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51.
AIM: To explore the regulatory mechanism of nerve growth factor (NGF) on neurokinin A in the experimental asthmatic guinea pig. METHODS: Radioimmunoassay was used to determine the alteration of neurokinin A levels in the lower respiratory tract and visceral sensory afferent sites while NGF was absent (inhalation of NGF antibody through nasal cavity) in the asthmatic guinea pig. RESULTS: The contents of neurokinin A in the trachea, bronchus, lung, C7-T5 spinal ganglia and the correspondent spinal dorsal horn, nodose ganglia and solitary nucleus area in the experimental asthmatic guinea pig with the absent of NGF in the respiratory tract were much lower than those in the asthmatic and control groups (P<0.01). CONCLUSION: NGF upregulated the contents of neurokinin A in the lower respiratory tract and visceral sensory sites of the experimental asthmatic guinea pig, and both might be involved in the pathogenesis of asthma. 相似文献
52.
53.
AIM: To examine the expression and distribution of tumor necrosis factor-α (TNF-α), tumor necrosis factor receptor I (TNFR I) and apoptosis in oral lichen planus, and evaluate their roles and relation in the oral lichen. METHODS: Immunohistochemical technique and TUNEL were employed to study the expression of TNF-α, TNFR I and apoptosis in 50 cases of oral lichen planus and 10 normal oral mucosa specimens. RESULTS: Compared with the normal control group, TNF-α expression was upregulated in mononuclear cells in lamina propria and decreased in keratinocytes in oral lichen planus lesion (P<0.05). On the contrary, TNFR I expression was increased in keratinocytes and decreased in lamina propria in oral lichen planus lesion (P<0.05). The increased apoptosis index in keratinocytes and the decreased apoptosis index in lamina propria were found in oral lichen planus (P<0.05). CONCLUSION: The accelerated apoptosis of keratinocytes and the inhibition of lymphocytes apoptosis may contribute to the formation and progression of oral lichen planus. 相似文献
54.
AIM: To study the role of liver in immune regulation in experimental endotoxemia. METHODS: 17 castrated male goats were subjected to simultaneously installing catheters in jugular, hepatic and portal veins by surgery. Four days later, lipopolysaccharide (LPS) was infused in term of three groups as followings: In group ①, LPS of 20 EU (endotoxin unit, EU)·kg-1 was infused into portal vein; In group ②, LPS of 20 EU·kg-1 was infused into jugular vein and LPS of 1 500 EU·kg-1 infused into jugular vein in group ③. Before and after infusion, blood samples were collected from the three veins through the catheters for 8 h.The plasma levels of TNF-α were measured by RIA. RESULTS: In group ①, the plasma TNF-α levels of hepatic and portal vein rose to peak value at 5 h, but that of the jugular vein did not changed. In group ②, the plasma TNF-α levels in hepatic vein rose to peak value at 3 h. The TNF-α levels of jugular vein rose to peak value at 1 h and the one in portal vein enhanced continuously between 0-8 h. In group ③, the plasma TNF-α levels in jugular, hepatic and portal vein rose to significant peaks at 1 h simultaneously. CONCLUSION: During experimental endotoxemia,liver showed different dynamic characteristics in TNF-α secretion according to the pathway and doses of LPS delivery. 相似文献
55.
AIM: To investigate inhibition of K562 cell growth by antisense drug targeted VEGF mRNA. METHODS: X7, 20-mer antisense sequences were selected, synthesized and modified with phosphorothioate. The drug was transfected into K562 cells in the present of lipofection. Cell growth was assayed by trypan blue dye exclusion assay and MTT. The level of VEGF protein in the media was determined by ELISA. The morphology of apoptotic cells were observed by Giemsa staining, and the propotion of apoptotic cells was detected by flow cytometry. RESULTS: The antisense drug inhibited growth of K562 and downregulated expression of VEGF protein significantly, compared with Scrambed control group and showed dose-dependent relation. Signs of apoptosis of K562 cells were not observed. CONCLUSION: Inhibition of K562 cell proliferation, but not cells apoptosis induction is the mechanism of inhibing growth of K562 cells by antisense drug targeted VEGF mRNA. At same time, VEGF has function of promoting K562 cell proliferation, and VEGF mRNA may be a new target attached by drugs. 相似文献
56.
AIM: To investigate the role of nitric oxide synthase (NOS), soluble guanylyl cyclase (sGC) and protein kinase C (PKC) signaling in tumor necrosis factor-α (TNF-α)-induced cardioprotection against hypoxia/reoxygenation (H/R) injury. METHODS: Neonatal rat ventricular myocytes were pretreated with TNF-α or sodium nitroprusside (SNP) or L-arginine (L-Arg), respectively, for 12 h and then subjected to continuous hypoxia for 12 h, followed by reoxygenation for 6 h. The manganese superoxide dismutase (Mn-SOD) activity of the cells was measured after H/R. Myocyte injury was determined by the release of lactic dehydrogenase (LDH). RESULTS: TNF-α (105 U/L) significantly increased the Mn-SOD activity and decreased release of LDH from ventricular myocytes. The cardioprotection against H/R injury was induced by the pretreatment with SNP (5 μmol/L) or L-Arg (5 mmol/L), which was blocked by ODQ (10 μmol/L), the specific sGC inhibitor, and Chel (5 μmol/L), the specific PKC inhibitor. Pretreatment with L-NAME (100 μmol/L), ODQ, Chel, antoxidant 2-MPG (400 μmol/L) or tyrosine kinase inhibitor genistein (50 μmol/L) attenuated the increased Mn-SOD activity and reduced LDH level induced by TNF-α. CONCLUSION: The results suggest that NO may play a role in TNF-α-induced cardioprotection, which is mediated by sGC and PKC. 相似文献
57.
