Effects of remifentanil on monophasic action potential and transmural dispersion of repolarization in rabbit myocardium

AIM: To study the effect of remifentanil on monophasic action potential and transmural dispersion of repolarization (TDR) in the 3-layer myocardium of isolated rabbit hearts. METHODS: Adult rabbits (n=18, 2.0 ~ 2.5 kg) were used to isolate the hearts for preparing Langendorff perfusion model. The hearts were randomly divided into 3 groups after perfusion with K-H solution for 15 min: the perfusion in control group (C group) continued for 60 min; the hearts in remifentanil group (R group) were perfused with 12 μg/L remifentanil K-H solution for 60 min; the hearts in remifentanil+aminophylline group (RA group) were given 60-min perfusion of 12 μg/L K-H remifentanil+30 mg/L aminophylline. The HR and 3 layers of myocardial monophasic action potential (MAP) in the left ventricular anterior wall were recorded at time points after balanced infusion for 15 min (T₀), and continued perfusion for 15 min (T₁), 30 min (T₂) and 60 min (T₃). The monophasic action potential duration of repolarization at 90% (MAPD₉₀) and the transmural dispersion of repolarization (TDR) were calculated. The early afterdepolarization, delay afterdepolarization and arrhythmia were also observed. RESULTS: In R group, slower HR and prolonger MAPD₉₀ and TDR at T₁~T₃ were observed as compared with those at T₀ (P<0.05). R group showed slower HR and longer MAPD₉₀ and TDR than C group and RA group (P<0.05). CONCLUSION: Remifentanil slows the HR, extends the MAPD₉₀ and increases the TDR, thus being prone to induce reentry. Aminophylline makes HR faster and MAPD₉₀ shorter, thereby reducing the TDR.     

不同种源砂生槐种子萌发特性的比较研究

以3个不同种源的砂生槐种子为研究对象,对种子外部形态特征、千粒重及不同处理条件下其发芽率和发芽势进行了比较分析.结果表明:各种源砂生槐种子的横纵径差异不明显,千粒重之间有差异,其中,日喀则种源千粒重最大.拉萨种源在各水温浸泡后其发芽率较其余2个高,达到42％,3个种源的砂生槐种子随着浸泡水温的升高,其发芽率也表现出逐渐升高的趋势,均表现为沸水浸泡后发芽率达到最大.3个种源的砂生槐种子在不同温度下发芽率不同.温度越高并不表现为发芽率较高,其中拉萨和林芝种源的砂生槐种子在15℃时发芽率最好,日喀则种源在20℃发芽率最好.GA3对砂生槐种子的萌发没有明显的促进作用.切破种皮和酸碱处理均有利于不同种源砂生槐种子的萌发,能在一定程度上提高砂生槐种子的发芽率.     

汞水平对苋菜种子萌发的影响

采用不同浓度的氯化汞溶液(0.5、1.0、2.5、5.0、10.0、25.0、50.0、100.0 mg/L)对不同品种苋菜种子进行浸种处理。结果表明,Hg+对苋菜种子萌发的影响在低浓度时有促进作用,随着Hg+浓度增加,苋菜种子的根长和芽长都明显减少,当Hg+浓度达到100 mg/L时,苋菜种子见萌芽,但都有红褐色痕迹,即汞污染现象非常明显;Hg+较高浓度时,各个不同品种苋菜种子导电率相对较高,种子活力相对较低。     

Geostatistical interpolation and aggregation of crop growth model outputs

Many crop growth models require daily meteorological data. Consequently, model simulations can be obtained only at a limited number of locations, i.e. at weather stations with long-term records of daily data. To estimate the potential crop production at country level, we present in this study a geostatistical approach for spatial interpolation and aggregation of crop growth model outputs. As case study, we interpolated, simulated and aggregated crop growth model outputs of sorghum and millet in West-Africa. We used crop growth model outputs to calibrate a linear regression model using environmental covariates as predictors. The spatial regression residuals were investigated for spatial correlation. The linear regression model and the spatial correlation of residuals together were used to predict theoretical crop yield at all locations using kriging with external drift. A spatial standard deviation comes along with this prediction, indicating the uncertainty of the prediction. In combination with land use data and country borders, we summed the crop yield predictions to determine an area total. With spatial stochastic simulation, we estimated the uncertainty of that total production potential as well as the spatial cumulative distribution function. We compared our results with the prevailing agro-ecological Climate Zones approach used for spatial aggregation. Linear regression could explain up to 70% of the spatial variation of the yield. In three out of four cases the regression residuals showed spatial correlation. The potential crop production per country according to the Climate Zones approach was in all countries and cases except one within the 95% prediction interval as obtained after yield aggregation. We concluded that the geostatistical approach can estimate a country’s crop production, including a quantification of uncertainty. In addition, we stress the importance of the use of geostatistics to create tools for crop modelling scientists to explore relationships between yields and spatial environmental variables and to assist policy makers with tangible results on yield gaps at multiple levels of spatial aggregation.     

Glycine max and Glycine soja are capable of cold acclimation

Soybean has been considered a cold intolerant species; based largely upon seed germination and soil emergent evaluations. This study reports a distinct acquisition of cold tolerance, in seedlings, following short acclimation periods. Diversity in cold responses was assessed in eight cultivars of Glycine max and six accessions of G. soja. All varieties of soybean significantly increased in freezing tolerance following acclimation. This study indicates soybean seedlings are indeed capable of sensing cold and acquiring cold tolerance. Germination rates after cold imbibition were negatively correlated with maturity group, but positively correlated with cold acclimation potential in G. soja. Seed fatty acid composition was varied between the species, with Glycine soja accessions containing about 2‐times more linolenic acid (18:3) than G. max. Furthermore, high levels of linoleic acid (18:2) in seeds were positively correlated with germination rates following cold imbibition in G. soja only. We suggest that domestication has not impacted the overall ability of soybean to cold acclimate at the seedling stage and that there is little variation within the domesticated species for ability to cold acclimate. Thus, this brief comparative study reduces the enthusiasm for the “wild” species as an additional source of genetic diversity for cold tolerance.     

