视网膜母细胞瘤109例分析

目的：分析视网膜母细胞瘤的发病特点和诊治规律。方法；收集本院近２３ａ来的１０９例ＲＢ病毒，对其临床特点，病理表现，治疗，预后及家系调查作一回顾性分析。结果：７７．１％的病例就诊年龄小于５岁。２２例为双眼发病，９例具有肿瘤家族史。１０９例中以晚期虱阳多见，病理检查大多可见有视神经和巩膜浸润。     

Sequential expression of myogenic regulatory factors in bovine skeletal muscle and the satellite cell culture

Myogenic regulatory factors (MRFs) are important in the control of skeletal muscle development. To understand myogenic regulation by MRFs in bovine adult muscle cells, their expressions, namely that of Myf5, MyoD, myogenin, and MRF4 in the biceps femoris muscle (BF) and in the satellite cell culture, were analyzed by RT-PCR. In the BF, all four MRFs were expressed and in particular, myogenin and MRF4 were strongly expressed, whereas Myf5 was faintly expressed. The satellite cells prepared from the BF expressed Myf5, but only a trace of MyoD, at day 9 of culture. During the growth of the cells to day 14, the MyoD and myogenin expressions gradually increased, and that of MyoD expression reached its maximum at the confluence of the culture. After induction of myogenic differentiation by a serum-free medium at day 14, Myf5 expression gradually decreased, and the up-regulated expression of MyoD was suppressed, whereas myogenin expression continued to increase sharply. Following the myogenin expression, MRF4 also drastically increased toward the myotube formation of the cells. When huge myotubes were formed at day 18, Myf5 was expressed at a low level, whereas the MyoD expression remained at a moderate level.     

Analysis of puroindoline a and b sequences from Triticum aestivum cv. `Penawawa' and related diploid taxa

Flanking sequences of the puroindoline a(pinA) and puroindoline b (pinB) genes from Triticum monococcum, T. urartu, Aegilops speltoides and A. tauschii were obtained by inverse PCR. Two accessions from each of these related diploid taxa were sequenced, and the sequences compared to those of the soft hexaploid wheat cultivar `Penawawa'. As expected from the origin of the D-genome in hexaploid wheat, the highest sequence identity was observed for the A. tauschii sequences, whereas the sequences for A.speltoides were the most divergent. The promoter sequences were further analyzed for putative regulatory elements, and the possible significance of several highly conserved sequence motifs is discussed. Western blots of Triton X-114 extracted proteins separated by SDS-PAGE confirmed the accumulation of pinA and pinB gene products in the endosperm of all the germplasm lines studied. The deduced amino acid sequences for the related diploid taxa do not imply any major structural changes as compared to the wild-type T. aestivum puroindolines, and is therefore in agreement with the soft endosperm texture of these species. This revised version was published online in August 2006 with corrections to the Cover Date.     

Molecular functions of genes related to grain shape in rice

Because grain shape is an important component of rice grain yield, the discovery of genes related to rice grain shape has attracted much attention of rice breeding programs. In recent years, some of these genes have been cloned and studied. They have been found not only regulate grain shape by changing the shape of the spikelet hull, but also regulate endosperm development through control of cell division using different molecular mechanisms. In this paper, we review the recent research on genes related to rice grain shape and their possible regulatory mechanisms.     

The Importance of the Bolivian Wild Potato Species in Breeding for Globodera pallida Resistance

Screening for resistance to the potato cyst nematode, Globodera pallida, in potatoes from. Bolivia, was carried out in 1983 and 1984, using a mixture of four nematode populations representing pathotypes Pa₁, Pa₂ and Pa₃ From the 66 accessions of 17 species and subspecies evaluated, highly resistant genotypes were identified in 21 accessions from seven species. All had Pf/Pi values of 2 or less, whereas the susceptible control, Solanum tuberosum cv. ‘Disiree’ had Pf/Pi values of more than 2G in both tests. Two diploid wild species, S. brevicaule and S. leptophyes, showed the best resistant. The geographical distributional of resistant populations and the evolution of resistance in wild potato populations are discussed.     

The nature of the Bolivian weed potato species Solanum sucrense Hawkes

Summary Two theories for the origin of the Bolivian weed potato species Solanum sucrense Hawkes have been tested. The first was that it had an allopolyploid origin as a hybrid of the cultivated tetraploid S. tuberosum ssp. andigena (Juz. et Buk.) Hawkes with the wild/weed tetraploid cytotype of S. oplocense Hawkes. The second hypothesis postulated that it was a cross of the weed diploid species S. sparsipilum (Bitt.) Juz. et Buk. with S. oplocense.Synthetic hybrids of S. tuberosum ssp. andigena x S. oplocense and of S. sparsipilum x S. oplocense have been compared morphologically with S. sucrense accessions. Their crossability has also been investigated.The crossability and morphological studies strongly suggested that the former hypothesis was the more likely. This was borne out by observations made on the 1974 University of Birmingham Expedition when segregating populations of S. sucrense and S. oplocense were collected growing with feral S. tuberosum ssp. andigena.The evidence from this study indicates that the S. tuberosum ssp. andigena x S. oplocense hybrid has formed the basis of the S. sucrense gene pool. It is suggested that this hybridogenic taxon be maintained under the name Solanum x sucrense.The clarification of the taxonomic relationships of these three species will be of interest to Globodera resistance breeders, in view of the fact that these taxa are resistant to various pathotypes of the golden nematode.     