赤峰市松山区土地利用动态变化及其原因分析 总被引:8,自引:6,他引:8
以TM遥感资料为信息源 ,利用 3S技术手段 ,对内蒙古自治区赤峰市松山区 1987,1993,2 0 0 0年的土地利用变化进行了分析。结果表明 ,在不同年度内 ,其主要的土地利用类型为耕地、草地和林地。土地利用类型的总量变化也以这 3种土地利用类型和建设用地变化最大。而从年变化速率看 ,1987- 2 0 0 0年 ,以建设用地的增加和未利用土地类型的减少最为快速。从土地利用类型转化的方向看 ,以耕地、草地和林地相互之间的转化最为频繁。从土地利用类型的空间稳定性看 ,耕地的稳定性最高 ,其次为草地 ;而最不稳定的土地利用类型为未利用土地。利用相关分析所作的土地利用变化动因显示 ,该地区土地利用变化的驱动因素主要是社会与经济因素 ,其中以总人口、农业总产值、粮食作物产量总计、农牧民人均纯收入、化肥使用面积和化肥实物量等因子的作用最大 ,而自然因素对该地区土地利用变化的影响极小。 相似文献
58.
内蒙古沙尘暴的成因、趋势及其预报 总被引:2,自引:0,他引:2
本文利用内蒙古1961~2001年的天气气候资料,对内蒙古中西部地区沙尘暴作了统计分析,阐述了沙尘暴的危害并给出了沙尘暴的基本定义。分析了引起沙尘暴的天气和气候因子的变化趋势,研究了他们对沙尘暴的影响,结果表明近40年内蒙古的沙尘暴总体呈减少趋势,但从1998年开始有所增加;沙尘暴的空间分布以阿拉善盟偏北地区为最高发区;降水、气温、大风、寒潮、北半球极涡、西太平洋副热带高压、亚洲西风环流、东亚大槽和南方涛动等天气和气候因素均对该地区沙尘暴的发生有不同程度的影响。 相似文献
59.
Characterizing historical and modern fire regimes in Michigan (USA): A landscape ecosystem approach 总被引:4,自引:0,他引:4
Cleland David T. Crow Thomas R. Saunders Sari C. Dickmann Donald I. Maclean Ann L. Jordan James K. Watson Richard L. Sloan Alyssa M. Brosofske Kimberley D. 《Landscape Ecology》2004,19(3):311-325
We studied the relationships of landscape ecosystems to historical and contemporary fire regimes across 4.3 million hectares in northern lower Michigan (USA). Changes in fire regimes were documented by comparing historical fire rotations in different landscape ecosystems to those occurring between 1985 and 2000. Previously published data and a synthesis of the literature were used to identify six forest-replacement fire regime categories with fire rotations ranging from very short (<100 years) to very long (>1,000 years). We derived spatially-explicit estimates of the susceptibility of landscape ecosystems to fire disturbance using Landtype Association maps as initial units of investigation. Each Landtype Association polygon was assigned to a fire regime category based on associations of ecological factors known to influence fire regimes. Spatial statistics were used to interpolate fire points recorded by the General Land Office. Historical fire rotations were determined by calculating the area burned for each category of fire regime and dividing this area by fifteen (years) to estimate area burned per annum. Modern fire rotations were estimated using data on fire location and size obtained from federal and state agencies. Landtype Associations networked into fire regime categories exhibited differences in both historical and modern fire rotations. Historical rotations varied by 23-fold across all fire rotation categories, and modern forest fire rotations by 13-fold. Modern fire rotations were an order of magnitude longer than historical rotations. The magnitude of these changes has important implications for forest health and understanding of ecological processes in most of the fire rotation categories that we identified.This revised version was published online in May 2005 with corrections to the Cover Date. 相似文献
60.
AIM: To investigate the affected proteins by tumor necrosis factor (TNF)-α in endothelial cells, and further explore the potential molecular mechanism of TNF-α on endothelial cells. METHODS: Nitric oxide (NO) production in the cultured human umbilical vein endothelial cells (HUVECs) was measured by a NO assay kit. Proteomic alterations were analyzed using two-dimensional electrophoresis, and peptide mass fingerprinting with matrix-assisted laser desorption/ionization-time of flight mass spectrometry. RESULTS: NO production in HUVECs decreased significantly after TNF-α treatement. Proteomics analysis showed 21 protein spots were changed including 9 spots that were increased and 11 spots that were decreased after TNF-α stimulation, and 1 spot was only detected in TNF-α activated cell gels. CONCLUSIONS: Decreased the expression of ecNOS by TNF-α might result in decreased NO production. Up-regulated MAP/ERK kinase 3 expression might imply that TNF-α activates the expression of adhesion molecules. Cytoskeletal protein actin is also involved in TNF-α injuried HUVECs. Proteomic analysis can find some clues for identifying new potential target of TNF-α. 相似文献