Effect of Psammaplin A on in vitro Development of Bovine Aging Oocytes

To investigate the effect of histone deacetylation inhibitor Psammaplin A (PsA) on the development of bovine aging oocytes in vitro,oocytes were randomly divided into control group,aging group and 50 mmol/L PsA treated aging group (PsA group).Immunofluorescence staining and JC-1 were used to detect the blastocyst rate of bovine oocytes after parthenogenetic activation,the number of cells in blastocysts,apoptosis,reactive oxygen species (ROS),glutathione (GSH) and mitochondrial membrane potential intensity of embryos.The results showed that the blastocyst rate of the aging group was significantly lower than that of PsA and control groups (P<0.05).The blastocyst rate of PsA group was not significantly different from that of control group (P>0.05).The number of cells in the blastocysts of control group and PsA group were significantly higher than that of aging group (P<0.05).The number of cells in the blastocysts of PsA group was not significantly different from that of control group (P>0.05).The apoptosis rate in aging group was significantly higher than that of control and PsA groups (P<0.05),the apoptosis rate of PsA group was significantly higher than that of control group (P<0.05).The GSH level of MⅡ oocytes in aging group was significantly lower than that of control and PsA groups (P<0.05).There was no significant difference in GSH level between control and PsA groups (P>0.05).The ROS level of the embryos in aging group was significantly higher than that of control and PsA groups (P<0.05).The ROS level in PsA group was significantly higher than that of control group (P<0.05).The mitochondrial membrane potential of early embryos of aging group 4-8 cells was significantly lower than that of control and PsA groups (P<0.05).The mitochondrial membrane potential intensity of control group was significantly higher than that of PsA group (P<0.05).In summary,PsA could effectively delay the aging of bovine oocytes and improve the quality of oocytes.     

无瓣海桑种子特征及不同贮藏环境下萌发特性的研究

种子的萌发除了与自身的生理结构、萌发时外界条件有关外,不同的贮藏条件和时间处理对种子萌发也有影响.本实验以无瓣海桑种子为研究对象,通过无瓣海桑种子的基本特征及不同贮藏环境(常温、常温干燥、-4℃、-70℃分别对应Ⅰ、Ⅱ、Ⅲ、Ⅳ)和时间(15,30,45,60,90 d)下其种子萌发特性的分析发现:径级对无瓣海桑的果重和种子的粒数均有显著影响;低温处理可显著缩短种子的萌发时间,同时有助于保持无瓣海桑的种子活性;无瓣海桑种子的活力会随着贮藏时间的增加而下降,因此不适宜长期贮藏,但短期贮藏时低温干燥贮藏可较好地保持其种子活力.     

Optimum Soil Water Content for Chickpea Emergence in Heavy‐Textured Soils of North‐West Bangladesh

Sowing of chickpea in the heavy‐textured soils of north‐west Bangladesh with minimum tillage technology aims to increase the timely planting of large areas during a relatively short sowing window before soil water deficit limits germination and emergence. However, the seedbed conditions into which chickpea is sown need to be better quantified, so that limiting factors which affect germination and emergence can be identified. Two of the soil physical characteristics of importance are soil water and aeration. Growth cabinet studies have identified the fastest germination and emergence of chickpea on representative soils for this area at gravimetric water contents of 17–18 %, whilst soil water contents above and below this delayed germination and emergence. Emergence was recorded at soil water potentials between field capacity (?10 kPa) and wilting point (?1500 kPa). Emergence was possible at lower soil water potentials in the finer textured soil, whilst in coarser textured soil, emergence was still possible at higher soil water potentials.     

NaCl胁迫对白蜡种子生理特性的影响

为了给盐碱地造林树种选择提供依据,以采自秦皇岛和保定的白蜡种子为试材,采用不同浓度的NaCl胁迫处理,研究了其萌发过程中发芽率、细胞膜透性、保护酶活性、丙二醛MDA的变化,结果表明:(1)随着NaCl胁迫浓度的增加,两地白蜡种子发芽率均呈现逐渐降低的变化趋势,秦皇岛各处理的白蜡种子发芽率分别比对照降低了8.9%、52.6%、56.3%、61.6%、87.9%、91.1%,而保定的白蜡种子分别比对照降低20.2%、56.2%、68.7%、86.5%、89.9%、100.0%;(2)随着浓度的升高,种子的相对电导值、K+、Na+渗透率升高,种子的SOD酶活性降低,MDA含量增加,NaCl胁迫浓度越高,种子所受到的伤害越深;(3)0.20%浓度的NaCl胁迫对白蜡种子萌发影响不大。对于不同种源的白蜡种子,秦皇岛的白蜡种子抗盐性要优于保定的白蜡种子。     

四种柱花草种子萌发中参与氮代谢的酶活性变化

研究了4种柱花草种子萌发过程中参与氮代谢的蛋白酶、肽酶和硝酸还原酶活性的动态变化。结果表明,蛋白酶的活性随着柱花草种子萌发的深入而不断上升,在种子即将露白胚根突破种皮的时候蛋白酶活性达到最大值。此后,随着胚芽的生长蛋白酶活性逐渐降低,其中热研2号柱花草的蛋白酶活性高于其他3种;肽酶活性的变化总体呈上升趋势;硝酸还原酶呈S型变化。了解柱花草种子萌发期各种酶活性的变化规律,为深入研究柱花草种子萌发时贮藏物质的动员和代谢机理提供数据。