平台。     

基于Flex与瓦片地图引擎的水资源调度系统设计与实现

针对目前水资源调度系统难以进行异步请求方式和兼容性的问题,提出采用Flex与瓦片地图引擎技术开发水资源调度系统的思路.介绍了Flex技术的应用架构和机制,分析了瓦片地图引擎的构造原理,构建了水资源调度系统的框架,设计了郑州市水资源调度系统的数据库模型、客户端和服务器端.以郑州市生态水系调度运行成果为基础,开发了郑州市生态水系调度运行软件系统.结果表明,Flex和瓦片地图技术可以实现水资源调度模块的可视化和网络平台化,为水资源调度的信息化提供了有效技术手段.     

农产品公司资产净利率影响因素分析与公司评价模型——以深圳市某农产品公司为例

以深圳市某农产品公司的财务数据为依据,对农产品公司的财务指标进行分析,寻求影响企业盈利的因素,构建多元统计模型,并对农业企业综合能力的评测提出一种新的方法,以期能够让公司更加有效地了解企业状况。     

基于TM遥感影像的林相图的编制与应用

探讨了如何以TM遥感影像为数据源 ,运用地理信息系统技术和遥感技术编制林相图的技术方法和过程 ,并对遥感图像处理、地图数字化、图像几何精校正和基于遥感的林相图的应用等问题作了详细论述     

Detection of edaphic discontinuities with ground-penetrating radar and electromagnetic induction

Quantification of edaphic properties which may regulate the spatial distribution of vegetation is often limited by the expense and labor associated with collecting and analyzing soil samples. Here we evaluate the utility of two technologies, ground-penetrating radar (GPR) and electromagnetic induction (EMI), for rapid, extensive and non-destructive mapping of diagnostic subsurface features and soil series map unit boundaries.Strong reflectance from fine-textured, near-surface soils obscured radar signal reflectance from deeper horizons at our field test site in the Rio Grande Plains of southern Texas, USA. As a result, ground-penetrating radar did not delineate known edaphic contrasts along catena gradients. In contrast, EMI consistently distinguished boundaries of soil map units. In several instances, gradients or contrasting inclusions within map units were also identified. In addition, the location and boundary of calcic or cambic-horizon inclusions embedded within a laterally co-extensive and well-developed argillic horizon were consistently predicted. Correlations between EMI assessments of apparent conductivity (ECa) and soil properties such as CEC, pH, particle size distribution and extractable bases were low (i.e., explained<6% of the variance), or non-significant. As a result, EMI has a high prospecting utility, but cannot necessarily be used to explain the basis for edaphic contrasts.Results suggest EMI can be a cost-effective tool for soil survey and exploration applications in plant ecology. As such, it is potentially useful for rapidly locating and mapping subsurface discontinuities, thereby reducing the number of ground truth soil samples needed for accurate mapping of soil map unit boundaries. An application, addressing hypotheses proposed to explain the role of edaphic heterogeneity in regulating woody plant distribution in a savanna parkland landscape, is presented.     

Effects of stream map resolution on measures of riparian buffer distribution and nutrient retention potential

Riparian ecosystems are interfaces between aquatic and terrestrial environments recognized for their nutrient interception potential in agricultural landscapes. Stream network maps from a broad range of map resolutions have been employed in watershed studies of riparian areas. However, map resolution may affect important attributes of riparian buffers, such as the connectivity between source lands and small stream channels missing in coarse resolution maps. We sought to understand the influence of changing stream map resolution on measures of the river network, near-stream land cover, and riparian metrics. Our objectives were: (1) to evaluate the influence of stream map resolution on measures of the stream network, the character and extent of near-stream zones, and riparian metrics; (2) to compare patterns of variation among different physiographic provinces; and (3) to explore how predictions of nutrient retention potential might be affected by the resolution of a stream map. We found that using fine resolution stream maps significantly increased our estimates of stream order, drainage density, and the proportion of watershed area occurring near a stream. Increasing stream map resolution reduced the mean distance to source areas as well as mean buffer width and increased the frequency of buffer gaps. Measures of percent land cover within 100 m of streams were less sensitive to stream map resolution. Overall, increasing stream map resolution led to reduced estimates of nutrient retention potential in riparian buffers. In some watersheds, switching from a coarse resolution to a fine resolution stream map completely changed our perception of a stream network from well buffered to largely unbuffered. Because previous, broad-scale analyses of riparian buffers used coarse-resolution stream maps, those studies may have overestimated landscape-level buffer prevalence and effectiveness. We present a case study of three watersheds to demonstrate that interactions among stream map resolution and land cover patterns make a dramatic difference in the perceived ability of riparian buffers to ameliorate effects of agricultural activities across whole watersheds. Moreover, stream map resolution affects inferences about whether retention occurs in streams or riparian zones.     

大白菜永久高密度分子遗传图谱的构建

 以大白菜高抗TuMV白心株系91-112和高感TuMV桔红心株系T12-19为亲本建立的小孢子培养DH系作为图谱构建群体, 构建了包含10 个连锁群、406 个标记位点的分子连锁图谱, 图谱总长度826.3 cM, 标记间的平均图距为2.0 cM, 连锁群数目和染色体数相等。每个连锁群上的标记数在7～111个之间, 连锁群的长度在26.4～156.1 cM的范围内, 平均图距在1.0～3.8 cM之间。该连锁图谱包括246个AFLP标记、135个RAPD标记、11个SSR标记和12个同工酶标记、1个SCAR标记和1个形态标记。