Sensitivity of four Yeasts to Fungicides and CO2 Concentrations and Their Antagonistic Ability in Combination with Fungicide to pathogenic Fungi in vitro

The yeasts Cryptococcus laurentii,Trichosporon pullulans,Rhodotorula glutinis and Trichosporon sp.were used to investigate their sensitivity to four fungicides and different concentrations of CO2,as well as their antagonistic ability to Penicilliumexpansum and Alternaria alternata in vitro when applied with fungicide.There were significant differences in sensitivity to the fungicides among the different yeasts(P = 0.05).R.glutinis was more sensitive to Deccocil,Iprodione and Stroby as compared to other yeasts.Combination antagonistic yeasts with fungicide could more significantly enhance biocontrol ability of the yeasts against the pathogenic fungi in vitro(P = 0.05).C.laurentii was the most effective antagonist among the four yeasts and could completely control spore germination of P.expansum and A.alternata when combined with Stroby at the concentration of 100 μL L-1.The yeasts,except R.glutinis,could grow well on nutrient yeast dextrose agar (NYDA) after 8 d incubation even at 20% CO2 concentration at 25℃.Particularly Trichosporon sp.showeda better adaptability to low temperature as compared to other antagonists.     

白细胞介素和干扰素γ对绵羊体外受精胚胎体外发育影响的研究

利用SOFM+0.8%BSA+1mM谷氨酰胺作为绵羊胚胎的培养液,分别添加LIF、IL-1β、IL-2、IL-6及IFN-γ,以研究LIF、IL-1β、IL-2、IL-6及IFN-γ对绵羊早期胚胎发育的影响。结果说明,LIF促进2～8细胞胚胎发育至桑椹胚,同时也能够促进桑椹胚向扩大囊胚和孵化囊胚发育。但IL-1β、IL-2、IL-6及IFN-γ对绵羊早期胚胎的发育无显著影响。     

Effect of Nitrogen Rate and Stubble Height on Dry Matter Yield,Crude Protein Content and Crude Protein Yield of a Sorghum–Sudangrass Hybrid[Sorghum bicolor (L.) Moench × Sorghum sudanense (Piper) Stapf.] in the Three‐Cutting System

In this study, the effects of nitrogen (N) rate (60, 120, 180 and 240 kg N ha^?1 applied in three equal dressings at seeding and after the first and second cuttings) and stubble height (7, 14 and 21 cm) on the dry matter (DM) yield, crude protein (CP) content, and CP yield of a sorghum–sudangrass hybrid [Sorghum bicolor (L.) Moench × Sorghum sudanense (Piper) Stapf., cv. Pioneer 988] in the three‐cut system was investigated. The N rate had no significant effect in the first and third cuttings, but in the second cutting DM yields increased significantly with increase in N rate. The highest yield of 9.1 t ha^?1 was obtained with 80 kg N ha^?1 for the average of 2 years at the second cutting, but no significant difference was found among the 40, 60 and 80 kg N ha^?1 rates. CP content and yield were not significantly affected by N rate at the first and third cuttings, but CP content and yield were significantly affected by application of N at the second cutting. Stubble height had a significant effect on CP content at the third cutting. However, it had no significant effect on CP content at the first and second cuttings. Stubble height had a significant effect on the CP yield at the first cutting, but no significant effect on CP yield at the second and third cuttings.     

猪肺炎支原体膜蛋白的电泳分析及免疫印迹检测

以猪肺炎支原体Z株为试验材料,采用SDS-PAGE和Western印迹对该菌膜蛋白进行了分析。研究表明:猪肺炎支原体经A_(26)液体培养基培养,15000 r/min离心收集菌体细胞,低渗超声法破膜获得膜制剂后,应用12.5％的凝胶对膜蛋白进行SDS-PAGE分离,在电泳图谱上呈现出36条蛋白带,相对分子质量范围为1.18×10~4～9.12×10~4。同时以膜制剂作为抗原,分4次免疫试验兔,心脏采血,提取抗血清,并分离纯化得到IgG,然后进行Western印迹法检测。其结果发现在SDS-PAGE分离得到的36种膜蛋白(或多肽)中,有6种蛋白具有免疫原性。     