Ecological lessons from long-term studies in experimental forests: Ponderosa pine silviculture at Pringle Falls Experimental Forest, central Oregon

The United States Department of Agriculture (USDA) Forest Service celebrated the 100th anniversary of its network of experimental forests in 2008. For a century, the network of experimental forests have contributed immensely—both in the US and around the world—to the practical understanding of the environment and to the formation of management approaches and policies that affect our use of forests and the natural resources they contain. Experimental forests provide places for long-term science and management studies in major vegetation types across the 789,140 km² of public land administered by the Forest Service. They also provide an incredible wealth of records and knowledge of environmental change in natural and managed forest ecosystems across the United States. Pringle Falls Experimental Forest (Pringle Falls), southwest of Bend, Oregon, is the oldest experimental forest in the Pacific Northwest and is the site of some of the earliest forest management and silviculture research in this region. Research at this site began in 1914, and it was formally established as part of the national network of experimental forests in 1931 as a center for silviculture, forest management, and insect and disease research in ponderosa pine forests east of the Oregon Cascade Range. As part of the celebration of the Centennial Anniversary of the network of experimental forests, it is timely to examine a few of the ecological lessons that have resulted from long-term studies at Pringle Falls. Two different yet interconnected themes are traced: (1) management of existing old-growth ponderosa pine; and (2) management of young or immature ponderosa pine. Examples of ecological lessons, drawn from long-term studies established or followed by James W. Barrett, Matt D. Busse, Patrick H. Cochran, Walter G. Dahms, F. Paul Keen, Ernest L. Kolbe, Edwin L. Mowat, William W. Oliver, Ram Oren, Chester T. Youngberg, and Jianwei Zhang illustrate how work at Pringle Falls has both pursued and influenced societal demands for forest management strategies, and how this trajectory has cycled back to the themes under which the experimental forest was first established. Finally, these two themes are integrated as drivers for new landscape-scale long-term research at Pringle Falls, designed to evaluate the effects of thinning and fuel reduction treatments on multiple, interacting forest stresses of fire, insects, wind, and climate change.     

Monitoring of heavy metals in protected forest catchments in Sweden

Long-term integrated catchment monitoring within the Swedish Environmental Monitoring Programme (PMK) aims primarely at the fate and effects of pollutants in mature ecosystems, mainly protected boreal forests. The measurements that were conducted since the early eighties, with some variation, included deposition, throughfall, litterfall and the chemistry of soil water, groundwater and runoff water.Together with, or perhaps partly due to acidification, accumulation and transport of heavy metals are a serious threat to the ecosystem and in the end to the health of human beings. This paper discuss the behaviour of Hg, Pb, Cd, Zn and Cu based on the experiece gained from the studies in the small catchment areas. The importance of present deposition, soil storage and biogeochemical condition for metal mobility to the transport of metals is discussed The storages of Hg and Pb have been built up during a long time span and are very large in comparison with the flux. The present atmospheric deposition have probably little impact on the flux, which rather is governed by the biogeochemical conditions. There is still a small accumulation of Cd, but its great mobility under acidic conditions makes input and output almost in balance. A reduction in deposition will probably have an immediate effect on flux, which also is the case for Zn. Cu generally accumulates in soil. The mobility and hence the flux are certainly regulated by the biogeochemical conditions, but more detailed studies are required to reveal the general mecanisms for Cu transport within the catchment.     

The tyranny of taste: the case of organic rice in Cambodia

Fair-trade and organic products are often sold at price premiums justified by smaller production volumes that are associated with greater social and environmental responsibility. The consumption of these products confers on the consumer a greater sense of morality – and usually a claim to better taste. This paper tells the story of attempts to promote organic/fair-trade rice production by de facto organic Cambodian farmers for export to North American and European markets in order to assist poor farmers to trade their way out of poverty. It demonstrates that instead of promoting sustainable agriculture and fair trade between developed and developing markets, organic/fair-trade projects may impose First World consumer ideals and tastes that are out of step with the larger realities of agrarian transition in Cambodia and the wider region of developing Southeast Asia.     

Heat Transfer Performance of Capillary Mats Using Wet Construction in Winter

For the capillary mats using wet construction, a winter experiment designed to investigate the heat transfer performance was described, and the heat transfer performance analysis which contains the comparisons between different pipe space and different thickness of cement mortar surface was carried out. As a result, compared with that of the traditional radiant heating pipes, the capillary mats of wet construction require supply water with lower temperature, and it shows good heat transfer performance and system energy efficiency as well. According to the demand of indoor heat dissipation and human comfort as well as the coupling relationship between the temperature of supply water and surface construction thickness, it is suggested that the proper construction method should be comprehensively considered in practical applications.