全文获取类型
收费全文 | 191篇 |
免费 | 9篇 |
国内免费 | 7篇 |
专业分类
林业 | 4篇 |
农学 | 3篇 |
综合类 | 39篇 |
农作物 | 6篇 |
水产渔业 | 8篇 |
畜牧兽医 | 129篇 |
园艺 | 1篇 |
植物保护 | 17篇 |
出版年
2023年 | 2篇 |
2022年 | 4篇 |
2021年 | 10篇 |
2020年 | 10篇 |
2019年 | 7篇 |
2017年 | 2篇 |
2016年 | 6篇 |
2015年 | 6篇 |
2014年 | 16篇 |
2013年 | 7篇 |
2012年 | 11篇 |
2011年 | 13篇 |
2010年 | 11篇 |
2009年 | 17篇 |
2008年 | 6篇 |
2007年 | 3篇 |
2006年 | 7篇 |
2005年 | 4篇 |
2004年 | 1篇 |
2003年 | 5篇 |
2002年 | 8篇 |
2001年 | 6篇 |
2000年 | 4篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 5篇 |
1991年 | 2篇 |
1989年 | 4篇 |
1987年 | 3篇 |
1986年 | 2篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1980年 | 4篇 |
1979年 | 1篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有207条查询结果,搜索用时 31 毫秒
91.
92.
Yun Sang CHO Sang Eun LEE Jong-Tae WOO Jinsik OH Hwan Won CHOI Jin Hyeok KWON Jeong-Tae KIM Gunwoo HA Sukchan JUNG 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(11):1631
Bovine tuberculosis (bTB) is a zoonosis caused by Mycobacterium bovis. Test-and-cull protocols and gross pathological examinations of abattoir animals as well as milk pasteurization have been implemented to prevent the spread of tuberculosis from animals to humans worldwide. Despite the importance of precise and rapid diagnostic tests, conventional methods including intradermal skin tests and γ-interferon assays are limited by the high rate of false-negative results for cattle in the late infectious stage and due to laborious and time-consuming procedures. Therefore, antibody detection methods such as enzyme-linked immunosorbent assay (ELISA) are urgently needed to supplement the established approaches and expand the diagnostic window. This study was conducted to develop a bTB ELISA by evaluating recombinant and native proteins and various assay parameters. We produced recombinant MPB70 and SahH (M70S) and a native 20-kDa protein (20K) and optimized the ELISA protocol. The 20K ELISA showed 94.4% sensitivity and 98.2% specificity with an optimal sample-to-positive ratio cut-off of 0.531. The sensitivity and specificity of M70S ELISA were 94.4% and 97.3%, respectively, with an optimal sample-to-negative ratio cut-off of 1.696. Both assays showed acceptable diagnostic efficiency and could be used for bTB diagnosis in combination with established methods for herd screening and to expand the diagnostic window. 相似文献
93.
94.
Sylvie Priou R. Torres Alicia Villar Liliam Gutarra F. De Mendiburu 《Potato Research》2001,44(4):349-358
Summary A sampling strategy was evaluated in the Andean highlands of Peru to optimise the detection ofRalstonia solanacearum in seed tubers harvested from symptomless crops. A sensitive and specific serological method developed at CIP was used to
detect the pathogen in latently infected tubers. Optimum sample size was evaluated for symptomless crops after analysing various
numbers of composite samples and using a binomial distribution model to calculate the detection probabilities.R. solanacearum was detected in all lots from fields with visible symptoms, so validating the detection technique. About half of the seed
lots from apparently healthy fields at altitudes of up to 3,100 m were found positive for the pathogen.R. solanacearum was detected with 99% probability in samples of 350 tubers from seed lots from symptomless crops. This number of seed tubers
could feasibly be processed in a seed-health test without incurring too high a cost for labour and materials. 相似文献
95.
为了解猪流感病毒(SIV)在我国的流行情况,本研究对2011年~2012年从不同省市重点地区屠宰场、规模化猪场和个体养殖户采集猪血清样品13044份,以经典H1N1 (CS)、类禽H1N1 (EA)、甲型H1N1(pdm/09)、H3N2、H5N1、H9N2亚型SIV抗原并采用血凝抑制(HI)方法检测.结果显示,2011年~2012年我国猪血清中类禽H1N1亚型SIV抗体平均阳性率比其他亚型高,分别为42.24%和45.89%,2011年甲型H1N1亚型SIV血清抗体平均滴度(GMT)比其他亚型高,GMT为53.32,2012年经典H1N1 GMT比其他亚型高,GMT为73.2.结果表明2011年~2012年我国猪群中H1N1亚型SIV感染较为普遍. 相似文献
96.
