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51.
肾上腺皮质肿瘤(Adrenocortical Tumor,ACT)是一种常见的肿瘤,存在于人体的内分泌系统中,并且是引发肾上腺皮质癌(Adrcnocortical Carcinoma,ACC)进而导致死亡的主要原因.经典的"Weiss系统"在诊断明确性上存在种种无法解决的问题,但随着科技水平的发展,人们开始探索新的途径来鉴别ACT的良恶性,于是开始有了影像学和分子生物学技术等特异性的检查方法,从而进一步增强了诊断的明确性.现通过对比的方式,探究肾上腺皮质肿瘤(Adrenocortical Tumor,ACT)影像学检查和分子生物学技术检查的结果,从而能更准确地鉴别其良恶性. 相似文献
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为了解贵州省三穗县鸭疫里默氏杆菌(Riemerella anatipestifer,RA)的流行血清型、毒力和耐药情况,本试验从贵州省三穗县6个规模养鸭场临床疑似RA感染的病鸭体内分离出6株分离菌,对病原菌进行分离鉴定、毒力基因检测和耐药性分析。细菌分离鉴定结果显示,分离菌在巧克力琼脂培养基上长出表面光滑、圆形半透明的滴状菌落;革兰氏染色呈阴性短小杆菌,瑞氏染色呈两极浓染;分离菌均不具运动性,尿素、触酶和氧化酶试验均为阳性,符合RA生化特性,将6株分离菌分别命名为SS-RA1~SS-RA6;6株分离菌的16S rRNA基因序列与NCBI上RA参考菌株的基因序列相似性≥98%,说明6株分离菌均是RA;SS-RA1~SS-RA4为血清2型且含有8种毒力基因(OmpA、CAMP、wza、AS87_04050、Fur、SIP、TbdR1和luxE基因),SS-RA5和SS-RA6为血清11型,缺失AS87_04050基因,仅含有上述其余7种毒力基因;动物回归试验结果显示,攻毒组雏鸭均全部死亡,对照组雏鸭未表现明显临床症状,表明6株分离菌对雏鸭均有致病力;药敏试验结果显示,6株分离菌仅对羧苄西林、哌拉西林、头孢他啶、头孢氨苄、头孢曲松、头孢拉定和头孢哌酮7种抗菌药物敏感,对其他13种抗菌药物均表现不同程度的耐药,对氨基糖苷类和大环内酯类抗菌药物的耐药率为100%。本试验成功分离得到6株RA,为贵州省三穗县鸭疫里默氏杆菌病的疫苗选择和药物防治提供理论依据。 相似文献
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近年来甘肃陇南地区华山松溃疡病发生严重,导致华山松大面积死亡,但有关病害发生的原因等尚不清楚。采用组织分离法从感病华山松的健康部位、病健交界处、子实体及病组织处分离获得97个真菌菌株,结合形态学及系统学对分离到的菌株进行初步分析。结果显示,所得菌株分为9个种,其中Neocosmospora silvicola占14.17%,Fusicolla violacea占1.67%,Microcera sp.占8.33%,Neonectria neomacrospora占11.67%,Dermea pinicola占8.33%,Nematogonum ferrugineum占9.17%,Pezicula sporulosa占4.17%,Phaeoacremonium fraxinopennsylvanicum占14.17%,Sarea resinae占9.17%。以丛赤壳科为优势菌群,占总菌株数量的44.33%;初步推断Neocosmospora silvicola为疑似病原菌。 相似文献
56.
ZHANG Zhi CHENG Xiao-na FAN Ya-ting DONG Ya-qin LIU Shuang WU Fa-xing SHAO Wei-xing LI Xiao-cheng 《中国畜牧兽医》2015,42(7):1654-1660
To ascertain a diarrhea case in a pig farm in Shandong province,the pathological changes of dead piglets were observed and nested RT-PCR test was carried out on 7 diarrhea samples for porcine epidemic diarrhea virus (PEDV).Pathological examination revealed that intestine was detected as enlargement,hyperemia and edema.After histological examination,the typical microscopic lesions of intestine were disappearance of epithelial cells,villus shrinkage and shortening.The result of nested RT-PCR showed that all of the 7 samples could amplify a specific target band of PEDV.Molecular characteristics of two field strains showed that they had an amino acid homology of 92.3% to 92.4% with vaccine CV777 and 96.6% to 98.6% with other previous field strains which sequences were downloaded from GenBank.Phylogenetic tree analysis further revealed that all of PEDV strains could be mainly divided into two clusters of G1 and G2. G2 consisted of the field strains of our study and other field strains from USA,China and so on,which had the same sequence characteristics of two insertions and one deletion,while G1 consisted of all vaccines of CV777 and several older field strains from China and Korea.These results indicated that our field strains were the dominant strains in recent epidemic diarrhea occurrence.Moreover,its molecular characteristics might be a characterization of differentiating the field and vaccine strains. 相似文献
57.
58.
Pheromones are communication chemicals and regulatory signals used by animals and represent unique tools for organisms to mediate behaviors and make “decisions” to maximize their fitness. Phenotypic plasticity refers to the innate capacity of a species to tolerate a greater breadth of environmental conditions across which it adapts to improve its survival, reproduction, and fitness. The pinewood nematode, Bursaphelenchus xylophilus, an invasive nematode species, was accidentally introduced from North America into Japan, China, and Europe; however, few studies have investigated its pheromones and phenotypic plasticity as a natural model. Here, we demonstrated a novel phenomenon, in which nematodes under the condition of pheromone presence triggered increased reproduction in invasive strains (JP1, JP2, CN1, CN2, EU1, and EU2), while it simultaneously decreased reproduction in native strains (US1 and US2). The bidirectional effect on fecundity, mediated by presence/absence of pheromones, is henceforth termed pheromone-regulative reproductive plasticity (PRRP). We further found that synthetic ascaroside asc-C5 (ascr#9), the major pheromone component, plays a leading role in PRRP and identified 2 candidate receptor genes, Bxydaf-38 and Bxysrd-10, involved in perceiving asc-C5. These results suggest that plasticity of reproductive responses to pheromones in pinewood nematode may increase its fitness in novel environments following introduction. This opens up a new perspective for invasion biology and presents a novel strategy of invasion, suggesting that pheromones, in addition to their traditional roles in chemical signaling, can influence the reproductive phenotype among native and invasive isolates. In addition, this novel mechanism could broadly explain, through comparative studies of native and invasive populations of animals, a potential underlying factor behind of the success of other biological invasions. 相似文献
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60.
为研究芥菜型油菜的耐旱性,对150份云南芥菜型油菜地方品种的品质及苗期不同干旱程度下植株地上
及地下部进行研究。结果表明,参试芥菜型油菜耐旱性总体表现较好,品质变异丰富,其中油酸、芥酸变异系数大
于1,依据品质性状参试品种大致聚为三类;中度及重度干旱均能显著影响油菜地上部及根系的生长,其中对根系
影响更大;各品质性状与耐旱性相关分析表明,中度干旱条件下,耐旱性与含油量及芥酸、花生烯酸2种极长链脂肪
酸呈显著正相关,与5种长链脂肪酸硬脂酸、棕榈酸、油酸、亚油酸、亚麻酸为显著负相关,而重度干旱条件下均不相
关,同一品种在不同程度干旱下其耐旱性有差别;含油量与极长链脂肪酸呈显著正相关,与长链脂肪酸及蛋白含量
呈显著负相关,极长链脂肪酸与长链脂肪酸间呈极显著负相关。共筛出8个综合耐旱性强的材料。 相似文献