生长抑素(SS)与硫氧还原蛋白(Thioredoxin)相融合生成的2种基因工程化融合蛋白SS-N/X和SS-N/S的表达特性

对基因工程化生长抑素（somatostatin,SS)-硫氧还原蛋白（Thioredoxin)融合蛋白SS－N／X和SS－N／S的表达产量、水溶性、Triton X-100对SS融合蛋白水溶性的影响、包涵体的溶解和复性特点以及SS的抗原性与免疫原性等特性进行了研究。结果显示，在30℃的低温下用IPTG诱导表达，SS－N／X融合蛋白主要以可溶性形式存在，占75．8％，色涵体仅占24．2％；而SS－N／S融合蛋白则主要以包涵体的形式存在，占81．1％，可溶性蛋白仅占18．9％；32C载体（pEG-32C)蛋白则居二者之间，可溶性蛋白与包涵体大约等比例出现。SS－N／S在低温下诱导可超量表达SS融合蛋白，其表达量约占菌体总蛋白的40．94％。0．5％ Triton X-100对融合蛋白的不溶性有非常显著的影响，几乎可使所有的SS－N／X融合蛋白和大部分SS－N／S融合蛋白及32C硫氧还原蛋白变成水溶性的。32C硫氧还原蛋白包涵体在尿素中的溶解度明显高于SS－N／S包涵体，2mol/L尿素可使近一半的32C硫氧还原蛋白包涵体溶解，而SS－N／S包涵体只有27．5％溶解；但在5mol/L尿素时，两者均可基本全部变为可溶性的。在SS融合蛋白包涵体溶解时加入还原剂二硫苏糖醇，可明显降低SS的抗原性。SS融合蛋白（水溶性融合蛋白和复性后的包涵体）具有良好的SS抗原性和免疫原性。由SS融合蛋白与弗氏不完全佐剂制成试验用疫苗，免疫BALB／c小鼠、仔猪和羔羊等动物，可显著提高免疫动物的增重。     

猪肺炎支原体黏附因子基因R1R2区的克隆及表达

根据GenBank登录的猪肺炎支原体232株P97基因和J株黏附因子基因设计了1对引物，以我国猪肺炎支原体Z株(强毒株)基因组DNA为模板，通过PCR方法扩增了该株黏附因子基因的部分序列。经序列分析后，重新设计了1对带有EcoRI和HindⅢ酶切位点的引物，并经引物的定点突变，PCR扩增了Z株黏附因子的R1R2区。扩增产物经双酶切后克隆到表达载体pET-32(a) 中。该重组质粒经酶切鉴定后，将其具有正确阅读框架的重组质粒转化到大肠杆菌BL21(DE3)感受态细胞，37℃下经IPTG诱导表达，得到相对分子质量约29000的融合蛋白，表达量约为11％。     

超临界CO_2流体处理工艺对木材渗透性的影响

以苯—乙醇为夹带剂,研究超临界CO2流体不同处理压力、温度、时间对福建产杉木、马尾松、檫木木材渗透性的影响。结果表明,在35 MPa内,处理压力的增大有利于3种木材渗透性的提高;杉木在处理温度为40℃时,渗透性的变化率达到最大,为493%,马尾松和檫木在处理温度为45℃时,渗透性的变化率达到最大,分别为479%和349%;较佳的处理时间为30 min。     

Estimating Combining Ability of 4x-2x Crosses in Cultivated Potatoes

Progenies derived from crosses between Solarium tuberosum and 2n pollen-Producing diploid hybrids, exhibit obvious hybrid vigor. The 2n pollen-producing clone can act as a bridge in crossing S. tuberosum and S. andigena with S. phureja. Populations from 4x-2x crosses show more unifomity and less segregation compared with that of 4x-4x crosses. The parent-offspring correlation for the traits, starch content and tuber number, is significant at 0.01 level. The regrssion equations are Y (mp-F1)=1.0 1.2x and Y (mp - F1) = 5.3 0.8x, respectively. The 2n pollen-producing clones play an impotant role in increasing tuber stach content. Estimates of the combining ability for the main yield components indicate that additive effect prodominatcs for such trais as plot yield, tuber weight per plant and starch content, whereas both additive and non-additive effects lay equal stress on mean tuber weight and non-additive effect is important for tuber number. In general, non-additive effect appears to be important in     

棉花纤维发育后期Mg2+,K+-ATP酶的活性分析

1989～1990年选用陆地棉(G.hirstum L.)北农1号品种和海岛棉(G.barbadenseL.)海7124品种为材料,对棉纤维发育后期的纤维素形成过程进行了研究。结果表明:①随着棉纤维的发育 Mg~(2+),K~+-ATP 酶活性逐渐下降。②Mg~(2+),K~+-ATP 酶活性的最适 pH 值为6.5。Mg~(2+),K~+ATP 酶活性随[Mg~(2+)],[K~+]和[NADH]/[NAD~+]的增大而变大。③Mg~(2+),K~+ATP 酶活性变化与还原性糖含量变化呈显著正相关(r=0.811~*),与纤维素含量变化呈极显著负相关(r=-0.900~(**))。④Mg~(2+),K~+-ATP 酶参与了纤维素的形成过程。     

基于图形旋转系统的渐进网格研究

拓扑简化可以为渐进网格带来更好的效果，但目前几乎所有的渐进网格方法都不支持拓扑简化，而且edge collapse和vertex split操作有时会产生非流形．针对这些问题，提出了基于图形旋转系统的渐进网格法．以基于图形旋转系统的数据结构和操作实现了渐进网格，用图形旋转系统的操作集合构建了edge collapse和vertex split操作．在此基础上，通过扩展新操作即可进行任意拓扑变化，从而实现拓扑简化，