Characterization of molecular variants of GnRH, induction of spermiation and sex reversal using salmon GnRH-A and domperidone in the protogynous diandric fish, Synbranchus marmoratus Bloch, (Teleostei, Synbranchidae)

This paper studies the molecular variants of gonadotropin-releasing hormone (GnRH) present in the brain of the protogynous swamp eel, Synbranchus marmoratus, and the effects of the administration of salmon GnRH analogue (sGnRH-A) and the dopamine receptor antagonist, domperidone (DOM) on final maturation and gamete release in this species. Evidence for the presence of two GnRH variants, sGnRH and cIIGnRH were obtained by reverse phase high-pressure liquid chromatography (RP-HPLC) and radioimmunoassay with different antisera. The effects of treatment with sGnRH-A+DOM were checked by three ways: oocyte and milt release by stripping, histological analysis of the gonadal tissue, and androgen serum levels at different times throughout the experiment. In males, spermiation was induced after three weeks of treatment. In the female group, sGnRH-A+DOM did not induce ovulation at the end of the experiment. Histological analysis of the gonads from the female group showed evidence of sex reversal. All the treated fish had elevated androgen serum levels from the third week, with respect to control fish. In all cases, serum estradiol levels were undetectable. These results suggest that treatment with sGnRH analog and DOM induce sex reversal in female and spermiation in males. This revised version was published online in July 2006 with corrections to the Cover Date.     

Effect of water temperature and growth variation on the sex ratio of experimentally reared eels

In a controlled experiment, elvers (Anguilla anguilla L.) were reared for 137 weeks at 17, 20 and 26°C. Most eels with macroscopically sexable gonads were males. The proportions of females were 14% of sexable individuals at 26°C and 7–8% at 17 and 20°C. During the first 15 weeks, 20–50% of initial numbers were lost, but this early mortality was not significantly size selective. In spite of a long term experiment, a significant number of the survivors never attained a sexable size. Nongrowers (7–9 cm) were still alive nearly three years after being caught as glass eels. Among the earliest fast growers, 5% females, or less, were found. The proportion of females increased in later grading groups, but the pattern of increase did not indicate that males and females have separated mean ranks in a fixed growth hierarchy. Water temperature influenced observed sex ratios, but probably not through direct influence on the sex differentiation. Some alternative hypotheses, dealing with population density and individual growth status, are discussed.     

The cytochrome P450 system of Atlantic salmon (Salmo salar): II. Variations in hepatic catalytic activities and isozyme patterns during an annual reproductive cycle

A group of Atlantic salmon (Salmo salar) was followed through their first year of maturation and spawning. At monthly intervals, starting with juvenile fish in December, 5–7 fish of each sex were killed, and liver and plasma were sampled. The last sampling point was of spawning fish in November a year later. Variables in the cytochrome P450 (P450) system were studied in hepatic microsomes, and estradiol 17 was measured in the plasma of females to assess the maturational status. The P450 1A1-mediated 7-ethoxyresorufin O-deethylase (EROD) started at high levels in winter, but decreased to non-detectable activities in pre-spawning females. Decreases, but not to the same extent, were also observed during this period in total cytochrome P450, cytochrome b₅, NADPH-cytochrome P450 reductase, and in the content of two immunochemically determined P450 isozymes. At the same time, LSI levels increased in maturing females (starting in July), and GSI levels increased in both sexes (starting in May). Sex specific differences were observed in pre-spawning fish in September and October, with levels of total P450, b₅, NADPH-cytochrome P450 reductase, EROD and P450 isozymes significantly lower in females. At the same time, plasma estradiol-17 levels reached peak values in females. The results point to the important role of sex steroids such as estradiol-17 as major factors in the regulation of final sexual maturation. However, this study also indicates that there may be estradiol-17 independent events of equal importance in the early stages of gonadal maturation that may involve the P450 system. The changes observed in the P450 system (as a major drug and steroid metabolizing system) of Atlantic salmon during sexual maturation may be of importance both in the endogenous transduction of hormonal signals, and as a pharmacological basis for designing therapeutic treatment of diseases in the aquaculture industry.Parts of this work were presented at the 5th International Symposium on Responses of Marine Organisms to Pollutants, April 1989 in Plymouth, United Kingdom (Larsen and Goks\/oyr 1989).     

中华绒螯蟹副性征的研究

在解剖、测量性成熟和未成熟中华绒螯蟹外部形态和性腺之基础上,试用“双百分率法”和“差异显著性检测法”对中华绒螯蟹的副性征进行了研究。结果表明,性成熟后的雌、雄中华绒螯蟹均具有较明显的副性征。     

Effects of (D-Ala6, Pro9N ethylamide) — LHRH on plasma levels of gonadotropin, 17α,20β-dihydroxy-4-pregnen-3-one and testosterone in male goldeye (hiodon alosoides Rafineque)

Male goldeye were treated with (D-Ala⁶, Pro⁹-N ethylamide) — LHRH (LHRH-A) in saline or a silastic pellet (100 µg.kg^–1 body weight) and changes in plasma gonadotropin (GtH), 17,20-dihydroxy-4-pregnen-3-one (17,20P) and testosterone (T) levels were determined by radioimmunoassay. LHRH-A increased plasma GtH levels, with the response to LHRH-A in saline being of much greater magnitude and duration than the response to silastic pellet implants. Seasonal differences were found in the response to LHRH-A. Spermiated fish were the most responsive, recrudescing fish the least, and regressed fish displayed an intermediate response. Plasma 17,20P levels were elevated in response to LHRHA in fish of all sexual stages although the magnitude of the increase was not related to the magnitude of the increase in GtH levels. Treatment with LHRH-A also resulted in a transitory increase in plasma T levels. The endocrine control of GtH and steroid secretion in goldeye is discussed in relation to studies in cyprinids and salmonids. Address for reprint requests: Department of Zoology, University of Alberta, Edmonton, Alberta T6G 2E9 Canada.     