In this study, to evaluate the influence of strangles vaccination on serological test
results, we investigated the changes in strangles serum antibody levels in horses after
vaccination and subsequent intranasal challenge with S. equi. The horses were vaccinated
for strangles with either a component vaccine (Group C) or a live vaccine (Group L). We
measured changes in strangles serum antibody levels weekly for 20 weeks after vaccinating
horses twice for strangles over a 3-week interval, and for 7 weeks after intranasal
challenge with S. equi in the same horses. Serum antibody responses to the
proline-glutamic acid-proline-lysine (PEPK) antigen with five repetitions (PEPK-5R) were
higher at all times (up to 2.4-fold) following vaccination in Group C than in Group L, and
the value peaked at 2.9-fold above the initial value after the second vaccination in Group
C horses. However, the value was lower than that in horses infected with S. equi, and it
gradually decreased, reaching the initial (week 0) value by the 15th week. Serum antibody
responses to PEPK-5R after challenge with S. equi increased in both groups of horses, but
the value tended to be lower than that reported for unvaccinated horses. In addition, the
average value in Group C was 2.6-fold higher than that of Group L. These results suggest
the serum antibody responses of horses infected with S. equi varies according to the type
of vaccine with which they have been vaccinated. Although the serological diagnostic test
for strangles in which PEPK-5R is used as an antigen is effective for the investigation of
serum antibodies to strangles in vaccinated horses, the present data suggest it is
necessary to consider the vaccination history when interpreting the results. 相似文献
97.
Tanja Opriessnig John R. Prickett Darin M. Madson Hui-Gang Shen Nicole M. Juhan Roman M. Pogranichniy Xiang-Jin Meng Patrick G. Halbur 《Veterinary research》2010,41(3)
Long-term PCV2 infection and/or concurrent infection with genotypes PCV2a and PCV2b may play a role in the development of clinical porcine circovirus-associated disease (PCVAD). To evaluate this premise, 24 11-week-old specific pathogen-free (SPF) pigs were randomly assigned to 1 of 4 treatments: negative controls, a single inoculation with PCV2a, single inoculation followed by re-inoculation with a homologous PCV2a strain, or repeated inoculations with heterologous strains (PCV2a, PCV2b). Pigs were evaluated for clinical signs daily through 140 days post inoculation (dpi). Serum samples were collected every other day from dpi 0 through 14 and weekly thereafter. PCV2-inoculated pigs were viremic by dpi 2 and 13 of 18 pigs remained viremic at 140 dpi. No statistical differences in the onset, level, or duration of PCV2 viremia were detected among treatment groups. Anti-PCV2 antibodies were detected between 14 and 28 dpi and were present through 140 dpi without statistical differences in antibody response among treatment groups. In the current study, pigs had extended viremia combined with detectable tissue PCV2 antigen levels despite the presence of high levels of anti-PCV2 antibody; however, no clinical disease was observed. 相似文献
98.
河南省奶牛病毒性腹泻-黏膜病血清学调查 总被引:2,自引:0,他引:2
摘要: 为了解河南省奶牛病毒性腹-黏膜病的发生情况,2009年5月对河南省的豫东、豫西、豫南、豫北和郑州市五区域20个奶牛小区的355头奶牛,随机采血,采用ELISA方法,检测奶牛病毒性腹泻-黏膜病血清抗体,结果显示20个奶牛小区有17个奶牛小区感染有病毒性腹泻-黏膜病病毒,场感染率为85%,各小区奶牛病毒性腹泻-黏膜病检测阳性率在0-88.24%,血清阳性率平均为53.8%,表明该病在河南省奶牛中感染率较高。 相似文献
99.
番茄环纹斑点病毒(Tomato zonate spot virus, TZSV)是番茄斑萎病毒属的一个新种,对云南番茄、辣椒生产造成严重危害。用RT-PCR 从感病番茄中扩增得到长度为837 bp TZSV 的核壳体蛋白基因(N 基因),将其克隆到原核表达载体pET-28a( + ),获得重组原核表达载体pET-TZSV-N,在大肠杆菌BL21 中表达,SDS-PAGE 分析表明,该载体高效表达33kDa 的融合蛋白;以纯化的融合蛋白作为抗原免疫家兔制备TZSV 核壳体蛋白(N 蛋白)的多克隆抗体,间接酶联免疫测定(ID-ELISA)表明效价为1 / 6 000;Western blot 分析表明,该抗血清能与西瓜银色斑驳病毒(WSMoV)血清组的辣椒褪绿病毒(CaCV)反应,而与番茄斑萎病毒(TSWV)、凤仙花坏死斑病毒(INSV)无血清交叉反应,说明获得的抗血清能用于WS-MoV 血清组成员的检测,TZSV 属于WSMoV 血清组成员。 相似文献
100.
为了解青海省民和县奶牛布鲁氏菌病的分布和流行情况,净化布鲁氏菌病,对来自12个乡镇的10308份奶牛血清采用试管凝集反应试验(SAT)进行了血清学检测,检出阳性血清23份,阳性率为0.223%,表明青海民和地区存在奶牛布鲁氏菌的感染。 相似文献