2013年杭州市萧山区松墨天牛成虫林间种群动态的监测研究

2013年4—10月对浙江省杭州市萧山地区南部马尾松与松阔次生混交林林中松墨天牛的种群数量动态进行了监测,结果表明,松墨天牛在萧山地区南部松林中1年只发生1代,成虫活动期为5月中旬至9月下旬,6月下旬为成虫羽化高峰期,2个监测点所诱获的松墨天牛成虫种群个体的雌、雄性比为1.51:1和1.73:1。     

蓖麻内源乙烯释放率与性型关系初步研究

为了探究内源乙烯与蓖麻性别的关系,以蓖麻雌性自交系 YC2-S 及其姊妹两性自交系 YC2-F 为材料, 用气相色谱法测定了不同时期叶片内源乙烯释放率的变化。 结果表明院从三叶期到开花期,雌性系内源乙烯释放率呈 倒野S冶型变化,即从三叶期的 137. 9 滋L/ kg 窑 h下降到五叶期的 17. 5 滋L/ kg 窑 h,然后缓慢上升,现蕾期达到 40. 9 滋L/ kg 窑 h,开 花期再降至 26. 9 滋L/ kg 窑 h;两性系呈野U冶型变化,即从三叶期的 149. 8 滋L/ kg 窑 h下降到五叶期的 18. 4 滋L/ kg 窑 h,然后持 续上升,到开花期达到 226. 3 滋L/ kg 窑 h。 二者在六叶期以前没有差异,六叶期后,前者先升后降,后者持续上升,现蕾期 差异显著、开花期差异极显著。 证明乙烯是蓖麻性型决定的关键因素之一。     

性外激素诱发中华乌塘鳢产卵的初步研究

在陶瓷人工产卵管道内吊挂性外激素的研究表明,卵巢提取液对雄鱼的吸引作用大于雌鱼,而精巢和贮精囊提取液对雌鱼的吸引作用则大于雄鱼,17α-P、17α,20β-P和PGE2对雌鱼和雄鱼均具有相似的吸引作用。雌鱼在管道内的产卵次数、产卵量以及成熟卵的受精率均与产卵管道内吊挂的性外激素种类有关,17α,20β-P和PGE2两个实验组产卵次数最多,其中PGE2实验组的产卵量和受精率最高。17α-P在贮精囊和精巢提取液中的含量显著高于卵巢提取液(P<0.05);贮精囊提取液中PGE2和PGF2α的含量分别显著高于精巢提取液和卵巢提取液(P<0.05);提取液中PGE2和PGF2α的含量均依次为贮精囊提取液>精巢提取液>卵巢提取液。卵巢提取液刺激雄鱼所引起的平均EOG高于雌鱼,而精巢和贮精囊提取液刺激雌鱼所引起的平均EOG高于雄鱼,雌鱼和雄鱼对17α-P、17α,20β-P、PGE2和PGF2α刺激所产生的平均EOG,以PGE2为最高。     

Immunolocalization of sex steroid receptors in the epididymis and ductus deferens of immature and mature Japanese Quail, Coturnix Japonica

The goal of this study was to determine whether in the Japanese quail the male genital tract contains receptors for progesterone, androgen and estrogen (PR, AR and ER, respectively), which have significant roles in reproductive functions, and whether their localization changes during sexual maturation. The epididymis and ductus deferens (middle and ampulla regions) of immature (approximately 30-day-old) and mature male Japanese quail were collected and frozen sections of them were immunostained for PR, AR and ER. The immunoreaction products for AR and PR were found in the nuclei of epithelial cells in the efferent ductules, epididymal duct, and the middle and ampulla regions of the ductus deferens of mature and immature birds. In the mature birds, the epithelial cells of the efferent ductules, epididymal duct, and the middle and ampulla regions of the ductus deferens were positive for ER, although some of the cells in the ductus deferens were negative. The epithelial cells of the ductules in the epididymis stained positive for ER, but the immunoreactions were negligible in the ductus deferens of immature birds. These results suggest that the epididymis and ductus deferens in quail possesses PR, AR and ER receptors. Each receptor is expressed before sexual maturation, although enhancement of ER expression may occur during maturation.     

枣粘虫性行为时辰节律的研究

本文利用腺体提取、瓶内收集、触角电位和田间试验等方法对枣粘虫产生和释放性信息素时辰节律进行了研究。其中瓶内收集性信息素的方法,在研究昆虫释放性信息素时辰节律方面报道很少,更重要的是,此法在我们的实验中取得了比较满意的效果。研究结果表明:(1)羽化后1—6天的提取物产生的触角电位无明显差异,说明性信息素在此期间的产生与日龄无关(2)腺体中性信息素的含量高峰在凌晨4点,而从体内往体外释放的高峰在早晨7点。(3)利用处女雌蛾不同时辰的诱蛾量的田间试验也进一步证明了枣粘虫产生和释放性信息素与交配行为在时辰节律上的同步性。